Marine Yeast Pigmentation Essay
RESULT AND DISCUSSION
In this study the marine yeasts were isolated and screened for its pigment production. A total of 30 Marine yeast strains were isolated from Kelambakkam saltpans, Mangrove sediments, Mangrove water sample, sea water and mangrove leaf sediment sample, nearby East Coast of Tamil Nadu, India and they were named as AMBY101 to AMBY130. Among the 30 of the isolated colonies 3 were capable of producing pigments they are AMBY101, AMBY109 and AMBY130.The morphological characteristics of these pigment producing strains was characterized by the morphological appearance and the standard procedures (Table 1).
After the morphological and biochemical characteristics the pigment producing strains were inoculated in YM media and extracted using methanol. The coloured pigment extracts was collected and the OD value was taken at 490nm and 660nm. The degree of pigmentation was calculated and found the highest pigment producing isolate was AMBY109 (Table 2).
The optimization of the potential pigment producing strain was done.The
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Natural colours are abundant in microorganisms such as bacteria, fungi , yeast ect., among which Yeast are readily grown and are easy to cultivate in large scale level. Thus the current study was focused on isolation of pigment producing yeast and found that 3 strains are capable of producing pigments. The potential strain was optimized under several conditions by response surface methodology and extracted using methanol. Antibacterial activity was done against different human pathogens and finally it was checked in test tubes and plates to verify its absorption level and found that the pigment produced from the Rhodotorulaspis readily absorbable in agar and further study of this pigment canlead to an effective natural food colourant in food
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