Marine Shrimp Case Study

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DNA Barcode sequence of marine ornamental Squat shrimp Thor amboinensis using mtCO1 from Gulf of Mannar coastal waters

V.Priyalakshmi1, S.Dhanasekaran1, M.A.Badhul Haq2*, M.Nirosh Banu2, S.Vaitheeswari2 and P.Vengadesan2
1P.G and Research Department of Zoology, Yadava College, Madurai Kamaraj University,
2Marine Virology Laboratory, Faculty of Marine Sciences,
CAS in Marine Biology, Annamalai University, Parangipettai - 608 502, Email*:drhaqmarinevirology@gmail.com

Abstract

Marine shrimp are among the most popular invertebrates in the marine ornamental aquarium trade. In this study exclusively focused in addressing the phylogenetic location of genus Thor amboinensis. A molecular phylogeny of the marine ornamental shrimp species based on
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The phylogeneic in sequence and evolutionary relationship to species of cleaner shrimp Thor amboinensis to evaluating the promise and molecular taxonomy of individuals comparative to described taxa and DNA led discovery of novel species. Those aspects are rely on a solid taxonomic base, including sufficient sampling of disparity within species and enclosure proves the efficacy of mtDNA CO1 gene sequence based approach in recognizing fish species at a earlier pace.

Acknowledgements
The authors are thankful to the supports form SERB Sanction order No. SR /FT / LS-125/2011 and University Grants Commission, Govt of India research project No: F. No: 41- 4/2012 (SR) for providing funds and authority of Annamalai Unveristy for providing facility for this
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S.No. Accession No. Country
1 FN392162 Guam:Mariana Island
2 GQ260961 Palmyra Atoll: Line Islands
3 HM464734 Australia: Heron Island, QLD

4 HM465917 Australia: Ningaloo Reef, WA

5 JQ180245 French Polynesia

Table 5. Accession numbers of sequences closely related to the Test organism used in the analysis & their locations.

S.No. Accession No. Country
1 AM083327 French Polynesia
2 AY135199 Brasil
3 AY344204 Brasil
4 AY344207 Brasil
5 AY344219 Brasil

Table .6 Nucleotide composition of Thor amboinensis & closely related sequences

Name of species Accession ID Base pair length G+C content
(%) A+T content
(%) Nucleotide Number and
Mol%
A T G C
MAB10 Test Organism 654 34.1% 65.9% 191
29.2% 240
36.7% 114
17.4% 109
16.7%
FN392162 657 31.7% 68.2% 196
29.8% 252
38.4% 108
16.4% 100
15.2%
GQ260961 658 33.9% 66.1% 191
29.0% 244
37.1% 114
17.3% 109
16.6%
HM464734 658 30.7% 69.3% 191
29.0% 265
40.3% 113
17.2% 89
13.5%
HM465917 658 30.7% 69.3% 191
29.0% 265
40.3% 113
17.2% 89
13.5%
JQ180245 614 34.2% 65.8% 171
27.9% 233
37.9% 121
19.7% 89

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