DNA Barcode sequence of marine ornamental Squat shrimp Thor amboinensis using mtCO1 from Gulf of Mannar coastal waters
V.Priyalakshmi1, S.Dhanasekaran1, M.A.Badhul Haq2*, M.Nirosh Banu2, S.Vaitheeswari2 and P.Vengadesan2
1P.G and Research Department of Zoology, Yadava College, Madurai Kamaraj University,
2Marine Virology Laboratory, Faculty of Marine Sciences,
CAS in Marine Biology, Annamalai University, Parangipettai - 608 502, Email*:drhaqmarinevirology@gmail.com
Abstract
Marine shrimp are among the most popular invertebrates in the marine ornamental aquarium trade. In this study exclusively focused in addressing the phylogenetic location of genus Thor amboinensis. A molecular phylogeny of the marine ornamental shrimp species based on
…show more content…
The phylogeneic in sequence and evolutionary relationship to species of cleaner shrimp Thor amboinensis to evaluating the promise and molecular taxonomy of individuals comparative to described taxa and DNA led discovery of novel species. Those aspects are rely on a solid taxonomic base, including sufficient sampling of disparity within species and enclosure proves the efficacy of mtDNA CO1 gene sequence based approach in recognizing fish species at a earlier pace.
Acknowledgements
The authors are thankful to the supports form SERB Sanction order No. SR /FT / LS-125/2011 and University Grants Commission, Govt of India research project No: F. No: 41- 4/2012 (SR) for providing funds and authority of Annamalai Unveristy for providing facility for this
…show more content…
S.No. Accession No. Country
1 FN392162 Guam:Mariana Island
2 GQ260961 Palmyra Atoll: Line Islands
3 HM464734 Australia: Heron Island, QLD
4 HM465917 Australia: Ningaloo Reef, WA
5 JQ180245 French Polynesia
Table 5. Accession numbers of sequences closely related to the Test organism used in the analysis & their locations.
S.No. Accession No. Country
1 AM083327 French Polynesia
2 AY135199 Brasil
3 AY344204 Brasil
4 AY344207 Brasil
5 AY344219 Brasil
Table .6 Nucleotide composition of Thor amboinensis & closely related sequences
Name of species Accession ID Base pair length G+C content
(%) A+T content
(%) Nucleotide Number and
Mol%
A T G C
MAB10 Test Organism 654 34.1% 65.9% 191
29.2% 240
36.7% 114
17.4% 109
16.7%
FN392162 657 31.7% 68.2% 196
29.8% 252
38.4% 108
16.4% 100
15.2%
GQ260961 658 33.9% 66.1% 191
29.0% 244
37.1% 114
17.3% 109
16.6%
HM464734 658 30.7% 69.3% 191
29.0% 265
40.3% 113
17.2% 89
13.5%
HM465917 658 30.7% 69.3% 191
29.0% 265
40.3% 113
17.2% 89
13.5%
JQ180245 614 34.2% 65.8% 171
27.9% 233
37.9% 121
19.7% 89
Nucleotide 1 2 3 4 5 6 7 8 9 A 0.286 0.357 0.143 0.071 0.071 0.500 0.643 0.071 0.143 C 0.357 0.143 0.214 0.071 0.071 0.071 0.071 0.143 0.143 G 0.143 0.214 0.500 0.786 0.071 0.143 0.214 0.714 0.214 T 0.214 0.286 0.143 0.071 0.786 0.071 0.071 0.071 0.500 3.3 Log-odds
Crayfish are decapods pertaining to the phylum arthropoda, which are invertebrates and contain an open-circulatory system.1 The system works by the hemolymph getting re-oxygenated in the gills before being transporting to the heart by brachio-cardiac veins and then pumped to sinuses that bathe tissues with oxygenated hemolymph.2 The crayfish contain a neurogenic heart that depends on neuronal input from cardiac ganglion.3 The neurogenic heart requires nerve impulses to produce contractions in contrast to a myogenic heart that can contract independently from the nervous system.3 The crayfish heart will beat due to the reaction of the ganglion to stimuli in the environment.4 Stimuli will affect the autonomic nervous system in the crayfish that control involuntary actions such as the heart rhythm.4 Neurotransmitters are the chemical signals in which the nervous system regulates both heart rate and contraction.3The autonomic nervous system breaks into two categories as parasympathetic system that is involved in relaxation of organs and the sympathetic system that will stimulate increased activity. Neurotransmitters can either increase or decrease heart rate by altering the patterns in neural activity of the heart.4 Crayfish are poikilotherms, which means they cannot metabolically thermoregulate thus conform to the ambient temperature in the water.
Genomic Recombination and Deletions in Acinetobactor baylyi ADP1 Shivani Patel Fall 2015 BIO 493 Introduction: Gene duplication and amplification is a process by which genetic diversity can be created and selected for. Through the understanding of gene duplication and amplification, scientists can garner insight on medical conditions associated with this phenomenon (Seaton et al. 2012). Not only can gene duplication and amplification increase genetic diversity, it can also increase the fitness of bacteria by allowing an increased production of essential nutrients or a gene to gain a new function (Dhar et al. 2014). However, gene amplification is not the only large genome change that can occur in organisms.
This could be due to enviornmental reasons. Due to the enviornment brine shrimp usually have to grow is due to natural selection. Through natural selection they are able to pass down genes and carry ablitly to reproduce. Unlike in our experiment where we just had a solution and them in a petry dish. Brine shrimp can live in an enviornment
“A righteous man regardeth the life of his beast: but the tender mercies of the wicked are cruel” (King James Version, Prov. 12.10). In order to fully regard the life of God’s beasts, we must preserve/facilitate their population so others can look and marvel at God’s creation. Information shows that seahorse populations are commonly vulnerable to heavy exploitation due to traditional Chinese medicine (TCM), aquarium trade, and destruction of natural habitats (Allee). The IUCN red list of threatened species tracks animal populations and classifies them based on conservation status. Many species of seahorse such as the Hippocampus Reidi are classified as data deficient; showing there is a lack of research and concern over these disappearing
Do you know how you got here? I’m not talking about how your parents met. I’m not talking about your past. I’m talking about evolution and how we all got here. In the passages I have just viewed “A Fish Out of Water” by Greg Pardo & “Dinomummy” by Ryan Overbee, I have learned that some information that we thought was true was been proven wrong.
Myrtle rust is an exotic rust fungus originating from South American region, detected for the first time in Australia (New south wales) on 22nd April 2010, the fungus was found growing on syncarpia glomulifera, callistemon viminalis and agonis fluxuosa plants. The infected plants can be easily identified from powdery bright orange-yellow or yellow spores on fruits, buds, leaves and shoots. As the rust fungus is considered to be a biosecurity threat, a state emergency response program was initiated by the Australian government, which conducted surveys in NSW in 2010, the data collected from the surveys shows that the fungus has already been recorded on 107 host species in 30 genera. Understanding the genome: Advanced molecular biology techniques
The results of the gel electrophoresis are summarized in Figure 2. On the gel, both populations of fish were run and analyzed for their heterozygosity. Following the conclusion of the electrophoresis, the gel was analyzed to determine how many different alleles were present at the SFMSTR5 loci. The results of the analysis are shown in Figure 2. The gel showed that in population 1, there are three different alleles at the SFMSTR5 loci and that a majority of the fish in this population are heterozygous at this locus.
Even though mantis shrimp are colorful and interesting to look at, they are very, very dangerous to handle and should be avoided without proper handling equipment. Some may call them a “living knife”. Fishermen see the shrimp a lot when getting their nets out of the water because they like living at the bottom of the ocean and the nets ruff the bottom
Cobia, Rachycentron canadum Introduction: Cobia, Rachycentron canadum, is the only species of the family Rachycentridae. Other common names for cobia include black kingfish, black salmon, lemonfish, and sergeant fish as well as cobie and bonito (in Spanish) and mafou (in French). Cobias are a popular recreational and commercial species. They reach lengths of 50-120 cm, with a maximum of 200 cm.
The Keepers from the Plankton Factory are being charged with child abuse. Esperanza Mendoza the leader of the Convent in San Lois caught the Keepers beating up children and duct taped one other children. Some kids testified against the Keepers. Most of the kids say that Jorge Martinez had beaten some of the kids that work there. Fidelito Lopez states, “I was there, Jorge had grab a cane out of a closet and he was going to beat me with it, but Matt had came out and took the cane from Jorge.
By Gram staining alone, it was safe to eliminate the three Gram positive bacteria that could have been assigned: S. epidermidis, M. luteus, and B. megaterium. The second step was to streak plate Unknown #10 to observe its macroscopic
My favorite foods are shrimp and peanuts. These two foods are my favorite because they are both healthy and they're both really delicious. Shrimp also belongs to my favorite food group, seafood. Peanuts are also full of salt, which is the part of the peanuts I like the best. Shrimp and peanuts are both good for you and they are both very filling.
Marine organisms are animals, plants, and other living things that live in the ocean. A Marine biologist is a scientist who studies marine organisms and studies the bodies, behavior, and the history of marine organisms. They also study how marine organisms interact with each other and their environment. I have chosen to research about Marine biology because I would like to learn about sea life, the ocean, and its surrounding environment. To start off, a Marine biologist might study coral, crabs, fish, microscopic marine organisms, sea stars, seaweed, squid, or whales.
The second group, the tilapiines, consists of the only substrate-spawning species in the lake (Tilapia rendalli), and four species of chambo