Practical Investigation:
Marula Wine Experiment
Angus McQueen
Introduction:
Chemical Process of Fermentation of Marula Fruit:
The fermentation of alcohol involves converting large organic molecules (such as carbohydrates and glucose) into more straightforward molecules, like alcohols and carbon dioxide. For fermentation to occur bacteria and yeast are needed. There should be anaerobic conditions for the process to occur. This means that the process occurs without air. Therefore oxygen may not be used in this process. Fermentation is an exothermic reaction where heat is given off as a result of the reaction that occurs.
During the alcoholic fermentation a basic reaction occurs:
Sugar + Yeast Enzymes = CO2 + Alcohol
The
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The predominant factor that should be controlled is the temperature. If the temperature readings are too low or too high the yeast will be break down thus preventing fermentation from occurring.
During the production of alcohols, sugar is converted into carbon dioxide. This can be seen as a foam layer that resides on the top. As soon as the bonds in the sugar break down, carbon dioxide forms and bubbles through the solution. It is here where it is lost in the atmosphere. This then allows the alcohol to remain in the solution. An excess of alcohol in the solution causes the yeast to die.
Hypothesis:
The addition of artificial ingredients will degrade the quality of a marula wine.
Method:
Ingredients for Wine A: (Use of artificial ingredients)
• 2.5 Kg ripe marulas
• 1.5 Kg white sugar
• ½ a teaspoon of sodium metabisulphite
• 2.5 Litres of water
• 20 Grams of Brewer’s yeast
• 1 tablet vitamin B Ingredients for Wine B: (Use of natural ingredients)
• 2.5 Kg ripe marulas
• 250 ml of water Method for wine A: (artificial
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There was not enough time for the process to ferment fully. Lack of research prior to the experiment is the result of a few failed attempts in the process of making the wine. Other than that there was little to no experimental error.
Recommendations for improvement:
• Score the two different wines separately in order to compare them. This is to decipher which method is proven more successful.
• Time is a very important aspect, more time was needed for the fermentation to take place. With a limited time frame our wine could not ferment fully therefore resulting in the below average scores for colour and clarity. Therefore a longer fermentation period is recommended.
Conclusion:
The addition of artificial ingredients did degrade the quality of the wine. This is because the marula fruit is sweet as it is and the addition of artificial ingredients just made it too sweet. The wine with the natural ingredients tasted better because of this.
Front and back view of the
A lot of lesson in this experiment, one is that people like different flavor of gum for different reasons, and two not all Gums are the same. Our possible source of error is that we could
Another critical problem with sample processing and analysis is cold settling the wild yeast. After collecting berries from the valley floor, interns cold settle berries at 8 degrees Celsius for 48 hours. They also cold settle berries harvested from the hillside for 20 hours. However if the temperature is too low, the yeast would be "cold shocked"; although low temperatures may help to preserve the aroma but it will slow the activity rate of yeasts, thus resulting in the lag phase and fermentation activity will be stuck. When fermentation is stuck, microorganism (yeast) growth rate will be reduced and they will minimally consume sugar to convert them to ethanol.
A Demonstration of Chemotaxis Between Flies and Various Substances (Sugar vs Bacteria) Abstract: The purpose of this lab based on the Drosophila melanogaster (the common fruit fly) reactions. Since the fly has been studied and observed for many years, and known for its unique chemotactic attractions to different stimuli, it was an ideal organism for the study being conducted. In the experiment the purpose was to be able to figure out whether the flies would be more attracted to sugar or bacteria.
The third problem is the difference between the tastes
Through Eavan Boland 's poetry, she looks back on things that have happened in her life, pondering their meaning and how they have impacted her for good or bad. She does this through her use of language. There are recurring themes in her poetry. She refers to mythology and folklore and compares them to life experiences. Boland uses simple and accessible language even when dealing with complex emotions.
There are several reactions occur when there is plenty of oxygen present. Then the energy released is used by the yeast for growth and activity. However, when the oxygen supply is limited, the yeast can only partially breakdown the sugar. Alcohol and carbon dioxide are produced in this process known as alcoholic fermentation. The fermentation occur when the carbon dioxide produced in these reactions.
1% glucose, 1% maltose and 1% lactose all progressively get positive results by changing colours to reddish brown at the end of this experiment. In this case the aldehyde functional group that is present in the products (monosaccharides and some disaccharides) in this reaction is able to reduce copper in the presence of alkali and this produces colour changes while converting to an aldose sugar. Honey is made of fructose and glucose which instantly turned brown after the test-tube was placed in the boiling water because of its active aldehyde and carbonyl group. The copper (II) sulphate present in the Benedict’s solution reacts with electrons from the aldehyde group which results in a redox reaction to from cuprous oxide, a red brown precipitate that seen in all of the above mentioned solutions (Hill, 1982). Beer also gave positive results because it contains aldehydes and ketones (i.e. acetone, trans-2-butenal, furfual) during its beer production process where the sugars are converted through fermentation (Hill, 1982).
3. In this experiment, the percent yield was 90%. This number implies that there was little error in this experiment. However, this result could have been caused by certain external factors.
Title: How Ph Levels Affected the Fermentation of Beer Hypothesis: The beer will be left with more sugar deposit as the Ph levels increase because alpha/beta -amylase will no longer function. Predictions: Alcohol Percentage Analysis for the Control and the Experimental During this experiment, the pH level was increased, therefore Alpha-Amylase was favored. Due to the nature of Alpha-Amylase cutting randomly through a large carbohydrate molecule, it leaves bigger sugars in the flask, which cannot be digested by yeast. Due to this, less reactions should occur in the experimental, therefore leading to a lower percentage of alcohol production, compared to the control.
For example, fermentation occurs in yeast in order to gain energy by transforming sugar into alcohol. Fermentation is also used by bacteria, they convert carbohydrates into lactic acid. Ethanol fermentation is done by yeast and certain bacteria, when pyruvate is separated into ethanol and carbon dioxide. Ethanol fermentation has a net chemical equation: C6H12O6 (glucose) > 2C2H5OH (ethanol) + 2CO2 (carbon dioxide). This process of ethanol fermentation is used in the making of wine, bread, and beer.
Background Information: Yeast fermentation is directly affected by the change in temperature, because the rate of chemical reactions is affected by temperature. If the yeast has been exposed to its optimum temperature (66.667 degrees Celsius) then it will give off the highest carbon dioxide production. As the temperature gets higher, the yeast will produce more carbon dioxide, until at some point carbon dioxide production will decrease, that is when the yeast cells have become denatured due to the increase in temperature. Chemical reactions
The effects of alcohol on Biological Membranes. Introduction In this experiment it will be analysed the damage alcohols can have on biological membranes. Membranes are made up of lipids and proteins. Membranes usually help maintain the balance in a cell as it holds all the cellular materials.
Sugar/ glucose is an important carbohydrate that can be made during photosynthesis from water and carbon dioxide, using energy from sunlight. Carbon dioxide is given off as a waste product when energy is released by the breaking down of glucose. This can be used by plant cells in the process of photosynthesis to form new carbohydrates. Yeast is a single-celled fungus that can break down sugars (glucose) to help produce carbon dioxide. Research Question
For example, in the response experiment, a yeast solution was prepared without sugar mistakenly and thus had to be prepared again. This suggests that other errors in preparation and measurement could have been encountered. For the future, careful measurements using clean uncontaminated flasks would eliminate possibilities of such error. A source of error for the metabolism experiment involves the yeast’s yellow hue. It is possible that the color of the yeast caused the solution to look more
This method which uses an internal standard and flame ionisation detector, is exact and more specific than methods usually used. The gas-liquid chromatography method determines ethanol clearly and separately from the other beverage components that would have interfered in other methods, without any distillation or need for a chemical reaction. Determination of ethanol is one of the most vital routine analysis in a current winery. This method provides frequent, rapid and accurate results are needed to regulate the quality of the wine from grape to bottle, as well as for state and federal government