Melatonin Experiment

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Agami R. A (2014) studied the role of melatonin in barley plants under saline condition. An experiment was performed with 1 mM melatonin solution to study its effect on growth and yields of barley plants under salt stress (100 or 200 mM NaCl). The barley plants exposed in salt stress reduced all the growth parameters but the treatment through melatonin in salt stress enhanced all the growth and yield parameters.
An experiment was performed by sterilizing the Brassica juncea seed with 3 % hypochloros acid for thirty minutes. Then seedlings were placed into petri plates on filter paper with different melatonin concentrations of 100, 10, 1 and 0.1 mM in 0.02% dimethylsulfoxide. Control plants contained only distilled water. The results indicated …show more content…

(2016) determined the effect of melatonin in Zea mays seedlings in drought stress. The maize seedlings were foliar-sprayed with 100 μmol/L melatonin. It was noted that melatonin had no effect in normal situation. Under drought condition the growth of mays seedlings significantly reduced nut this reduction controlled by foliar expression of melatonin. Melatonin treated seedlings improved their earlier reduction quickly than non treated seedlings. Melatonin enhanced the rate of photosynthesis and transpiration under drought stress. It was also determined that drought stress increased the H2O2 and MDA contents but melatonin expression decreased this …show more content…

The seeds of two wheat genotypes (Sarsabz and S-24) will be collected from Ayub Agricultural Research Institute Faisalabad. The experimental design will be completely randomized with factorial arrangement. The seeds of wheat genotypes will be sown in soil with salt stress (control and 10 dS/m) and different melatonin treatments (0, 50, 100, 300 and 500 µM) will be foliarly applied.

Morphological Parameters
Shoot and Root length of the plant will be calculated by meter rod in centimeters. The fresh and dry weight of shoot and root will be determined in kilogram by using the electrical balance.
Chlorophyll contents
Chlorophyll contents (chlorophyll a, chlorophyll b, total chlorophyll and carotenoid contents) will be determined by the method of Arnon 1949.
Biochemical

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