Sample injection system This allows introduction of the analyte mixture into the stream of the mobile phase before it enters the column. Sample injector is made by means of a syringe and self-sealing septum of silicone, neoprene, or Teflon. Modern injectors are auto samplers, which allow programmed injections of different volumes of samples that are withdrawn from the vials in the auto sampler tray. 5. Column It is heart of HPLC system made up of heavy-walled glass tubing or precision-bore stainless steel.
Preservative free dexmedetomidine is available in 1ml and 2 ml ampoule as Dexmedetomidine Hydrochloride for intravenous use (Alphadex, Fusion health care 100 μg/ml). It can also be used for intrathecal and epidural anaesthesia. MECHANISM OF ACTION OF DEXMEDETOMIDINE Dexmedetomidine is the dextro enantiomer of medetomidine, the methylated derivative of etomidine, its specificity for the alpha-2 receptor is 8times that of clonidine, with an alpha-2/alpha-1 binding affinity ratio of 1620:1 & its effects are dose dependently reversed by
The total serum protein concentration (C) was calculated as follows: C (mg/dl) = A sample × concentration of the standard A standard. 184.108.40.206 Albumin Serum albumin was determined using Bromocresol Green (BCG) method (Peter et al 1982). Measurement of serum albumin is based on its quantitative binding to the indicator 5, 5-dicromo-o-cresolsulphonaphthaline (bromocresol green, BCG). Non-haemoloysed serum was mixed with a buffered BCG reagent and incubated at 20-25oC for 5minutes. The absorbance of the sample and that of the standard were measured against the reagent blank at a wavelength of 630nm and albumin was calculated as follows: g/l = Δ A sample / Δ A standard × standard concentration (6%) (Where A = absorbance).
Abstract In this experiment, the reaction kinetics of the hydrolysis of t-butyl chloride, (CH3)3CCl, was studied. The experiment was to determine the rate constant of the reaction, as well as the effects of solvent composition on the rate of reaction. A 50/50 V/V isopropanol/water solvent mixture was prepared and 1cm3 of (CH3)3CCl was added. At specific instances, aliquots of the reaction mixture were withdrawn and quenched with acetone. In addition, phenolphthalein was added as an indicator.
Seconal Sodium (Secobarbital Sodium) Classification: Barbiturates (Broad) Molecular Weight: 260.27 g/mol pKa: 7.8 Molecular Formula: C12H17N2NaO3 IUPAC Name: 5-pentan-2-yl-5-prop-2-enyl-1, 3-diazinane-2, 4,6-trione Description Secobarbital is a barbiturate, which is a nonselective CNS depressant, they are often used as sedatives. Secobarbital was at one point used for induction of anesthesia before other general anesthetics agents became commonly used. The drug has also been prescribed for short-term treatment of insomnia on rare occasions. Secobarbital is a CII schedule drug, substances in this schedule have an elevated potential to be abused. Secobarbital is orally administered, 100 mg as a sedative and 200 to 300mg 1 to 2 hours
The UV sensitive bands were purified using repetitive preparative TLC followed by crystallization. The identity of Ecdysterone was established by the following procedure: HPLC, with a Shimadzu LC-20, a Phenomenex C-18 reverse-phase Luna C18 which was used with a mobile phase of MeOH:Water (1:1) at 1.80 mL/min and the absorbance was monitored at 254 nm. Studies confirming the presence of a single peak of the isolated Ecdysterone, with a characteristic UV absorption at 246 nm were done using commercial standard Ecdysterone (Sigma) (Figure 2 A and B).
Typically, due to the dual reactivity of organosilane, both alkoxy group (OR\) and non-hydrolysable organic moiety (X), it is used to couple inorganic or organic substrates such as minerals, metals or cellulose and polymeric matrices such as rubber, thermoplastic or thermosets to improve its physical properties. The applications include adhesion promoter, dispersing/hydrophobing agent and crosslinking agent. 1.3.1 Adhesion Promoter Organosilanes are effective adhesion promoters when used as integral additives or primers for paints, inks, coatings, adhesives and sealants. As integral additives, they must migrate to the interface between
Because it is a tertiary benzylic halide, the reaction is considered an SN1 type. To test the purity, the class then uses a TLC. When one places,” a spot of the substance on the absorbent surface of the TLC plate, the solvent (or solvents) run up through the absorbent,” (Zubrick223). The initial mass of the reactant, triphenylmethyl chloride was 2.006 grams. The experiment yield is 1.589g, which is a 80.3% yield.
Isolation of Ibuprofen: Fifteen 200 mg ibuprofen tablets were used, which was 3.37g (16.34 mmol, 112.3%) ibuprofen. This amount of ibuprofen was submerged in 25 mL of acetone and stirred vigorously for 5 minutes to dissolve. Red coatings of the tablet were separated and vacuum filtration was utilized to dissolve all insoluble components. The melting point range of the crude ibuprofen was 72.4-73.9°C. The major bands from the IR are, FTIR: sp3 O-H stretch, about 3200-2500 cm-1 (b, m); sp3 C-H stretch, 2991-2868 cm-1 (sh, m); sp2 =C-H stretch, 3100-3000 cm-1 (sh, w); sp2 C=O stretch, 1701 cm-1 (sh, s); and sp2-sp2, aromatic, C=C stretch (in ring), 1507 cm-1 (s, m).