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Liquid Chromatography Essay

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Miceller Liquid Chromatography, Chiral Separation Of Drugs A New Horizon For Green Chemistry Abstarct: Inspite of the fact that the use of miceller liquid chromatography(MLC) seen a decline as a separation technique since its inception as an alternative to the regular RPLC, newer and more sophisticated methods are being developed to exploit its green nature of analysis. It was soon evident that pure micellar solutions yield reduced efficiencies. The problem was partially solved with a small addition of an organic modifies(organic sovent).Nevertheless, the wide-ranging difficulty of the poor peak shape persisting as a setback for MLC, despite the fact that the mix mode often offers similar or even improved efficiencies for basic ompounds as …show more content…

The redominant term influencingband broadening in MLC appears to be stationary phase masstransfer. Organic solvents produce a thinner surfactant layer onthe column, which permits better solute diffusion. Anionic surfactantssuppress the silanol effect for basic compounds due to theprotecting coating of the stationary phase by the surfactant, whichseems to be more effective than the direct electrostatic interactionof amines with free silanols.” Introduction: MLC is the result of two articles published on miceller mobile phase by Armstrong in 1977 and 1979. Afterwards the new micelle based mobile phase was used in HPLC the works of Armstrong,Berthod, Cline-Love, Dorsey, Fendler, Hinze, Mullins, Pramauro, Pelizzetti and Sybsilska [1,2] was a driving force for the new for the new technique which we know today as MLC. After a decline in use for a decade it again emerged as an important separation technique, that happed due to the work of Spanish research groups that established its worth in modern pharmaceutical analysis. MLC is an extension of reversed-phase liquid chromatography (RPLC), in which the mobile phases are aqueous

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