Microbe 3c Lab Report Procedure
Analysis of Major Unknown 3C Summary: A series of tests was done with Microbe 3C. A Gram stain was done and the sample was gram positive. A streak plate was completed revealing that the microbe was clear/white in color, margin was entire, shape was circular, elevation was convex/raised, and the opacity was translucent. The last test done was the sucrose test. It starts orange and turns yellow positive for microbe 3C Klebsiella oxytoca (Professor Brady, Personal Communication). Procedure 1 Gram stain: Purpose/ Introduction: The Gram stain reveals whether a microbe is gram positive or gram negative. It also reveals the shape and arrangement of the microbe (Professor Brady, Personal Communication). Of the forty-three possible microbes the following are gram positive: Bacillus cereus, Bacillus circulans, Bacillus coagulans, Bacillus megaterium, Bacillus subtilis, …show more content…
The following was eliminated because they are gram positive: Bacillus cereus, Bacillus circulans, Bacillus coagulans, Bacillus megaterium, Bacillus subtilis, Bacillus thuringiensis, Brevibacillus brevis, Geobacillus stereothermophilus, Lysinibacillus sphaericus, Clostridium perfringens, Corynebacterium pseudodiphtheriticum, Listeria monocytogenes, Lactobacillus casei, Enterococcus faecalis (Streptococcus), Streptococcus lactis (Lactococcus), Streptococcus mutans, Streptococcus pneumonia, Micrococcus (Kocuria) roseus, Sarcina lutea (Micrococcus luteus), Sporosarcina ureae, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, and Rhodococcus rhodochorous. The remaining microbes are gram negative: Alcaligenes faecalis, Rhizobium radiobacter, Salmonella typi, Serratia marcescens, Shigella flexneri, Yersina pseudotuberculosis, Erwina persicinus, Serratia liquifacens, Halobacter salinarium, Enterobacter aerogenes, Eschirichia coli, Flavobacter capsulatum,
Unknown Microbial # 398
532 Words | 3 PagesMethods Unknown microbial #398 went through several of tests in order to identify its characteristics when isolated from a urine sample of Doris, a 64- year old patient with a kidney infection. To identify unknown #398, must prepare a working and a reserve stock by the inoculation from a broth culture and by quadrant streaking method on a PEM and EMP plates. The following test procedures were incubated at 37°C for 48 hours for observation and identification for unknown #398. The identification of unknown #398 followed test procedures from Brown1.
Bio 655 Week 1 Lab Report
1378 Words | 6 PagesDr. Colleen Winters – BIO 655 Vishall G. Kaistha TITLE: “Recombination-Directed DNA Repair Promote Homologous Stimulating Transcription of Genes That That Preserves Genomic Integrity by MEN1 Is a Melanoma Tumor Suppressor”.
Unknown Culture Lab Report
1054 Words | 5 PagesIdentification of bacteria within Unknown Culture #21 In this experiment, an unknown culture of two different types of bacteria was assigned to each person, a number of tests were performed to isolate and identify these bacterial cells. Based on knowledge from the previous experiments completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test.
Hsc300 Unit 9 Lab Report
313 Words | 2 PagesMedical biller is a position that will require you to take in medical claims and code them and bill out medical claims to insurance companies, Medicare and Medicaid on a daily basis. You will have to reconcile Explanation of Benefits (EOB) weekly. Verify if insurance companies require that patients get PA for certain procedure and products. Five requirements for Medical Biller position 1. How to bill claims 2.
Lab Report Identifying Unknown Microorganisms
1596 Words | 7 PagesIn this experiment, we cultivated an unknown specimen containing two microorganisms. The purpose of this experiment was to use a variety of biochemical test previously learned in the lab to identify the unknown bacteria. The identification of unknown bacteria is a major part of microbiology. Microbiologist observe samples such as blood and sputum in the laboratory for the presence of microorganisms. Identifying unknown bacteria is extremely important in clinical settings because it helps physicians find treatment for infections.
Blast Off Lab Report
407 Words | 2 PagesIn the “Blast Off” lab, we had launched a foam rocket into the air by pumping air into a nozzle, shooting the rocket up, and then recording the time from launch to when it hit the ground. I have learned and now understand the mechanics of kinetic and potential energy. The experiment I had conducted relates to energy in that as we observed the rocket, its energy was constantly transforming as it was in motion. Kinetic energy is an object’s energy based on its motion. Potential energy is energy based on an object’s shape or position.
Examples Of Unknown Lab Report
694 Words | 3 PagesUnknown Paper I Introduction This lab is a presentation of lab tests performed to finalize a conclusion based on results to identify the given unknown bacteria. The unknown bacteria was identified based on lab test results in the table provided in class for the possible unknown bacteria. The unknown bacteria identified as #36, and based on the lab tests is Enterobacter II Materials and Methods Catalase Test- this test determines whether bacteria have the enzyme catalase which catalyzes the breaks down hydrogen peroxide.
Unknown Lab Report Essay
408 Words | 2 PagesThe unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not
Bio 13 Lab Report
1009 Words | 5 PagesIn this three-week long experiment conducted in the Bio 13 Lab, we were able to analyze a single nucleotide polymorphism (SNP) in our own genomic DNA and then determine our genotype at this specific SNP. In week one, we extracted genomic DNA from our cheek cells with swabs and prepared our DNA for PCR (Polymerase Chain Reaction) that would amplify the region with the intended SNP of interest. After one week and after the PCR was run outside of the lab section, the resulting PCR product was purified and treated with restriction enzyme Ahdl in order to prepare for the final analysis of our genotypes. In the third and final week of the project, we analyzed our PCR products by means of agarose gel electrophoresis. By the conclusion of the experiment, we had completed the analysis at the SNP of interest and determined our genotypes for this SNP.
Alcaligenes Faecalis Experiment
636 Words | 3 PagesAccording to the series of test that my group ran for our unknown specimen, we had a match with the bacteria known as Alcaligenes Faecalis. This bacterium belongs to one of the major group of gram-negative bacteria (Phylum Proteobacteria). Alcaligenes Faecalis (Genus, species) is a rod shaped (bacillus), 0.5-1.2 x 1.0-3.0 µm, round with scalloped margin (colony configuration growth), motile (with one to nine peritrichous flagella), gram-negative, non-fermentative bacteria, obligate aerobic, having oxygen as the principal terminal electron acceptor in the electron transport chain (ETC). We consider we have a match with the species Alcaligenes Faecalis because of the following reasons: Fermentation tests performed (Durham sugars) were negative, which indicate that our bacteria use a different metabolic means for growth (non-fermentative gram-negative bacteria).
Exercise 14 Unknown Identification Lab Report
449 Words | 2 PagesThe second ½ of the organism was used for gram staining. The gram stain method was performed on the unknown organism per lab manual page 42 and two gram stain reactions were identified. Organism B was gram positive cocci in grape like clusters. Because organism B was positive I could eliminate Escherichia coli, Enterobacter aerogenes and Proteus vulgaris because these bacteria would be rod shapes. Organism A was gram negative pink rod shaped and because of gram positive morphology I could eliminate Staphylococcus aureus, Streptococcus lactis and Bacillus subtilis.
Chronic Periodontitis Literature Review
1159 Words | 5 PagesThey appeared to be a group of bacteria that existed in the biofilm milieu, less cognitively associated with other individual bacterial species. The orange cluster consisted of Fusobacterium species, Prevotellaspecies, Micromonasmicros, Peptostreptococcus micros, Campylobacter species, Eubacteriumspecies and Streptococcus constellatus. These species have been considered bridging species related to both, their physiological capabilities to use and release nutrient substances in the biofilms, and the recognition that they express cell surface structures and can bind to the early colonizers and to members of the red complex.
Glucouronidase Test Essay
916 Words | 4 PagesFollowing are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Beta glucouronidase test is used for the identification of Escherichia coli. An enzyme is produced by E.coli which is beta D glucouronidase. Beta d glucouronidase in turn hydrolyzes beta d glucopyranosid uronic derivatives to aglycons and D glucuronic acid. Bile solubility test is used in laboratory for differentiation of alpha hemolytic Streptococci from Streptococcus pneumoniae.
Mastitis Research Paper
713 Words | 3 Pages, Klebsiella sp. , Enterobacter sp. Acinetobacter sp. , Actinobacillus sp., Vibrio sp., Micrococcus species, Lactococcus lactis ssp lactis, Yersinia sp. and Neisseria sp.
More about Microbe 3c Lab Report Procedure
Unknown Microbial # 398
532 Words | 3 PagesBio 655 Week 1 Lab Report
1378 Words | 6 PagesUnknown Culture Lab Report
1054 Words | 5 PagesHsc300 Unit 9 Lab Report
313 Words | 2 PagesLab Report Identifying Unknown Microorganisms
1596 Words | 7 PagesBlast Off Lab Report
407 Words | 2 PagesExamples Of Unknown Lab Report
694 Words | 3 PagesUnknown Lab Report Essay
408 Words | 2 PagesBio 13 Lab Report
1009 Words | 5 PagesAlcaligenes Faecalis Experiment
636 Words | 3 PagesExercise 14 Unknown Identification Lab Report
449 Words | 2 PagesChronic Periodontitis Literature Review
1159 Words | 5 PagesGlucouronidase Test Essay
916 Words | 4 PagesCoccus Pathogens
126 Words | 1 PagesMastitis Research Paper
713 Words | 3 Pages