Exercise 14: Unknown Identification Lab Report The purpose of the study was to identify the unknown bacterium using various biochemical tests in addition to using scientific methods in determining the outcome of the hypothesis. Each biochemical test will help determine the bacteria based on specific characteristics of each organism. I was giving unknown number 232. The first procedure that needed to be done after obtaining unknown bacterial mixture was to isolate the two bacteria in a pure culture using the streak plate method described in Microbiology Laboratory Manual Eight Edition. The material used was trypticase soy agar (TSA) plate, nutrient plate, starch agar, hydrogen peroxide, iodine reagent and microscope.
The unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not
Being able to identify unknown microbes from systematic testing is what makes the field of microbiology so important, especially in infectious disease control. Using the testing procedure laid out by the microbiology field we are able to identify unknown bacteria present in our everyday lives, and along the way learn a lot about their characteristics that separate them from other types of bacteria. Being able to do this is vital in order for us to understand why microbes are present in certain places, how they are able to grow and what restricts their growth, that way they can be combatted if necessary. These techniques for determining unknowns are also important for isolating and testing infectious disease microbes in order to prevent spreading. Another important aspect of being able to identify unknown microbes is the
Due October 19th, 2015 Erin Gibbs: 200270053 Dr. Andrew Cameron Bio 222 Written Assignment 1 1. Health officials realized that the mysterious illness was not caused by a bacterium because the pathogen would be identified quickly in comparison to a virus, which is more complicated (CBC, 2013). 2. It was hard to identify the infectious agent because SARS presented as flu-like symptoms with a rapid onset and is similar to the common flu (CBC, 2013). People who had other underlying health problems made it difficult to identify symptoms of SARS because it made it hard to differentiate (CDC, 2013).
Answer these questions in their entirety. For each of these questions, you should do some research and cite the sources you’ve found to support your position You may use the book as one source. Bring the book and your notes to class. Look for other sources by researching academic articles, newspaper articles, magazine articles, excerpts from books, etc. to support your answers.
The effects of alcohol on Biological Membranes. Introduction In this experiment it will be analysed the damage alcohols can have on biological membranes. Membranes are made up of lipids and proteins. Membranes usually help maintain the balance in a cell as it holds all the cellular materials.
Introduction The purpose of this lab is to use control variables to help identify different macromolecules. Biological systems are made up of these four major macromolecules: carbohydrates, lipids, proteins and nucleic acids. Carbohydrates are sugar molecules (monosaccharides, disaccharides, and polysaccharides) which make them the most abundant macromolecule on the earth. Lipids (oils and fats, phospholipids and steroids) are insoluble in water and perform many functions such as energy source, essential nutrients, hormones and insulators (Lehman, 1955).
Indeed, according to the World Health Organisation, water pollution has been defined by the addition of any alien material from either natural or other sources into a water body, thereby changing the natural qualities of water and making it unusable for its intended purpose which could be domestic use, recreational use, etc. Therefore, since only < 1% of the world’s fresh water is accessible for direct human use, when this freshwater becomes polluted, measures must be put in place to cleanse the water from the majority of its impurities. Thus, one method that is commonly used to remove effluents from waterways are sewage treatment plants which are known as centralized systems simply because it is usually located in the central area of a main town or city (Globalchange 2006) A sewage treatment plant may be defined by an industrial structure that treats water that has been used for domestic and other purposes and then discarded. Furthermore, sewage contains a wide variety of contaminants, both chemical and biological and, if not treated, poses a threat to health; hence the industrial structure removes up to 90% of the biological and chemical waste products from the water thus
Biochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining.
The media used in this experiment was Trypticase nitrate broth. The reagents used (A and B) were sulfanilic acid and alpha-naphthylamine (respectively). Using aseptic technique, the bacterium (16A and 16B) were inoculated into labeled broth test tubes. The tubes were incubated for 48 hours at 37 degrees Celsius. When the incubation was complete 5 drops of reagent A and 5 drops of reagent B were added to each of the broths.
Chlorine is accurately put so as to prevent active chlorine remaining at the opening of the plant. Advantages And Disadvantages I. Advantages: - Desalination plants can provide clean drinkable water in areas where no natural supply of drinkable water exists. II. Advantages: - water turns of safe to drink and it is not dangerous or isn’t hazardous to any living thing. III.
Introduction By the year 2025, the Philippines is believed to be brought to a water crisis if no new sources of water are developed or found to meet the growing demand of the citizens. (Sapnu, R., Balabo, D., & Cervantes, D., 2007) "There is a Japan International Cooperation Agency study that says that if we will not conserve water, there may be some problems. But of course we can always start conserving now and look for other sources," Lopez (2013) said. Studies have shown how water conservation and appropriate water utilization are significant as early as today.
My knowledges of molecular biology, biochemistry and microbiology have grown, but I know there is a lot to learn and, as always, to keep learning is my final goal. This is the moment to challenging myself and pursuit other experiences in an area that involve Biochemistry and Molecular Biology. I want to continue my education at the Graduate Program in Biochemistry and Cell Biology at Rice University. This program is the best option to continue improving my scientific and professional skills. The privileged research conditions of your university, the facilities, the faculty and the outstanding researcher lines make this university the perfect place for a doctoral training.