Microemulsion Lab Report

6. CHARACTERIZATION AND EVALUATION OF PROPOFOL MICROEMULSION
6.1CHARACTERIZATION OF MICROEMULSION
6.1.1 PARTICLE SIZE
The particle size characterisation of microemulsion was performed to obtain information about the main particle size of the systems, particle size distribution and about changes over time [54-55]. Particle size is the most important characteristics of microemulsion system. It influences the drug loading, drug release, and stability of microemulsion. Drug release is affected by particle size. Smaller particles have larger surface area, Therefore, most of the drug associated would be at or near the particle surface, leading to faster drug release. Whereas larger particles have large cores, which allow more drug to be encapsulated

Brownian motion is the random thermal, translational, and rotational motion (diffusion) of the particles in the solution. When these particles come in the path of laser light, they scatter the light. Because of the constant motion of the particles, there is a temporal variation in the intensity of the scattered light. These variations are recorded and detected by the detectors in the form of intensity versus time profile. The autocorrelator of the instrument creates a correlation function, which is an exponentially decaying time function. The decay constant of this function is related to the diffusion coefficient in the Stokes-Einstein equation (Equation

Detection : 275nm
Flow rate : 2ml/min
Injection volume : 20µl
Column Temperature: 25oC
Run Time : 25min

Mobile phase preparation
It is prepared by mixing 800ml of Tetrahydrfuran and 1200ml of water mixed and filtered. Preparation of Diluent It is prepared by diluting 68ml of water with Isopropyl Alcohol to 1000ml Preparation of Standard stock solution
Weighed 20.95mg of propofol standard and transferred to 25ml of volumetric flask. Add 10ml of diluent, sonicate to dissolve and make up the volume with diluent. Preparation of Sample preparation
Transfer 2ml of sample solution into 25ml of volumetric flask. Add 10ml of diluent, sonicate to dissolve and make up the volume with diluent
Procedure:
Inject blank(inj)(diluent) and standard preparation(5inj) Check for the following system suitability parameters The relative std deviation for replication inj is not more than 1.5% If the system suitability parameters pass, then inject Assay preparation(2inj) and report the chromatograms

CALCULATIONS:
Calculate the quantity in % of propofol in each ml of inj taken by the formulation.
Qty of Propofol in % = AT/AS × WS/DS × DT/V × 100/LC