Mip Lab Report

Synthesis of MIP by Precipitation Polymerization

The MIPs and NIP are prepared by the non-covalent approach. The molar ratio for the template, functional monomer and cross-linker are 0.5:2:10 respectively. For the preparation of Caffeic acid imprinted polymer (0.5 mmol, 0.09 g) as a template molecule was dissolved in 75 acetonitrile (ACN) in a 150ml conical flask. Styrene as a functional monomer (2mmol, 230µl), (10 mmol, 1.9 ml) of the EGDMA as a cross linker agent and the 0.03g of initiator, AIBN were then added to the solution. The mixture was sonicated for 5 minutes and then followed by a degassed and purged process with nitrogen gas for 15 minutes in the ice bath. The opening of the conical flask was sealed with the aluminium foil and paraffin to prevent oxygen from entering the flask along with the heating process. The conical flask was then emerged in a water bath for 12 hours. The temperature is monitored to be maintained at 60oC for 8 hours to allow the initiation of the reaction and increased to 85oC for the remaining 4 hours. After drying overnight, a rigid structure of the light brown polymer was obtained.

The synthesis of MIP was replicated twice according to the

For dynamic adsorption test, a series of 150 ml conical flasks that contained 0.5 g of the MIP (MIP1, MIP2 and MIP3) and NIP were added in an 80 ml stock solution (50 ppm) of acetonitrile containing 0.1 g of caffeic acid. The flasks were shaken on the shaker at 250 rpm and the samples were collected at different time intervals (60, 120,180,240, 300 and 360 min). A blank sample was prepared by using only the caffeic acid solution to account for any potential of caffeic acid onto the centrifuge tube. The samples MIPs and NIP collected were centrifuged at 4000 rpm for 10 minutes to remove suspended particles for further