Max Pertuz: The Basics Of Molecular Biology
Molecular biology is the branch of biology that deals with the molecular basis of biological activity. This field overlaps with the other areas of biology and chemistry, particularly genetics and biochemistry. Molecular biology chiefly concern itself with understanding the interaction between the various system of the cell ,including the interactions between types of DNA, RNA and protein biosynthesis as well as learning how these interaction are regulated.
The study of physical, chemical and biological macromolecules is termed as Molecular Biology. Max Pertuz is known as the father of molecular biology. The basics of Molecular biology is the DNA which is being used for following application-
-Genetic engineering
-DNA profiling.
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They perform a vast and different type of functions in living organisms, which also include important functions like catalyzing metabolic reactions, replicating DNA, responding to different stimuli, and also transportation of molecules from one location to another within a cell or from one cell to different cell. Proteins differ from one another basically in their amino acids sequence, which is generally dependent on the nucleotide sequence of their genes, and which in turns results in folding of the protein into a specific 3D structure that determines its functions.
A linear chain of amino acid is called a polypeptide and a protein contains generally more than one long polypeptide.
Why do we study proteins? The study of proteins and their function important for understanding basic concepts of cells and organisms. Proteins are important in biology as they are:
• are Biocatalysts that help in maintaining the metabolic processes that are happening in the cell,
• They form the structural elements that are found inside and outside of the
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And at last Sodium acetate is added to remove RNA impurities. The pellet of DNA is obtained which is dissolved in TE buffer and from this buffer DNA can be isolated and also we can go for Agarose gel electrophoresis for Visualization of
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