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Moringa Oleifera Leaves Case Study

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4.1 Extraction and Fractionation To determine the antimicrobial activity of Moringa oleifera , the finely grinded leave part of 500g was extracted using solvent extraction resulting in181.506g . Two different organic solvents were used in decreasing order of polarity to extract the crude fraction. 80g of dried crude is dissolved back in methanol for further extraction. The initial weights of petri plates before occupied with the fractions of extract and after the fractions completely dried were measured repeatedly. The mean value is recorded and used for further calculation. The percentage yield of each solvent extracts of M. oleifera leaves obtained was shown in table 4.1.
Table 4.1: Percentage yield of crude extracts of M. oleifera leaves …show more content…

The results are in accordance with the previous study conducted by Oluduro, 2012. He stated that the aqueous, etanolic, and methanolic extract of M.oleifera leaves did inhibit the E.coli, S.aureous and P.aeruginosa. In another study, Vinoth et al, 2012 has stated that the chloroform fraction of M.oleifera plant extracts only inhibit the E.coli and it was found to be non-active against P.aeroginosa and S.aureus. While the present study suggest that basically the chloroform and hexane fraction of the M.oleifera leaves extract shows inhibition of all S.aureus, E.coli, P.aeroginosa and E.faecalis. The zone of inhibition of chloroform and hexane typically ranges from 10mm to 14 mm for 1000ppm concentration. This result also in accordance with Pal et al, 1995. The range of inhibition for both hexane and chloroform fractions are nearly the same for all type of bacterial strains at same concentration as shown in the figure 4.2. This is maybe due to the presence of same active principle that found in both

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