Fluid Extraction Lab Report

Extraction of gum from Basil seeds

Mucilage was extracted using distilled water. 100 gms of Basil seeds were added to a specific proportion of distilled water at a desired temperature for 2 hours. The soaked seeds were blended for entire extraction period. Slurry was maintained at a constant temperature and continuously stirred using a magnetic stirrer. Later, mucilage was separated from seeds using a rubber spatula on a mesh screen. Slurry obtained was passed through a screen of 8 folds of muslin cloth. Separated mucilage was precipitated with equal amount of acetone. The precipitated mucilage was then separated and dried at 50°C for 10 h in a conventional hot air oven. Also, the adhered mucilage from the dried seeds was separated by rubbing

Standard curve of Moxifloxacin HCL

A calibration curve of Moxifloxacin HCL 2-10 mcg/ml of the drug in 1% v/v acetic acid in distilled water was constructed at a λmax of 288nm using UV-VIS spectrophotometer (Shimadzu Corporation). Beer’s law obeyed to construct the calibration curve in the concentration range of 2-10mcg/ml. Analyses were done in triplicate. The calculation of the drug release rate studies are based on the standard curve11,12,13,14.

PREFORMULATION STUDIES

Prior to the development of the dosage form preformulation studies were carried out on parameters like melting point, Loss on drying, partition coefficient, viscosity of polymers, interference of polymers, interaction studies of drug and

Moxifloxacin was incorporated in 100 ml of Basil seed gum/chitosan/Ethyl cellulose solution and vortexed for 15 min. The viscous dispersion was kept aside for 30 min for complete expulsion of air bubbles. Films were cast by pouring the drug-polymer solution into the center of glass moulds and allowed to dry at room temperature. The dry films were cut into strips of (5mm × 5 mm), wrapped in aluminum foil and stored in a calcium chloride desiccator at room temperature pending