Sample preparation Maize (PMH-1) and chickpea (GPF-2) were procured from Department of Plant Breeding, Punjab Agricultural University, Ludhiana, India. Whole grains of maize and chickpea were ground to pass through 200 μ sieve using laboratory-scale super mill (type 3303, Perten Instruments AB, Sweden). Maize and chickpea flour mixture in the ratio of 80:20 was used as this combination gave the best sensory score (hedonic score 8.1) on the basis of preliminary trials. The flour was blended for 15 minutes to ensure uniform mixing. Proximate analysis Proximate analyses of raw materials i.e.
After incubation period the embryos are allowed to germinate and sown to produce T1 seeds. These seeds are then ground and protein concentrations isolated by using an assay. Then a dilute series of recombinant LT-B and non-transgenic corn included in the assay, same is done with the TGEV. Production of the transgenic grain is then achieved by backcrossing lines of T1 seeds with commercial
Kuar and Sandhu (2014) showed the shoot multiplication rates were ranged from 4 to 25 fold in CoPb 91 and CoJ 83 cultivars, respectively and the complete plantlets were produced in 157 days with 97 percent survival rate. The fidelity of this protocol for agri-business industry was tested by producing approximately 0.1 million saleable HTM sugarcane plantlets in a small-scale (150 m2) tissue culture
Total of 300 one-week old un-sexed Arbor-Acres broiler chicks were distributed into 10 treatments. Birds were fed ten experimental diets in which the first was the control group (T1) a corn soybean diet according to NRC requirement and the other groups (T2,T5 and T8) were formulated in which the BBP was incorporated at levels from 25 - 75% in increments of 25% (adjusted to NRC energy requirements),while groups (T3,T6 and T9) were formulated as the same of groups T2,T5 and T8 without adjusted to NRC energy requirements, while groups (T4,T7 and T10) were formulated as the same of groups T3,T6 and T9 by adding 0.05 % Xylam enzyme (mixture of B-xylanase and α-amylase). All diets (starter and finisher) were formulated to be iso-nitrogenous and given to birds from the end of the first week until 28 days (starter diets) and from 29 to 42 days of age (finisher diets).The results of this study showed that no significant differences were observed between treatments in LBW at 7 days of age, however the only significant difference in LBW (P< 0.01), but the enzyme influence depended to growth stage. In general, both experimental diets type and enzyme supplementation had a highly significant (P < 0.01) effects on average daily gain in all experimental periods. The trial diets sort
EVALUATION TEST FOR LOZENGES Quality Control (52) Candy Base- For the candy base it is essential to check for corn syrup and sugar delivery gears; temperature, steam pressure, cooking speed, temperature and vacuum of candy based cooker. MoistureAnalysis Gravimetric method: Weigh 1g of sample and noted as its initial weight, it is then placed in a vacuum oven at 60-70oC for 12-16 hours. After specified intervals of time, once again weigh the sample and moisture content can be calculated using the following formula. Moisture Content =Initial weight – final weight Karl Fisher titration- A sample of the prepared lozenge is calculated to obtain 10-250mg of water which is then titrated with Karl Fischer reagent. Azeotropic distillation method
Plant material and extraction 300gm dried powder of seed was weighed & taken in a aspirator (2.5L). Before placing powders into the aspirator, the jar was washed properly with acetone and then dried. 800ml of solvent i.e. methanol was added gradually. The container with its content was sealed & kept for 20 days with occasional shaking & stirring.
Isolation of Streptomyces sp The soil samples were collected from various agricultural areas which had been serially diluted up to 10-5. The spread plate technique was followed and incubated at 28°C for 7 days. The Streptomyces sp isolates were maintained on starch casein agar slants. Screening for antimicrobial activity by well diffusion method The Streptomyces isolates were grown in starch casein broth at 28ºC for 10 days and the activity were observed by well diffusion method. The culture filtrate was loaded into the wells which had been inoculated with test organism, S.aureus, K.pneumonia, P.aeruginosa and E.coli and incubated for overnight and measured the zone of inhibition.
of termites dead Per cent Mortality = ------------------------------------------- X 100 Total number of termites released Evaluation of antifeedant activity of plant extracts Cardboard sheets weighing 2.5g were taken and moistened with distilled water and shade dried for one hour. Then the sheets were thoroughly immersed in the oil solutions and kept aside for drying. Chlorpyrifos 20EC @ 0.1 per cent solution was the standard check. The treated sheets were placed in the Petri dishes and 50 numbers of workers/ soldiers were released and covered. This set up kept in the BOD incubator at 25±1º C for 48 hours.
Mechanical pre-treatment such as grinding was found to have an important contribution on morphine reduction (Sproll, 2007). In the toxicological study by the Bavarian State Office for health and food safety and Federal Institute for Risk Assessment (Bfr) derived a provisional guidance value of 4mg/kg. The influences of food processing found a significant reduction of morphine during steps like grinding and baking. Poppy seed for decorative purposes (e.g. topping and buns) could contain upto 100mg/kg of morphine which was far off from the tolerable daily intake (Sproll,
The study population of this study was the agricultural workers who were using pesticide for their crops cultivation. For comparison the health problems attributable to pesticide toxicity, the study populations were selected from the Integrated Pest Management (IPM) and Non-Integrated Pest Management (Non-IPM) areas of Gazipur district in Bangladesh. The data were collected from the agricultural workers of both the areas who were found to work in the field for cultivation. During data collection period the agricultural workers who were available in field and agreed to participate in the study and having experience of pesticides spray for at least one year were selected as the respondents. Accordingly a total of 180 agriculture workers irrespective of age were included as respondents of this study.