Mycophenolate Moofetil Lab Report

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RESULTS & DISCUSSIONS
PREFORMULATION:
S.NO API CHARACTERISATION RESULTS
1 Physical Appearance Mycophenolate mofetil white to off-white crystalline powder
2 Melting point 93-94ºC
3 solubility Freely soluble in acetone, soluble in methanol, and sparingly soluble in ethanol.

Table 3: Characterization of API
Calibration Curve of Mycophenolate mofetil:
Calibration Curve of Mycophenolate mofetil in pH 7.4 PBS
Solvent pH 7.4 phosphate buffer Saline (PBS)
Wave length 253 nm
Unit for concentration mcg/mL
Table 4: parameters for Calibration curve

Table 5: Calibration data of Mycophenolate mofetil in pH 7.4 PBS
S.NO Concentration (mcg/mL) Absorbance
1 0 0
2 2 0.185
3 4 0.345
4 6 0.526
5 8 0.722
6 10 0.907
Calibration Curve of Mycophenolate mofetil:
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The particle size was found to be in the nanometer range of 260nm- 632nm (figure 10). The characteristics of the MNP prepared with different concentrations of chitosan were studied. The results indicated that the particle size increased with increasing the concentration of CS. The formation of nanoparticles was only possible for some specific concentrations of CS. This fact was also verified in our study that in order to avoid the formation of any micro-particles, the concentration of chitosan needed to be below 1.0 mg/mL. In these concentration ranges, it seemed that the concentration of chitosan had little effect on the mono dispersity of the nanoparticles. It is known that under acidic conditions, there is electrostatic repulsion between CS molecules due to the protonated amino groups of CS; meanwhile, there also exist inter chain hydrogen bonding interactions between CS molecules. In low concentration of chitosan the intermolecular hydrogen bonding attraction and the intermolecular electrostatic repulsion are in equilibrium. Therefore chitosan concentration increases (0.05 - 0.7 mg/ml), chitosan molecules approach each other with a limit, leading to a limited increase in intermolecular cross-linking and thus larger but still nanoscale particles are formed. Above this concentration, microparticles are easily formed probably due to the stronger…show more content…
Regression coefficient and slope values are calculated and interpreted. The regression coefficient value of this plot was found to be 0.984 and the slope was found to be 0.699 (figure 17). Figure 17: Koresmeyer plot for Optimized formula
Hixson Crowell Model: In this model, graph is plotted between Cubic root of % drug remaining Vs time. Regression coefficient and slope values are calculated and interpreted. The regression coefficient value of this plot was found to be 0.985 and the slope was found to be 0.112 (figure 18). Figure 18: Hixson plot for Optimized formula
Stability study:
The stability study was carried using the batch MNP5. The stability of drug loaded nanoparticles was evaluated in terms of its drug content16. The stability of nanoparticles was evaluated in PBS (pH 7.4). Nanoparticles formulation was incubated at 5-8° and 37 ± 1° for a period of 60 days. After specified time intervals, the suspension was centrifuged at 15,000 rpm for 1 h, supernatant was removed and nanoparticles were dissolved in dichloromethane. After adding of water and separation, the amount of drug was detected by UV-Vis spectrophotometrically method at 253

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