Mycoplasma Case Study

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Molecular characterization of Mycoplasma contaminating tissue cultures. Dina, Y,H ,Elshafey*, Abo-shama, U.H. **, Saad, A.A *** * Department of Mycoplasma Research ** Department of Microbiology, Faculty of Veterinary Medicine, Sohag University ***Department of Virology Research– Animal Health Research Institute. ABSTRACT One of the main problems in cell culture unit is mycoplasma contamination. It can extensively affect cell physiology and metabolism. As the applications of cell culture increase in research, industrial production, cell therapy and its importance for diagnostic routine work in selected labs, so more concerns about mycoplasma contamination will arise. In our study, a total of 50 cell cultures from Animal Health Research Institute,…show more content…
The outer layer is instead, a three layered membrane containing sterols. Diameters of these organisms may range from 0.2-0.3 µm and, due to their plasticity, are able to pass through the pores of a 0.2 micron filter with applied pressure. Because the morphology of Mycoplasma is pleomorphic, they occur as two different structural forms during a life cycle: coccidian, a spherical or spheroidal shape, and filamentous, resembling rods. (Davis, 1994). Each batch of live viral vaccine, each lot of master seed virus (MSV), each lot of primary and master cell stock (MCS), and all ingredients of animal origin not steam sterilized should be tested for the absence of mycoplasmas. Solid and liquid media that will support the growth of small numbers of tested organisms such as typical contaminating organisms Acholeplasma laidlawii, Mycoplasma arginini, M. fermentans, M. hyorhinis, M. orale, and M. synoviae should be used. The nutritive properties of the solid medium should be such that no fewer than 100 CFU should occur with each tested organism when approximately 100–200 CFU are inoculated per plate. An appropriate colour change should occur in the liquid media when approximately 20–40 CFU of each tested organism are inoculated. The ability of the culture media to support growth in the presence of product should be validated for each product to be tested, and for each new…show more content…
Mycoplasmas usually adhere to cells but, depending on the species, may fuse with the host cell or even invade it (Balish et al., 2002; Dimitrov, 1993 and Lo et al., 1993). These bacteria deplete the nutrients of cell cultures and interfere with the response of these cells when challenged experimentally(Miyazaki, et al., 1990). Interruption of cell metabolism (Pollack et al., 1997), modulation of the immune response (Chambaud et al., 1999 and D’Orazio et al., 1996), modification of cellular morphology, interference with viral replication, chromosome modifications, or cell transformation may occur (Razin et al., 1998). The identification of these phenomena in accidental or experimental infections may contribute to the understanding of the relationship between mycoplasmas and the host cell (Rottem,

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