Essay On Mycoplasma
Molecular characterization of Mycoplasma contaminating tissue cultures.
Dina, Y,H ,Elshafey*, Abo-shama, U.H. **, Saad, A.A ***
* Department of Mycoplasma Research ** Department of Microbiology, Faculty of Veterinary Medicine, Sohag University ***Department of Virology Research– Animal Health Research Institute.
ABSTRACT
One of the main problems in cell culture unit is mycoplasma contamination. It can extensively affect cell physiology and metabolism. As the applications of cell culture increase in research, industrial production, cell therapy and its importance for diagnostic routine work in selected labs, so more concerns about mycoplasma contamination will arise. In our study, a total of 50 cell cultures from Animal Health Research Institute, Dokki , Giza., VACSERA and Veterinary Serum & Vaccine institute Abbasia were monitored for mycoplasma using culture and PCR methodology. The contamination was detected in the cell culture collected from all
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Mycoplasmas usually adhere to cells but, depending on the species, may fuse with the host cell or even invade it (Balish et al., 2002; Dimitrov, 1993 and Lo et al., 1993). These bacteria deplete the nutrients of cell cultures and interfere with the response of these cells when challenged experimentally(Miyazaki, et al., 1990). Interruption of cell metabolism (Pollack et al., 1997), modulation of the immune response (Chambaud et al., 1999 and D’Orazio et al., 1996), modification of cellular morphology, interference with viral replication, chromosome modifications, or cell transformation may occur (Razin et al., 1998). The identification of these phenomena in accidental or experimental infections may contribute to the understanding of the relationship between mycoplasmas and the host cell (Rottem,
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