Myristica Fragrans Lab Report

Formulation of topical gel containing plant extract [15]

20 g gel was prepared by simple dispersion method. Different concentrations of Carbapol 934 were used to prepare the formulation. Required amount of Carbapol and adequate amount of purified water was taken in a beaker. It was mixed to get a uniform mixture using magnetic stirrer and kept overnight for swelling. 1 ml of the extract and Piperine was dissolved in ethanol and added to the polymer solution along with propylene glycol and methyl paraban with gentle stirring. Few drops of triethanolamine was added and mixed well which result in the formation of Myristica fragrans gel.

Table 1: Composition of topical gel formulation

S. NO

INGREDIENTS

MF1

MF2

MF3

1

Myristica fragrans extract

1) Fig 1: Extract from seeds of Myristica fragrans

Phytochemical screening of Myristica fragrans extract

The ethanolic extract of Myristica fragrans showed positive results for flavonoids and tannins and negative results for carbohydrates, glycosides, proteins and alkaloids.

Identification of Myristicin in the plant extract

Four spots were obtained by TLC and Rf values were calculated to obtain 0.7, 0.53, 0.38, 0.25 from which 0.7 determines the reference value Rf of Myristicin (Fig. 2)

Fig. 2: TLC of Myristica fragrans extract in Toluene: Ethyl acetate (93:7).

Formulated topical Gel

The Myristica fragrans gel was prepared using three different concentration of Carbapol 934 and were further subjected to evaluation of various parameters (Fig. 3).

Fig. 3: Formulated Myristica fragrans gel Table 4: Physical evaluation of all formulations

Parameters

MF1

MF2

MF3

Colour

brownish yellow brownish yellow brownish yellow

Odour

aromatic aromatic aromatic

Clarity

+

++

++

Homogeneity

+

++

+

Spreadability

1.1