Add deionized water to the volumetric flask to the 250ml mark on the volumetric flask. 13. Read the volume from the bottom of the meniscus. 14. Swirl the solution to ensure that the oxalic acid crystals are properly dissolved in the deionised water.
We then slowly added 25ml of chilled deionised water to the filtrate to initiate crystallization by using a measuring cylinder and a dropping pipette, once we had done this we left it for about 10 minutes to allow crystallization at room temperature. We then weighed a filter paper which we will use later in the experiment. We then collected the crystallized acetylsalicylic acid by vacuum filtration in a Buchner funnel and washed the product with a little ice-cold water. We then pre-weighed a clean, empty watch glass and labelled it with our initials and the date, we did this do we could easily identify that it was ours when we go to weigh it with the crystals on. We
ZPFe (3 mol%) was added to a mixture of a benzoyl chloride (10 mmoL) and an aromatic compound (10 mmoL). The reaction mixture was stirred for the appropriate reaction times at 80 °C (Table 2). After completion of the reaction (monitored by thin-layer chromatography, TLC), the mixture was diluted with Et2O and filtered. The organic layer was washed with 10% NaHCO3 solution and then dried over anhydrous Na2SO4. The solvent was evaporated under reduced pressure and the product purified by column chromatography on silica gel to give the corresponding pure aryl
Autoclave the noble agar mixtures to sterilize. These mixtures can be made in advance and stored at 4 °C but should be heated again at the time of the experiment until agar has completely dissolved. 9. Prepare nitroblue tetrazolium chloride solution by making a 1 mg/ml stock solution in 1x PBS (8 g NaCl, 0.2 g KCl, 1.44 g Na2HPO4, and 0.24 g KH2PO4 in H2O to final volume of 1,000 ml). This will be used at the end of the experiment to stain the colonies.
The purpose of the catalase test was used to identify the catalase positive bacteria (staphylococci) and catalase negative bacteria(streptococci). The results were as follows: Staphylococcus aureus (control)- catalase positive Enterococcus faecalis(control)- catalase negative Nostril Microflora on NA- catalase positive Nostril Microflora on PYCa- catalase negative Nostril Microflora on MSA- catalase positive Figure 1. Staphylococcus aureus gram stain control Figure 2. Enterococcus faecalis gram stain control Staphylococcus aureus and Enterococcus faecalis were used as control in both catalase test and gram staining Figure 3. Nostril Microflora on NA plate-gram stain Figure 4.
A number of 11 two ml microcentrifuge tubes were assembled and was labeled as: Tubes 1a-c through 3a-c: Product Extract Dilution 1 through 3 (repeat three times for 9 tubes), Tube 4: Positive control, α-tocopherol and Tube 5: Negative control, solvent only. α-tocopherol, also known as the Vitamin E was prepared as the postive control. A concentration of 10 mg/ml was obtained by the stock solution (in ethanol, it must be kept in the refrigerator and shielded from light).10 µl from the Vitamin E stock was removed and transferred in a labeled eppendorf, at cool temperature and protected from light. 40 µl of 100% ethanol was added to the positive control tube. 50 µl of prepared extract was added at 2 mg/ml to Tube 1 a-c ( 100 µg of overall extract).
The samples were filtered by vacuum over pre-weighed and wetted glass fiber filters (24 mm GF/C; Whatman; Kent, UK). The samples on the filters were rinsed with 5 ml stop solution to remove non-specific, cell surface binding of 3H-FLC. The filters with fungal balls were either allowed to dry for 24-48 hr or were baked in a drying oven for 15 minutes at 95° C. Each dried filter containing fungal balls was then re-weighed to obtain the dry mass of each fungal sample. The filters were finally transferred to 5 mL scintillation vials containing 3 ml of scintillation cocktail (Ecoscint XR, National Diagnostics, Atlanta GA). Radioactivity associated with the fungal sample on each filter was measured in a liquid scintillation analyzer (Beckman Coulter, LS 6500 multipurpose scintillation
The purpose of this experiment is to perform a two step reductive amination using o-vanillin with p-toluidine to synthesize an imine derivative. In this experiment, 0.386 g of o-vanillin and 0.276 g of p-toluidine were mixed into an Erlenmeyer flask. The o-vanillin turned from a green powder to orange layer as it mixed with p-toludine, which was originally a white solid. Ethanol was added as a solvent for this reaction. Sodium borohydride was added in slow portion as the reducing agent, dissolving the precipitate into a yellowish lime solution.
In this lab, two different titrations were performed with three different antacids to determine which brand is the most effective at the cheapest price. The antacids were ground up separately and approximately 0.2 grams of it was placed in a flask. Methyl Orange, an indicator, and a stir bar were added into the flask. The flask was then put on a stir plate which was under a buret with 0.1M hydrochloric acid. The acid was poured into the flask until there was a permanent pink colour.
Gil-Rodríguez et al. (2008) purified the peroxidase from Japanese radish by several purification steps. The juice extracted from roots of radish was ultra-centrifuged using 10 kDa membrane. The retentate was then loaded on Macro-Prep High-S medium at pH 6.1 and eluted with a linear gradient of 0–1 M NaCl. The eluate was further loaded on Macro-Prep High-Q medium