Myristica Fragrans Lab Report

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Formulation of topical gel containing plant extract [15]
20 g gel was prepared by simple dispersion method. Different concentrations of Carbapol 934 were used to prepare the formulation. Required amount of Carbapol and adequate amount of purified water was taken in a beaker. It was mixed to get a uniform mixture using magnetic stirrer and kept overnight for swelling. 1 ml of the extract and Piperine was dissolved in ethanol and added to the polymer solution along with propylene glycol and methyl paraban with gentle stirring. Few drops of triethanolamine was added and mixed well which result in the formation of Myristica fragrans gel.
Table 1: Composition of topical gel formulation
S. NO
INGREDIENTS
MF1
MF2
MF3
1
Myristica fragrans extract
…show more content…
1) Fig 1: Extract from seeds of Myristica fragrans
Phytochemical screening of Myristica fragrans extract
The ethanolic extract of Myristica fragrans showed positive results for flavonoids and tannins and negative results for carbohydrates, glycosides, proteins and alkaloids.
Identification of Myristicin in the plant extract
Four spots were obtained by TLC and Rf values were calculated to obtain 0.7, 0.53, 0.38, 0.25 from which 0.7 determines the reference value Rf of Myristicin (Fig. 2)

Fig. 2: TLC of Myristica fragrans extract in Toluene: Ethyl acetate (93:7).

Formulated topical Gel
The Myristica fragrans gel was prepared using three different concentration of Carbapol 934 and were further subjected to evaluation of various parameters (Fig. 3).

Fig. 3: Formulated Myristica fragrans gel Table 4: Physical evaluation of all formulations
Parameters
MF1
MF2
MF3
Colour
brownish yellow brownish yellow brownish yellow
Odour
aromatic aromatic aromatic
Clarity
+
++
++
Homogeneity
+
++
+
Spreadability
1.1

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