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Mystery Microbiology Test

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Introduction: In the field of microbiology being able to identify a specific bacterial species is an important skill. In order to discover and being able to identify any microbial bacteria, there a list of test one must perform in order to come with the right microorganism. It is fundamental to be aware of the risk of toxify, the resistance to antibiotics and determining how to prevent its growth and being able to destroy this bacteria. By being able to run both physical and chemical test to determine the identity of the mystery microbe is a unique and useful skill in the field of medicine and microbiology. Each …show more content…

After collecting the information, then the top candidates will be eliminating using identification table, and the Lab manual and consulting the results obtained. Comparing and contrasting and go further with results would be essential to predict what the mystery microbe could be. Also further use of the manual plus web resources to make final selection of the Mystery microbe. This decision will be defended with an argument supported with the necessary data from the result table, plus the flow chart. The ultimate objective of this report is to narrow down the possibilities of bacteria using a series of test, while eliminating unlikely choices. Using the skills in the microbiology lab you can discover and make a educated decision of determining the mystery microbe, with the enough supported information collected from the chemical test and stains. There will be 17 test that will be run, each of this test will bring an important clue in determining the Mystery Microbe based on their environmental …show more content…

Nitrate reduction in the gram negative bacteria was conducted and when bacterium produce nitrate reductase when grown in a medium containing nitrate, the enzymes will convert the nitrate to nitrite. If nitrite is present the medium will turn red. This indicates a positive test (Goldman). If the bacterial is one of the few specials that can produce the enzyme Urase, this will be the key test that will separate this gram negative from all of the other possible candidates. Narrowing down the unknown microorganism to gram negative, this approach was helpful to take the next step, in some bacteria the cell wall is surrounded by cell enveloped called capsule, also some bacteria make capsule when faced in a harsh environment to protect them. A capsule stain was preform, the results were analyzed and observed. An additional procedure that was done, was the Fast Actin staining which helps to see if the bacteria contains Mycolic acid in their cell walls, which determines the structure and function of the cytoskeleton in living and fixed cells (Shah). As expected for both E.coli and K. Pnenumia the fast acting results were negative. For both E.coli and K. Pnenumia the Oxidase test was positive a reaction was obtained. The EMB agar is also an important procedure test that helps distinguishing pathogenic microbes in a short period of time (Shah). In the result table it shows the

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