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Digest Starch Lab Report

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Concentration of NaCl verses the time taken to digest starch
Concentration of NaCl (mol)
Time taken for amylase to digest starch (s)
Control (0.0)
110
0.1
116.67
1.0
300
3.0
100
6.0
100

Discussion

The data partially supports the hypothesis of chloride ions increasing the efficiency of amylase. In the 3.0 mol and 6.0 mol, the hypothesis was supported by the average time of digestion taking 100 seconds for both, however the 0.1 mol and 1.0 mol took longer to digest the starch with the 1.0 mol being an anomaly [see figure 1]. The results obtained showed that the higher the concentration of NaCl the faster the the starch will be digested. The results collected were not within a reasonable margin of error to have decisive …show more content…

This experiment showed that as the concentration of NaCl increases so to does the speed at which the digestion took place. However, the 1 mol test showed to be an anomaly with all tests conducted showing that there was no starch digested. The errors present in this test meant that the results were invalid but showed to an extent how NaCl affects amylase in the digestion of starch.

References

Aghajari, N., Feller, G., Gerday, C. and Haser, R. (2018). Structural basis of α-amylase activation by chloride.
Bbc.co.uk. (2018). BBC - GCSE Bitesize: What are enzymes?. [online] Available at: http://www.bbc.co.uk/schools/gcsebitesize/science/add_aqa_pre_2011/enzymes/enzymes1.shtml [Accessed 7 Mar. 2018].
Biology.arizona.edu. (2018). Energy, Enzymes, and Catalysis Problem Set. [online] Available at: http://www.biology.arizona.edu/biochemistry/problem_sets/energy_enzymes_catalysis/01t.html [Accessed 7 Mar. 2018].
Cooper, G. (2018). The Central Role of Enzymes as Biological Catalysts. [online] Ncbi.nlm.nih.gov. Available at: https://www.ncbi.nlm.nih.gov/books/NBK9921/ [Accessed 7 Mar. …show more content…

(2018). Enzyme regulation. [online] Available at: https://www.khanacademy.org/science/biology/energy-and-enzymes/enzyme-regulation/a/enzyme-regulation [Accessed 25 Feb. 2018].
Levitzki, A. and Steer, M. (2018). The Allosteric Activation of Mammalian alpha-Amylase by Chloride.
Lopina, O. (2018). Cite a Website - Cite This For Me. [online] Cdn.intechopen.com. Available at: https://cdn.intechopen.com/pdfs-wm/54390.pdf [Accessed 25 Feb. 2018].
Rsc.org. (2018). Chemistry for Biologists: Enzymes. [online] Available at: http://www.rsc.org/Education/Teachers/Resources/cfb/enzymes.htm [Accessed 25 Feb. 2018].
Wang, N. (2018). Starch Hydrolysis by Amylase. [online] Eng.umd.edu. Available at: https://eng.umd.edu/~nsw/ench485/lab5.htm [Accessed 7 Mar. 2018].
Worthington-biochem.com. (2018). Effects of pH (Introduction to Enzymes). [online] Available at: http://www.worthington-biochem.com/introbiochem/effectsph.html [Accessed 7 Mar. 2018].
Secondary Data Graph
Feller, G., Bussy, O., Houssier, C. and Gerday, C. (2018). Structural and Functional Aspects of Chloride Binding to Alteromonas haloplanctis α-Amylase. [online] Jbc.org. Available at: http://www.jbc.org/content/271/39/23836/F1.expansion.html [Accessed 8 Mar.

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