Nanobiotechnology Synthesis

“SYNTHESIS AND CHARACTERIZATION OF NICKEL OXIDE BY USING UV/VIS SPECTROMETRY, SCANNING ELECTRON MICRSCOPY, FOURIER TRANSFORM INFRARED SPECTROSCOPY AND ITS ANTIMICROBIAL ACTIVITY AGAINST
BACILLUS SPP., PSEUDOMONAS SPP. ESCHERICHIA COLI”

J.C. Pradeep Kumar, Dr. F. V. Dandawate

Assistant Professor, Department of Chemistry, Dr. D. Y. Patil Arts, Commerce & Science College,
Pimpri, Pune – 411018. (Maharashtra, India)

ABSTRACT

Nanobiotechnology combines biological principles with physical and chemical procedures to generate nano-sized particles with specific functions. A green synthesis of nickel oxide and nickel synthesized by boiling method using an extract of Molinga Olefera. UV/Vis-Spectra showed the maximum absorbance of 280 nm in the

In scientific terms “nano” means 10-9 meter where one nanometer is equivalent to one thousandth of micrometer, one millionth of millimeter and one billionth of a meter. In Greek, ‘nano’ derives from the Nanos, which means dwarf [1]. It is further classified according to size: in terms of diameter, fine particles cover a range between 100 and 250 nanometers (nm), while ultrafine particles, on the other hand, are sized between 1 and 100 nm. Nanoparticles may or may not exhibit size-related properties that differ significantly from those observed in fine particles or bulk materials. Although the size of most molecules would fit into the above outline, individual molecules are usually not referred to as Nanoparticles. Particles with a size between 1 and 100 nm are normally regarded as

In this work, the boiling method was employed to prepare Nickel Oxide Nanoparticles. The materials obtained were thermally treated at various temperatures. Some chemicals can interfere directly with the proliferation of microorganisms at concentrations that can be tolerated by the host. The antimicrobial activity of Nickel oxide Nanoparticles is well known. Hence we make use of this property to inhibit growth of E.coli, Bacillus spp., Pseudomonas spp. using disc diffusion method. These two bacterial strains were selected as they are highly contagious; thence we can evaluate the potential antimicrobial activity of nickel oxide