When the end point was reached, the pH was somewhat constant at approximately 11. The procedure was repeated for a 2nd time using a fresh sample of phosphoric acid. Now the 250 mL flask was taken and approximately 50 mL of the soft drink (sprite/mountain dew) was placed into it. The flask was shaken several times to release the carbon dioxide in the drink. The flask was then vented.
(vi) Transfer the concentrate and any precipitate to a 250 ml conical flask using 5 ml concentrated HNO3. (vii) Add 10 ml concentrated H2SO4 and a few boiling chips or glass beads. Evaporate on a hot plate in a hood until dense white fumes of SO3 just appear. (viii) If solution does not clear, add another 10 ml concentrated HNO3 and repeat the evaporation to obtain fumes of SO3. Remove all HNO3 before continuing treatment.
Standardization of NaOH solution The prepared solution in part A was used to determine the acidity of the two different brands of soft drinks. But before it, the NaOH solution was standardized first. A 0.15 g of potassium acid phthalate was dissolved in 0.05 L of water in an Erlenmeyer flask. Afterwards, 3 drops of phenolphthalein was added. A 50 mL buret was obtained and was washed with NaOH solution.
The infrared absorption spectra of pure polymer and physical mixture of polymer and drug were run and between 4000 cm-1-500 cm-1 . Result and Discussion 91 Ketorolac tromethamine mouth dissolving tablet Melting point-166.6 between 165 and1 70 with decomposition and contain protein binding 99.2% For the determination of partition coefficient of human insulin, n-octanol was used as oil phase and phosphate buffer pH 7.4 was used as aqueous phase. Phosphate buffer saline pH 7.4 and n-octanol 0.64 The absorption maxima (λmax) of ketorolac tromethamine (5 µg/ml) in this solution was found to be 372 nm which is concordant with the Indian Pharmacopoeia
Vernier Caliper 5.4.4 (D) Friability: This test is performed to evaluate the ability of tablets to withstand abrasion in packing, handling and transporting. Initial weight of 20 tablets is taken and these are placed in the friabilator, rotating at 25 RPM for 4min. The difference in the weight is noted and expressed as percentage. It should be preferably between 0.5 to 1.0% % Friability= (W1-W2×100)/W1 Where, W1= weight o f tablets before test W2 = weight of tablets after test Fig: 4. Roche friabilator 5.4.5 (E) Weight variation of Tablets: It is desirable that all the tablets of a particular batch should be uniform in weight.
The capecitabine solution was added to GNPs dispersal and stirred at 1000 rpm for 30 min. The mixture of capecitabine and GNPs dispersal was incubated for 24 h at room temperature for complete loading of drug on NPs and the centrifuged at 20,000 rpm for 30 mins. The obtained pellet after centrifugation was separated from the supernatant solution and redispersed in deionized water prior to further characterization. The capecitabine concentration in redispersed pellet was determined spectrophotometrically at the λmax value of 240 nm, the percentage loading of capecitabine on GNPs was estimated by following
Then, the leaves were grinded to powder by using domestic blender and the coarse powder is stored in airtight container. Preparation of crude extracts. The coarse powder was extracted with distilled water and methanol. For aqueous extraction process, the coarse powder was extracted with distilled water at a ratio of water to leaves of 10:1 (ml:g) for 30 min at 85 °C. The extraction process was repeated one more time.
Secondly, the obtained blend was uniformly spread as a homogeneous layer on the surface of the mortar and left standing for five minutes to allow the liquid medication to be absorbed inside powder particles. Thirdly, the powder was scraped from mortar surface by means of a spatula and blended with a calculated quantity of superdisintegrant 5% (w/w) for 10 minutes. Sodium starch glycolate (SSG) was used in the all liquisolid formulations, then the final mixture was lubricated with 1% magnesium stearate for two minutes. Lastly, the prepared formulations were compressed manually into cylindrical tablets by using a single punch tablet press machine of die sizes measuring 6 and 8 mm. The optimized formulation with optimal R value, drug concentration and loading factor was determined according to the flow properties and in-vitro dissolution studies.
This diluted solution will be used in the assay as duplicate samples. Then, 1.0mL of standard glycine solution containing (7.5mg/mL) was diluted to 100mL with water using a volumetric flask. This solution contains 1.0µmole/mL of glycine. 8 tubes were set up according to the following protocol and 2.0mL of ninhydrin reagent was then added to each of the 8 tubes and were placed in a boiling water bath for 20 minutes. After 20 minutes, the tubes from the bath was carefully removed, cooled in a beaker of cold water, then 8.0mL of 50% ethanol was added and mixed well.
Preparation of the Plant Extract The leaves were washed thoroughly with distilled water. Then, it was oven-dried and pulverized into fine powder. 100g of the powdered leaves were weighed and macerated in 600mL ethanol in a stoppered flask for 24h. The solution was filtered and concentrated by a rotary evaporator. 2.3.