Neomycin Sulphate Research Paper

Neomycin sulphate was a gift of INTAS Pharmaceuticals Ltd. (Ahmedabad, India). Silver nitrate, Sodium borohydride, Polyvinyl Pyrrolidone K-30 (PVP K-30), Chitosan, Surgical Gauze and Silver sulfadiazine ointment were purchased from Qualigens fine, Merck, S. D. Fine Chemicals, Balaji Chemicals, Medicare Hygiene and Super formulation Pvt. Ltd, Ujjain respectively. All other chemicals and solvents were of Analytical Reagent grade. Milli Q water was used throughout the experimental work.
2.2 Method for determination of Neomycin sulphate drug
Accurately weighed 100 mg neomycin sulphate was dissolved in 100 ml distilled water (1000μg/ml). Stock solution was prepared by taking 10 ml of this solution and diluting it upto 100 ml with distilled water (100μg/ml). From the stock solution, 1 ml solution was withdrawn and transferred to 10 ml volumetric flask to which 1 ml of 1% Ninhydrin solution was added and heated on water bath until light purple color was obtained. 2 ml, 3 ml, 4 ml, and 5 ml solution was withdrawn and same procedure was repeated as above. Solutions were diluted to get concentration of 10 μg/ml, 20 μg/ml, 30 μg/ml, 40 μg/ml and 50 μg/ml neomycin sulphate respectively and scanned under the UV-VIS spectra were recorded between 400-800 nm. Absorbance was measured at 566nm. Similar procedure was used for the measurement of the drug content. [19]

[20]. Synthesized silver nanoparticles were characterized by UV- Vis absorption spectroscopy, Dynamic Light Scattering (DLS) and Zeta potential and Atomic force microscopy