Neomycin sulphate was a gift of INTAS Pharmaceuticals Ltd. (Ahmedabad, India). Silver nitrate, Sodium borohydride, Polyvinyl Pyrrolidone K-30 (PVP K-30), Chitosan, Surgical Gauze and Silver sulfadiazine ointment were purchased from Qualigens fine, Merck, S. D. Fine Chemicals, Balaji Chemicals, Medicare Hygiene and Super formulation Pvt. Ltd, Ujjain respectively. All other chemicals and solvents were of Analytical Reagent grade. Milli Q water was used throughout the experimental work.
2.2 Method for determination of Neomycin sulphate drug
Accurately weighed 100 mg neomycin sulphate was dissolved in 100 ml distilled water (1000μg/ml). Stock solution was prepared by taking 10 ml of this solution and diluting it upto 100 ml with distilled water (100μg/ml). From the stock solution, 1 ml solution was withdrawn and transferred to 10 ml volumetric flask to which 1 ml of 1% Ninhydrin solution was added and heated on water bath until light purple color was obtained. 2 ml, 3 ml, 4 ml, and 5 ml…show more content… Gram-negative bacteria, Escherichia coli strain B (E. coli) and gram positive bacteria Staphylococcus aureus were taken for antimicrobial assay. each plate was fixed at equal distance. First disk was loaded with 10 μl of distilled water which served as control. Remaining disc i.e. 2nd to 7th were impregnated with 10 μl of silver nanoparticle, 10 μl of neomycin sulphate solution, 10 μl of chitosan solution, 10μl of chitosan-silver nanoparticle solution, 10μl of silver nanoparticle-neomycin sulphate solution and 10μl of silver nanoparticle-chitosan-neomycin sulphate solution respectively. All these plates were kept at 37⁰C for incubation overnight. After 24 hrs of incubation, zone of inhibition were measured and compared.