Diltiazem Hydrochloride And Nifedipine

Chapter3. AIMS AND OBJCETIVE OF THE STUDY
The Project is aimed at development of mucoadhesive drug delivery system of antihypertensive drugs diltiazem hydrochloride and nifedipine respectively. These drugs are selected on the fact that both drugs are having short half life, both undergoes hepatic metabolism at the same time both gets absorbed from gastrointestinal tract rapidly, though diltiazem hydrochloride is hydrophilic and nifedipine is hydrophilic.
Marketed formulations are available in the form of sustained release tablets capsules. So alternative drug delivery system can serve the purpose of delivering the drug at a particular site and which can bring about slow release and improved bioavailability may be achieved is needed .
Plan

The solution was shaken intermittently to assist the attainment of equilibrium with the undissolved drug particles. Then measured quantity of the filtered drug solution was withdrawn after 24 hrs and successively diluted with respective solvents and the concentration was measured spectrophotometrically. Average of triplicate readings was taken

Analytical method A suitable method of estimation was developed for drugs namely diltiazem hydrochloride and nifedipine in methanol and phosphate buffer pH 6.8.

Standard graph of Diltiazem hydrochloride in methanol and phosphate buffer pH7.4 Procedure
Calibration curve for estimation of Diltiazem hydrochloride Spectrophotometric method is based on observed λ max of 238 nm in UV-region using phosphate buffer pH 7.4 and methanol was used for estimation of Diltiazem hydrochloride in the present study.

Preparation of buffer
 Preparation of phosphate buffer pH 7.4: Potassium dihydrogen phosphate solution of 0.2 M was prepared and 250 ml of this solution was mixed with 195.5 ml of 0.2 M NaOH and volume was made up to 1000ml with distilled water. The pH of the buffer was adjusted to 7.4.
 Scanning of drug (Determination of