As many as 1 mL hydrogel preparations added in 1 mL of growth medium with stratified so that dilution dilution series made were 50%, 25%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78%, 0.39%, 0.02%, and 0.01% by volume end of the tube was 1 mL. Then as much as 1 mL of bacterial culture equal Mc. Farland 0.5 added into the test tubes so that the final volume of the tubes were 2 mL. All test tubes were incubated at 37 °C for 18 h. Turbidity test observed in the media and determined MIC value preparations. Tube with negative results or does not indicate the presence of growth, then conducted subculture with solid growth media each bacteria as test assertion MIC value chloramphenicol preparations hydrogel.
WHO classifies lindane as “Moderately Hazardous” pesticide. The USEPA (2005) has determinated that lindane does not contaminate drinking water in excess of the Agency’s level of concern8. In water, sediment and fish samples collected from Maleksaban lake (S-6), residual concentration of ∑HCH ranged from BDL-1122.42μg/l, BDL-1275.26 ng/g and BDL-689.74 ng/g respectively. In the case of Nikol Lake (S-7), residue of ∑HCH concentration ranged from BDL-119.65μg/l, BDL-224.35 ng/g and BDL-23.01 ng/g respectively. If we compare the residues of organochlorine pesticides (OCPs) between different sampling sites, the site-6 from Maleksabanlake has maximum concentration of these persistent compound 1263.96 ng/g in sediment whereas the level is 1106.01μg/l in water sample
 Appropriate concentrations of SBP hydrolysate were mixed 1: 4 (v/v) with 200 M DPPH solution in anhydrous methanol for 30 min in the dark. The absorbance of the samples was recorded spectrophotometrically on a microplate reader (Multiskan Go, Thermo Fisher Scientific) at 517 nm. A control group containing DPPH solution without sample was also prepared. Ascorbic acid was used as a positive control. ABTS radical scavenging
Adequate storage and inventory control is a challenge many drugs expire before they can be used, or they are used irrationally. Eventually, the products reach the service delivery point, which might be a health facility, laboratory, or community health worker. Finally, at the point of delivery to the customer, there is product use. Availability of health commodities alone does not ensure quality of care. Drugs must also be rationally prescribed and dispensed; and clients, especially providers, must be aware of the treatment guidelines for the products.
Further, 1ml, 2ml, 3ml, 4ml & 5ml was taken from above diluted solution and dilute it to 10 ml with phosphate buffer pH 7.4. The absorbance of resulting solution was measured at 480 nm. The absorbance values were graphically represented in Figure.
Higuchi model94,95 [Q = KH t½] 4. Korsmeyers-Peppas model:96,97 F = (Mt/M) = Km tn 6.4.7. Drug content: The drug content from pellet formulations was investigated; in which the quantity of pellets equivalent to 6.25 mg of dose of zolpidem tartarate was weighed and dissolved in 0.01 N HCl, sonicated for 15 minutes to dissolve it completely and then the solution of 14 ppm was prepared which was considered as a working level for the complete analysis. The absorbance was determined at the 294.5nm by UV spectrophotometer. Then the drug content was determined by comparing the absorbance of this solution with standard solution having same concentration.
Diazotized Sulphanilic Acid 1. Dissolve 1.1 g of anhydrous sodium carbonate in 50 mL of water in a 100 mL conical flask. 2. Add 4 g of sulphanilic acid to the solution and heat it until it dissolves. A small amount of suspended material may render the solution cloudy.
The volume was made with 6.8 pH Phosphate buffer to get a concentration of 1000µg/ml (SS-I). UV Absorption Maxima (λ max) of Itraconazole sample in pH 6.8 Phosphate buffer • Stock II: 10ml of above solution was then further diluted to 100ml with 6.8 pH Phosphate buffer to get a stock solution of 100µg/ml. UV scanning was done for 100 µg/ml drug solution from 200-400 nm using pH 6.8 Phosphate buffer as a blank in Shimadzu, UV 2450 spectrophotometer. The wavelength maximum was found to be at 262
The result is expressed in mg of quercetin/g dry weight compared to the standard curve of quercetin, which is made in the same condition of the sample (Ghasemzadeh et al. 2010). 22.214.171.124. Antioxidant Activity Analysis From the extraction solution was taken 0.1 ml and react with 3.9 ml solution DPPH (2,2-diphenyl-1-picrylhydrazyl) 0.024 mg / mL for 30 minutes at room temperature and covered with aluminum foil. Measure sample absorbance in t = 0 and t = 30 with the spectrophotometer at wavelengths 515 nm.
In summary , alternative medicine satisfies patients with no chemical treatment plus it is so beneficial to human being .Contrary to that opinion , alternative medicine has no scientific basis and is has been misused by some advertisements to gain profits. Alternative medicine has been criticized for taking too long to heal or to get a result but in fact the overall help to the body that occurs last much longer with benefits to the body system