Detection and quantitation limits Sensitivity of the developed method was evaluated by calculating the detection and quantitation limits. These limits were calculated using the formula; LOD=3.3σ/S and LOQ=10σ/S for limits of detection and quantitation, respectively, where σ is the standard deviation of intercept and S is the slope of calibration curve. The obtained LOD and LOQ values were 0.022 and 0.068 μg mL-1, respectively (Table 2). These values indicates that the high sensitivity of the developed method as it can quantify a concentration of the studied drug down to the nano-gram level. 3.5.3.
• Omit the process approaches like nanonisation, micronization techniques. 2.5.4 Disadvantages  • Formulation of high dose lipophilic drugs the liquisolid tablet is one of the limitations of this technique. • In order to achieve acceptable flowability and compactability for liquisolid powder formulation, high levels of carrier material and coating materials should be added. This will increase the weight of tablets to above one gram which makes them difficult to swallow. Consequently, it is impossible with conventional tablet methods to convert high dose to liquisolid tablets with a tablet weight of less than 50mg.
The solution homogeneity expelled, by centrifugation for 10 min. The sample was centrifuged and separated into two layers, and took the top of the sample is injected for HPLC (11,12). Measured concentration in total lipid: The total fats balanced concentration of the pesticide getting by dividing the measured pesticide residue concentration in the overalll tissue sample by the decimal fraction of the sample that consisted of ether-extractable lipid. The total lipid content of each specimen was estimated from its total cholesterol & triglycerides levels by using a summation method. Analytical results for organochlorine pesticides were reported on a lipid-adjusted basis (nanograms per gram or parts per billion) (14).
3.1 Particles size analysis The microgels particles characterized by DLS were reported previously . The hydrodynamic diameter was determined by Stokes-Einstein relations. Fig. 1 illustrates the temperature dependent particle size and size distribution of copolymer microgels with different AMPS content in water at 25oC and 50oC. The data shows that the hydrodynamic diameters of microgels increases from 343.4 nm to 538 nm at 25oC and 132.5 nm to 414.8 nm at 50oC with increasing AMPS concentration in the feed recipe but its diameters reduces with increasing temperature.
Briefly, the cartridges were preconditioned by flushing with 2 mL of methanol and 1 mL of HPLC water. Separately, 50 µL of plasma sample plus 100 µL of an 85% phosphoric acid:water mixture (1:10) and 10 µL of internal standard solution (diclofenac at 100 µg/mL) were vortex mixeding. Then, samples were loaded into the cartridge and allowed to stand for 5 min, washed with 0.6 mL of a water:methanol mixture (95:5. v/v) and then dried under vacuum. The (S)-ketoprofen was eluted with 1 mL of an acetonitrile:methanol mixture (50:50, v/v) at a flow rate of 1 mL/min. The eluate was evaporated to dryness in a water bath at 37.0 ± 0.5 ᵒC under a gentle stream of nitrogen.
At different time in the range of 5min to 300 min, the sample will be collected and its total phenolic content (mg GAE/ g dry weight) was determined. Effect of stirrer speed on extraction of phenolic compounds The solvent with the optimized concentration will be considered to study the effect of mixing on the sample at 300C. The stirring speed was maintained in the range from 200 rpm to 800 rpm. At different time in the range of 5min to 180 min, the sample was collected and its total phenolic content (mg GAE/ g dry weight) was determined. Effect of pH on extraction of phenolic
2. Materials and methods 2.1. Materials Analytical grade Fe(NO3)3.9H2O, Na2SO4, NaHCO3, and H2SO4 were obtained from Merck. Reagents such as pluronic acid P123, hydrochloric acid and tetraethylorthosilicate (TEOS) were collected from the Sigma Aldrich and Rhodamine B was purchased from Loba Chemie chemical company. These reagents and dyes were directly used without further purification.
The optimized ratios of the polymers were calculated by using 22 factorial design. (Table 1). Ethyl cellulose (2gm) was dissolved in 20 ml of methylene chloride2. The polymer phase of ethyl cellulose was then added to 250 ml of 0.25% w/v methylcellulose aqueous solution (over night dispersion). Agitation speed was maintained at 350 rpm which helps in complete removal of methylene chloride.
Effect of the temperature on conversion (8 g/L catalyst loaded membrane, M=1) Conversion was increased by temperature in both of the BR and the CMR. However, conversion of CMR was higher than conversion of BR. In CMR, water, which occurred from the result of the reaction, was removed from the reaction medium. Therefore, reaction equilibrium shifted toward the products. Thermal mobility of the polymer chains increased with increasing temperature.
Nanoparticle images and selected area electron diffraction (SAED) were recorded by using the transmission electron microscopy (TEM) (TECHNAI-20-G2) by drop-casting the well-sonicated solution of a few milligrams of nanoparticles dispersed in 5 ml of ethanol on carbon-coated TEM grids. The hydrodynamic size and surface charge of nanoparticle dispersions were measured using a Zetasizer (Malvern ZS-90) utilizing dynamic light scattering (DLS). DLS is the most intense and frequently used technique for hydrodynamic size distribution measurement and they were performed after thorough sonication of the nanoparticles dispersed in different dispersants. Approximately 2 mg of nanoparticles were dispersed in 10 ml of the dispersant, i.e. water, ethanol, and toluene, for a typical run.