5.1 Materials and Methods The following materials were used from the sources, without further purification. Diclofenac was procured as a gift sample from M/s Yarrow Chem Products, Wadala (E) and Mumbai. India. Cholesterol, Oleic acid, Triethanolamine (TEA), Acetone and Ethanol were obtained from Central Drug House Pvt. Ltd. New Delhi, India. All the other chemical were of the analytical grade. Double distilled water was used throughout the study. 5.2 Method For Preparation of Nanostructured Lipid Carriers (NLCs) 5.2.1 Plain nlcs (Without Drug) Melt emulsification and low temperature solidification method The formulations of different ingredients are shown in Table 1. Diclofenac and phospholipon 90G were dissolved in methanol and mixed …show more content…
5.3 OPTIMIZATION OF DRUG LOADED NLCs Preparation of Diclofenac NLCs involves various process variables out of which following were selected for the optimization of the formulation: 1. Effect of varying polymer concentration (Stearic acid). 2. Effect of varying drug concentration (Diclofenac). 3. Effect of varying stirring speed. 5.3.1 Effect of varying polymer concentration NLCs were prepared by method reported in section 5.2.1 by using varying stearic acid : Oleic acid concentration viz. 1:1, 2:1, 3:1 and 4:1 mg while other variables were kept constant. The effect of varying stearic acid concentration on the particle size and drug entrapment efficiency are reported in Table 5.1 and shown in Fig. 5.1 …show more content…
Analysis was performed at 25 ± 2oC. The NLC dispersion was diluted with double distilled water. Few drops of the dispersion were placed on the cover slip and allowed to dry in rotator drier to form a hard film. After formation, the images were captured (Fig. 5.4). Fig. 5.4: SEM analysis of of NLC. 5.4.2 Determination of particle size, polydispersity index These measurements were performed in triplicates. The particle size and polydispersity index (PI) of nanostructured lipids were determined by Zetasizer Nano ZS (Malvern Instrument), Malvern, UK) at a temperature of 25±2oC at 90oC to the incident beam applying the principle of photon correlation spectroscopy. Dispersion were diluted with double distilled water to ensure that light scattering intensity, was within the instrument intensity range (Table
C4564 Description: IC50: 3-AP is a ribonucleotide reductase inhibitor and iron chelator with antitumor activity. Ribonucleotide reductase, the rate-limiting enzyme for de novo DNA synthesis, is an excellent target for chemotherapy. Its increased activity in cancer cells is associated with malignant transformation and proliferation.
The design relied on two Schmitt triggers to generate the two different tones while using the transistors to act as a switch. This causes it to trigger continuously between two unstable states, allowing automatic switching between two frequencies producing two different tones. The RC values between the two Schmitt triggers will differ. Capacitors charge and discharge faster when it’s resistance is smaller.
III SYNTHESIS AND SIMULATIONS RESULTS The simulation and synthesis work is finally done by the xilinix and modelsim respectively. Figure 5:synthesis results of Fault FFT. The figures intimate the fault injected FFT,which is checked by the manual error injected via all diferent possibilities by using RTL scripting. Eventhough the soft error is added in the FFT the error detector code 100% detect the errors and corrector correct the errors.
Results The lab experiment was done in two parts, one with the NAND, NOR, XOR and Hex Inverters and the other with a 7483 full adder gate, both will verify the truth table when two input bits and a carry are added together. The circuits were built by examining the 1 bits through a K-Map to create a Boolean expression for the sum and carry. The Boolean expression for the sum was A⊕B⊕C and the carry as AB+BC_in+AC_in. From these two expressions, we notice that we must use two exclusive-ORs gates in the sum inputs for A, B, and C. For the sum, we have to use NOR and NAND (the only available gates from the lab manual).
For most sequences at position 4 and 5 we observe only the nucleotides G and T, respectively. There may be rare cases where other nucleotides may also be found. To consider such observations, we need to do a process called additive smoothing or Laplace smoothing to smooth the categorical data. [9] In this case, we add 4 sequences: AAAAAAAAA, CCCCCCCCC, GGGGGGGG, TTTTTTTTT.
I need to find the area of rectangle ABCD. I know that ABCD is a rectangle with diagonals intersecting at point E. Segment DE equals 4x-5, segment BC equals 2x+6, and segment AC equals 6x. I predict that To find the area of rectangle ABCD I need to find out the base and height of the rectangle. The first step is to find what x equals. Since I know the intersecting line segments AC and DB are congruent that means when I times the equation 4x-5 for segment DE by two it will equal the equation 6x for segment AC.
Experiment 7 In this experiment we configured several DC circuits consisting of an emf and a network of resistors. The circuits were composed of a power supply, two DMMs, a circuit board, an SPST switch, and an assortment of known resistors along with one unknown resistor. We measured the current and voltage of the entire circuit as well as the potential drops across each resistor to determine the parameters of the circuit including the resistance, voltage, and current for each component.
Using the data provided in each one of these tests it can be assumed that one has done the steps to be able to determine the magnitude and orientation of the charges of the tape in each test, thus, allowing them to apply the same principle to any object they so desired. Their results would line up with the following; that if the two pieces of tape are torn from the same 40 centimeter strip then the tops of both pieces of tape would be positive and the bottoms of both pieces of tape would be negative and that if they would double the tape the attraction or repulsion in general would lower due to the increased density. Their data would also show that two pieces of tape ripped from each other would result in one piece being entirely positive and the other being entirely negative, they would also be able to state that the orientation of how the tape is paired up doesn’t matter.
1. The test subjects will prepare for sleep by acquiring everything needed for the subjects’ sleep preferences. 2. The test subjects will all set alarms on their smartphones for approximately 6, 8, and 10 hours after the subjects’ enter the resting period (Subjects may wake during the resting period for the bathroom, but they must not stay awake for more than ten minutes at a time to prevent as much deviation as possible.). 3.
1. There are two ways of maximizing points in this experiment. The first one is that I should connect myself to a vertex that is in the biggest component and purchases immunization. Since the probability of being infected is based off of expected value, I would have less than 1% chance of getting infected. The second way is that I try to make myself stay in the second-largest connected component.
1. What area/aspect of this setting is the most challenging? 2. In the setting, you work in, is there a certain population of patients you see more? How does this affect you?
1. Identify the range of senses involved in communication • Sight (visual communication), Touch (tactile communication), Taste, Hearing (auditory communication), Smell (olfactory communication) 2. Identify the limited range of wavelengths and named parts of the electromagnetic spectrum detected by humans and compare this range with those of THREE other named vertebrates and TWO named invertebrates. Figure 1: the electromagnetic spectrum source: www.ces.fau.edu Vertebrates Human Japanese Dace Fish Rattlesnake Zebra Finch Part of electromagnetic spectrum detected ROYGBV (visible light) detected by light sensitive cells in the eye called rods and cones.
The following lab period the solid was weighed (0.0483 g) and percent yield was calculated (65.5%) with the limiting reagent being tetraphenylcyclopentadienone. The melting point was determined. The first melting point was 204-204.9 °C and the second melting point was 215.6-215.9°C. Finally, an infrared spectroscopy was obtained for the
Tn 4351 was originally isolated from bacteroides fragilis [30] . The transposon was successfully introduced into Cytophaga succinicans, Flavobacterium meningosepticum, Flexibacter canadiansis, Flexibacter strain SFI and Sporocytophaga myxococcoides by conjugation [25]. Tn 4351carries two antibiotic resistance gene. One of the codes for resistance to erythromycin and clindamycin which is expressed in bactroides but not in E.Coli. The other gene codes for resistance in tetracycline and is expressed in aerobically grpwn E. coli, but not in anaerobically grpwn E. coli or in bacteroides.
The developing solution was poured into a tank and was tightly covered with a glass lid, and the tank was allowed to be saturated to ensure that the solution was equilibrated in the gas phase. Silica plate for TLC analysis: A horizontal line was drawn with a pencil on the plate and it was about 1 cm above the bottom of the plate. The horizontal line was drawn faintly so as to avoid damaging the silica gel on the plate. On the horizontal line, two marks were made and one was named A and the other B. These marks were made towards the centre of the plate at a distance apart because when spots are made at the edge of a plate, the result would be an improper travel of the samples as the solvent advances on the plate.