Tissue Culture Case Study

Pour this mixture, while stirring, into a beaker containing 25 mL of ice water to which 5 mL of concentrated hydrochloric acid have been added. The diazonium salt of sulfanilic acid should soon separate as a finely divided white precipitate. Keep this suspension cooled in an ice bath until it is to be used. 2. Methyl Orange 1.

Heat the Agarose gel in a 65 °C water-bath to melt the agarose. After it melted, maintain its temperature at 55 °C until it is ready for use. 2. Transfer the spheroids from the 96-well plate to a 15 mL centrifugal tube using a 1000 μL pipette 3. centrifuge the tube for 5 min at 1000 rpm to form a pellet.

After the finalization of alkali formulation to be used for extraction of cornhusk fibres, the fibres were treated with Pulpzyme HC to increase their fineness. Pulpzyme HC is a xylanase enzyme (EC.3.2.1.8) produced by submerged fermentation of a genetically modified Bacillus microorganism, and was obtained from Novozymes. The enzyme has an activity of 1000 AXU per gram. (http://fzfz.nbdl.gov.cn:81/files/20130815/1376527490502_36.pdf) Effect of concentration of Pulpzyme HC and Treatment Time The extracted fibres were treated with three different concentrations of enzyme i.e. 1%, 3%, 5% (owf).The time taken for treatment was 30 minutes, 60 minutes and 90 minutes.

22.5 g of plate count agar powder was dissolved in a litre of sterile distilled water on the hot plate 2. pH of the solution was adjusted to 7.0 ± 0.2 by adding NaOH or HCl and was immediately transferred into the Schott bottle to be autoclaved at 121 ° C for 15 minutes 3. Prepared medium was stored in 4° C chiller Lauryl Sulphate Broth

Then, it is air dried and followed by fixing it with flame from Bunsen burner. After fixing the smear, it must be stained using Gram staining solution, firstly crystal violet solution was flood onto it, and allowed for 1 minute, then wash off with tap water. Then, flood the slide with iodine solution for 1 minute and wash it off with tap water again. The formation of a dye-iodine complex will occur in the cytoplasm. Then, it was flooded with ethanol and washed immediately.

2. Materials and methods 2.1.Yeast cells and culture growth conditions Yeast cells S. cerevisiae VIN 13 strain (commercial dry yeast) used for laboratory experiments were provided by Anchor Yeast (South Africa). Yeast cells were grown in a defined medium containing (per liter of deionized water): 100 g D-glucose, 1 g K2HPO4, 1 g K2H2PO4, 0.2 g ZnSO4, 0.2 g MgSO4, 2 g yeast extract and 2 g NH4SO4. All the media components were purchased from the Sigma Chemical Company (St Louis, MO, USA). 2.2.Yeast cell preconditioning and inoculum preparation 1 g dry weight of yeast was resuspended in 100 mL of deionized water in an Erlenmeyer flask of 250 mL volume, at 30–35°C, for 30 min with NaCl 6% w/v. Inoculum for experimental fermentations was prepared

Pour 50 ml of 1% agarose solution kept at 50ºC onto the casting plate. Immediately place the supplied comb about 1 cm from one end of the plate ensuring that teeth of the comb do not touch the glass plate. Wait till a firm layer of gel is

H2SO4 • Catalyst: potassium sulphate (K2SO4), copper (II) sulphate pentahydrate (CuSO4.5H2O) • NaOH (40%) • 0.1 N HCl solution • Boric acid (4%) • Indicator: methyl red (200 mg make up to 100 ml with %95 ethanol ) 1 gram Feta Cheese was used as a sample in this experiment. First of all, the experiment was started with the digestion phase which was performed by mixing 10g potassium sulphate and 0.5 g CuSO4 solution that acts as catalyst, and added 20ml conc. H2SO4. The digestion unit temperature was set to 420±10°C and preheated for 15 minutes.

Make the solution up to the 500cm3 mark with iodine (1% concentration) • Starch Indicator Solution Weigh 0.25g of soluble starch and add it to 50cm3 of near boiling water in a 50cm3 beaker. Stir it in order to dissolve and wait for it to cool before using. Procedure Safety • Before conducting the experiment, be sure to put on safety goggles and

White solid was formed in the process of addition and the solution was then left undisturbed in an ice bath for 10minutes. Once most of the solids had settled at the base of the beaker, the solution was decanted. 10ml of ethanol was added to the remaining suspension and was transferred in a clean centrifuge and centrifuged for 2minutes at 6000rpm. After the first centrifugation, the supernatant was discarded and the residue was washed by adding 14ml of ethanol. The subsequent centrifugation was repeated twice, only this time using 8ml of diethyl ether instead of ethanol.

Sourdough Starter Ingredients 1 pkg. active dry yeast 2 1/2 cups warm water 2 cups all-purpose flour 1 tablespoon sugar Directions Dissolve the yeast in 1/2 cup of the warm water. Stir in the remaining 2 cups of water, the flour and the sugar and beat until smooth. Cover with cheesecloth and let stand at room temperature for 5-10 days or until bubbly.

Preheat the oven to 375 degrees and line an 8” x 8” square pan with foil. Set aside. 2. To make the graham cracker crust, crush the graham crackers up and mix in the melted butter and sugar. Pour and spread the graham cracker mixture into the pan and push down to make an even layer.

Before starting the heating process, measure the weight of the crucible with its cover first and then tare the balance, and after that adding about 1 gram of the sample to the crucible with its cover, and then weigh it. Moreover, it is possible liberating harmful gases during the process of heating; therefore, being careful is important. The heating process ends when this sample changes the color to brown because water of hydration is removed to the sample. Additionally, give time to the small cool down and measure its weight. Next, transfer the sample to a 50 mL beaker and mixes with distilled water, which gets by rinsing the crucible with its cover in 8mL, so the solution is generated.

5. 150 ml of the solution in beaker A was added to the separating funnel with 10ml of chloroform. The funnel was gently shaken and vented to release the pressure. This was done five times. 6.

The silver ion TLC was prepared through the following procedure: Silver nitrate was dissolved in 10 ml of distilled water. This aqueous solution of silver nitrate was absolutely mixed with 9 g of silica gel (10 ~ 40 μm particles). Then, a 10 × 5 cm TLC plate was coated with the above slurry and activated for 1 h at 90 °C before use. They were immediately transferred into a desiccator in dark for storage after cooling. 32 100 μL of afore-prepared sample solution and the mixed reference standard were diluted 100 times with ethyl acetate.