ipl-logo

Nucleotide Excision Repair Lab Report

197 Words1 Page

Nucleotide excision repair is an important DNA repair system that corrects UV induced genetic damages. One example of such damage is the formation of a covalent bond between two adjacent thymine bases on a DNA strand (thymine dimers). This causes a bulk in the DNA strand and disrupts DNA replication.
The process includes the proteins UvrA, UvrB and UvrD as well as DNA polymerase and DNA ligase. First, a protein trimer, which is composed of two UvrA molecules and one UvraB molecule, scans the double helix for any distortions. Once a thymine dimer is detected, the trimer stops. The two UvrA proteins are released leaving behind UvrB. UvrC attaches to the damaged site and makes two incision on either side of it. A helicase known as UvrD then binds

Show More
Related

  • C4564 Unit 2 Lab Report

    202 Words  | 1 Pages

    C4564 Description: IC50: 3-AP is a ribonucleotide reductase inhibitor and iron chelator with antitumor activity. Ribonucleotide reductase, the rate-limiting enzyme for de novo DNA synthesis, is an excellent target for chemotherapy. Its increased activity in cancer cells is associated with malignant transformation and proliferation.

    Read More

  • Solid Sequencing Platform, Produced By Technologies/Applied Biosystems

    944 Words  | 4 Pages

    After multiple cycles of ligation, detection and tail cleavages, the extended chain reached the end of the template. Then the whole extension chain is removed and a new starting primer switching down 1 nucleotide position binds onto the template for another cycle of reaction. Totally, five round of primer binding cycles are performed to complete the sequencing of each fragment. 3. Pitfalls and limitations of NGS Errors could be introduced in any step of the sequencing process, including library

    Read More

  • Nt1310 Unit 9 Lab Report

    1392 Words  | 6 Pages

    1. Identify the range of senses involved in communication • Sight (visual communication), Touch (tactile communication), Taste, Hearing (auditory communication), Smell (olfactory communication) 2. Identify the limited range of wavelengths and named parts of the electromagnetic spectrum detected by humans and compare this range with those of THREE other named vertebrates and TWO named invertebrates. Figure 1: the electromagnetic spectrum source: www.ces.fau.edu Vertebrates Human Japanese Dace Fish Rattlesnake Zebra Finch Part of electromagnetic spectrum detected ROYGBV (visible light) detected by light sensitive cells in the eye called rods and cones.

    Read More

  • Mitochondria Lab Report

    737 Words  | 3 Pages

    During this experiment, mitochondria were isolated from 20.2 grams of cauliflower using extraction buffer, filtration through Miracloth, and centrifusion. Twelve samples containing various volumes of mitochondrial suspension, assay buffer, DCIP, sodium azide, and citric acid cycle intermediates were prepared to be read by a spectrophotometer. The inclusion of the dye DCIP allowed for the absorbance of the reactions between the mitochondrial suspension and the TCA cycle intermediates succinate, malonate, and oxalate to be measured, as DCIP turns from blue to colorless as the activity of succinate dehydrogenase increases. Experimental Findings Increasing the number of mitochondria in the reaction did increase the reduction of DCIP relative to the amount of mitochondrial suspension present.

    Read More

  • Myrtle Rust Fungus

    670 Words  | 3 Pages

    The key enzyme in the process is DNA polymerase that forms the PCR product by linking individual nucleotide bases (adenine, guanine, cytosine and thymine). Short DNA fragments with sequence complimentary to target DNA are called primers; these specify the DNA sequence that has to be amplified. The PCR reaction mixture is made in a 96 well plate or test tube and placed in a thermal cycler. The thermal cycler is a machine used to amplify DNA by altering the temperature in repeated cycles using three precise pre programmed steps: Denaturation – By increasing the temperature in the thermal cycler the DNA is denatured creating separate single stranded DNA

    Read More

  • Dna Synthesis Lab Report

    1637 Words  | 7 Pages

    Abstract: Molecular analysis of DNA encompasses a series of separation, amplification and detection techniques that are used to determine the source of origin of an organism’s tissue sample. It correlates genes’ sequences with their functions, and allows the identification of the unknown organism. This study was done to see whether the techniques of molecular genetics like extraction and polymerase chain reaction could be used to find the animal whose tissue were sampled. GENEIOUS software was used to analyze and align the electropherograms results before GenBank and BLAST were used to identify the unknown DNA sequence by comparing it to a set of already known sequences. The results indicated that the better the fragmentation of the DNA sequences were in the PCR, the better it would be assayed by electrophoresis and the more samples could be used in the CSR; thus, the more accurate the sequences would be.

    Read More

  • Nt1310 Lab 1

    401 Words  | 2 Pages

    In transcription, DNA polymerase unwinds DNA and makes the

    Read More

  • Restriction Enzyme Lab Report

    866 Words  | 4 Pages

    Function of Restriction Enzymes: Restriction endonucleases cleave the phosphodiester bond between an adjacent phosphate and deoxyribose group in the phosphate backbone of the DNA. The active site of the endonuclease perform this cleavage by binding to the side chain of certain amino acids to the phosphate group through a chemical bond. This dissolves the preexisting bond between the deoxyribose sugar and the phosphate resulting in a breakage with in the DNA chain at a specific location. (3, 7) One characteristic feature of restriction endonucleases is that they cut at a very particular site having a specific DNA sequence. This specific sequence that allows the enzyme to attach is known as the recognition site.

    Read More

  • Dissection Lab Report

    1335 Words  | 6 Pages

    DISSECTION METHOD TO APPROACH THE HUMAN CORACOID PROCESS OF THE SCAPULA 3.1 Introduction Dissection is a traditional approach to medical laboratory education(Waters, 2008). Using human cadavers one of the most widely used model in medical and clinical research for several decade .Considerable amount of literature have been published on different dissection methods of human body .(Romanes et al.,1986;Tank et al.,2008). These currant dissection manuals showed different approach to access different part of human body.

    Read More

  • Mammalian Timeless

    215 Words  | 1 Pages

    DNA replication is a fundamental aspect of life & is essential for preservation of genome integrity. Environmental factors or drugs can cause DNA damage or lesions that lead to arrest of DNA replication forks. Replication fork arrests are among the most serious threats to the genomic integrity because they might collapse, break & are thought to be cause of many mutations & chromosomal aberrations (1-2). Eukaryotic cells are equipped with a complex repertoire of responses to DNA damage including several pathways of DNA repair & cell cycle checkpoints (3-5). In humans, defects in this checkpoint can cause genetic instability, which in turn leads to a variety of genetic disorders & cancer (6).

    Read More

  • Skin Aging Essay

    998 Words  | 4 Pages

    Intrinsic factors and extrinsic factors, both contribute to this process. As discussed earlier, UV-induced damage to the DNA causes a poor renewal of the skin. When exposed to harsh sunlight, an individual is also exposed to the UV light that comes along with it. Excessive exposure can cause premature aging in certain individuals and also is observed as severe pigmentation and sunburns as well. The antioxidants, namely vitamin E and vitamin C are majorly targeted by the UV light and thus, reduce the antioxidant capacity of the epidermis on prolonged exposure.

    Read More

  • Informative Speech On Dna

    860 Words  | 4 Pages

    DNA has a massive job of keeping you alive. In essence, a microscopic strand of genes support your entire body and life. There are many smaller jobs protein has to accomplish that combine to accomplish the main job of supporting life. To start, DNA codes for proteins and every protein provide an essential biological function. Also, cells make up tissues, organs, and body systems.

    Read More

  • Eukaryotes Vs Prokaryotes Essay

    573 Words  | 3 Pages

    Eukaryotes vs. Prokaryotes in DNA Replication DNA was first discovered in the 1860s by Friedrich Miescher and name nuclein, due to the recovery of these chemicals from the nucleus of a cell (Biology, 2015). DNA, deoxyribonucleic acid, is a unique, hereditary, chemical present in most living organisms. DNA presents in two distinct areas in the body; the majority existing in the nucleus as nuclear DNA, with a minor amount in the mitochondria, mtDNA. DNA consists of four main chemical bases, i.e. adenine, cytosine, guanine, and thymine, which in turn, form specific base pairs. Moreover, these base pairs, nitrogenous in nature, attach to a sugar (deoxyribose) and phosphate molecule, labelled as a nucleotide, and arranged in a spiral called a double helix.

    Read More

  • Therapeutic Cloning Speech

    717 Words  | 3 Pages

    Introduction Today I’m going to talk about Therapeutic cloning. What is Therapeutic Cloning? Therapeutic Cloning is a technique in medical science that you can help people to get rid of some disease that they have. Scientist has already succeeded in the experiment on healing brain disease and Parkinson disease on mice.

    Read More

  • Induced Gene Therapy

    1564 Words  | 7 Pages

    Introduction Telomeres are the ends of chromosomes that consist of tandem repeats of DNA sequences, with the length varying in various species. (Wong et al., 2008). The specific role of telomeres is to protect and guard the chromosomal ends from being damaged, and so if they become too short they lose their protective nature and leave the chromosomal ends exposed to damage (Wong et al. 2008). Types of damage that can be done to telomeres include degradation, recombination, as well as activities that repair DNA and may shorten the telomeres (Samper et al., 2001). The enzyme telomerase lengthens chromosome ends and restores length of the telomeres (Marion et al., 2009).

    Read More

More about Nucleotide Excision Repair Lab Report

Open Document