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O Toluidine Vs Hexane

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Based on the data, the eluent system that represented the best separation of the three compounds, anisole, benzoic acid, and o-toluidine, was the 4:1 hexanes to ethyl acetate ratio because it showed the greatest distance between the compounds. The highest Rf values indicated that the least polar compounds, which traveled the furthest distance, were least attracted to the polar plate due to the smaller amounts of hydrogen bonding that occurred between the compound and the plate. Anisole had the highest Rf values, of 0.920 and 0.773, compared to benzoic acid and o-toluidine because it had the least hydrogen bonding ability to the silica gel, while o-toluidine and benzoic acid may have been able to form more hydrogen bonds to the polar gel. O-toluidine

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