Abstract: Ocimum blend/flavourant tea is most consumable breakfast beverage in India. It is very popular tea flavouring herb Ocimum sanctum known as "Tulsi" or "Holy Basil" in India, along with seven other species/cultivar dried under shed and in a natural convention cabinet drier at 40C, with various blanching treatments. Thermal (Deep freeze- liq. N2, dry-hot air and wet heat-boil H2O) and chemical (NaCl, NaHCO3 and CaOCl2) treatments studied for their effect on optimum antioxidant potential. The abundance of antioxidant constituents varied in different Ocimum spp/cultivars and follows the order- Caffeic acid (CA) and rosmarinic acid (RA) in O. sanctum-CIM Ayu (OSAy) and rutin in O. gratissimum [OG]. Blanching treatments severely degraded all the three major Ocimum phenolics. Surprisingly 77% increased CA content, …show more content…
Post-harvest blanching treatment and extraction The freshly harvested Ocimum aerial parts dried under shed and powdered. For the postharvest blanching, experiment samples of 5.0 gm of samples taken for each treatment. The following drying and blanching experimental performed: a) RT- dried at room temp for 5 days (No-blanching); b) Hot Air- without any treatment hot air drying at 40C for 5 days; c) Liq. N2- Shock freezing with liquid nitrogen followed by freeze drying (-80C) for 5 days; d) CaOCl2- dipped in CaOCl2 solution (2.5%, w/v) for 3 min followed by hot air drying at 40C for 5 days; e) NaHCO3- dipped in NaHCO3 solution (2.5%, w/v) for 3 min followed by hot air drying at 40C for 5 days f) NaCl - dipped in NaCl solution (2.5%, w/v) for 3 min followed by hot air drying at 40C for 5 days g) Boiling water- dipped in boiling water for 5 min followed by hot air drying at 40C for 5 days Relative humidity, temperature and weight loss monitored during drying process. After complete drying (constant weight) ,samples prepared for HPLC analysis using ultrasonic assisted extraction (UAE) method. 2.6. Free radical and antioxidant
On December 5, 2012, Daisy Luther, a journalist from Northern California wrote a blog entry on the conspiracy surrounding “certified organic” labels that is claimed by some companies and retailers. She brings up the question of whether these labels being stamped on food can really be verified or are they just a way to empty out the wallets of consumers. In the website The Organic Pepper, the blogger generally gives advice for different problems people encounter on a daily basis. Through her blog entries varying from ways to stay healthy to frugal living, Luther states her opinion of governmental interference on our food supply by citing sources from articles from Natural News and Time Magazine. She first starts out by arguing about how the
Method In this experiment, we tested our hypothesis through the use of chromatography paper. Chromatography paper have the ability to separate colored chemicals or substances. We also used 10 grams of grinded coleus leaves and grass leaves, 90% acetone, magnesium sulfate, pencil, ruler, forceps, 1 capillary, 1 jar with lid, and a timer to conduct this experiment. We began this experiment by grinding the 10 grams of coleus leaves and grass leaves in 10mL of 90% acetone.
In this lab, three unknown compounds were separated from a mixture and identified by melting point. Unknown mixture #124 has components of acid, base and neutral compound. The compounds were identified by melting point and matched up with the known melting points from a given list. In order to identify the compound it was important to separate by dissolving the mixture in an organic solvent which was not soluble in water, and then extracting the solution first with HCl, and then dilute sodium hydroxide solution. From the separation mixture, the aqueous layer were obtained and labeled as TT-1 (base), TT-2(acid) and TT-3 (neutral) in three different test tubes for later recovery.
Hence in the sample only copper (II) chloride will dissolve leaving the sodium chloride behind once filtered through. The copper (II) chloride can then be obtained by evaporating the methanol; which has a boiling point of 65 degrees celsius whilst copper (II) chloride has a boiling point of 993 degrees celsius, thus allowing the methanol to be easily evaporated out of the solution eliminating concerns of the copper (II) chloride evaporating alongside the methanol. Purpose To investigate different methods of separating copper (II) chloride and sodium chloride in order to obtain the original masses of both substances.
Claim: Through the tests performed in lab, it was concluded that unknown powder E was NaHCO₃, powder K was MgSO₄ and powder V was cornstarch. Evidence: Unknown element E was first tested with phenolphthalein and the solution turned pink, this test left 3 possible compounds; NaHCO₃, Na₂CO₃, and CaCO₃. Next the compound was tested with iodine and the solution turned yellow showing that now there was two compounds that could be the identity of unknown powder E; NaHCO₃ and CaCO₃. After that compound E was tested for being soluble in water, the powder was soluble, this proved that unknown element E is NaHCO₃. Unknown Element K was also first tested following the steps for the phenolphthalein test and the compound turned clear, this test ruled out NaHCO₃, Na₂CO₃, and CaCO₃. Second the unknown powder was tested with iodine and the solution turned yellow, leaving NaCl, CaSO₄ and MgSO₄ as possible identities of unknown powder K. Considering this it was next tested for solubility in H₂O, and it was concluded that the compound was soluble. Next the unknown element was tested with sodium hydroxide and a white precipitate formed proving that unknown powder K was MgSO₄ because NaCl and CaSO₄ were ruled out.
Vacuum filtration was performed on the crude product, then it was recrystallized for purification. Melting point analysis was conducted on the recrystallized product to determine its identity. 3. The three possible mechanisms in this experiment were syn-addition
Abstract: Gel electrophoresis is a method used to separate DNA fragments according to size. During our study we pondered on one particular question; whose blood was left at the crime scene in the AP biology classroom? Before carrying out our experiment we learned about the process of gel electrophoresis and the use restriction enzymes. After analyzing our results, we decided to reject our hypothesis because our experiment showed strong evidence against what we originally hypothesized.
First, two grams on an unknown white compound were given. The possible compounds the known could be were CaCO3, KNO3, NH4Cl, CaCl2, K2SO4, (NH4)2SO2, Ca(NO3)2, NaC2H2O2, K2CO3, MgCl2, Na2CO3, 0.1 M AgNO3, MgSO4, NaCl, 0.2 M BaCl2, KCl, NaSO4, Mg(s), HCl, HNO3, NaOH, HC2H3O2, H2SO4, and KOH. The solubility test required using a scale to measure .575 of our unknown white compound. The unknown compound was measured in a 100 mL beaker.
In this experiment, antioxidants and vitamins were involved. Antioxidants are molecules which can safely interact with free radicals and terminate the chain reaction before vital molecules are damaged. Although there are several enzyme systems within the body that scavenge free radicals, the principle micronutrient (vitamin) antioxidants are vitamin E, beta-carotene, and vitamin C. Additionally, selenium, a trace metal that is required for proper function of one of the body 's antioxidant enzyme systems, is sometimes included in this category. The body cannot manufacture these micronutrients so they must be supplied in the diet. Free radicals are atoms or groups of atoms with an odd (unpaired) number of electrons and can be formed when oxygen
In this lab, extraction was used as a method for separating a mixture of compounds. Specifically, Benzocaine and Benzoic Acid were individually isolated from a starting mixture which consisted of both components. During this lab, Benzocaine was isolated into crystals, while benzoic acid formed a white powdery solid. This was done by first isolating benzocaine. Once separated, this clear liquid had 6M of sodium hydroxide added to it and was put in an ice bath.
Experiment #1 Isolation of Caffeine from Tea Date: 11.09.14 Prepared by: Alibek Abilev Purpose The aim of the experiment was to isolate crude caffeine from tea leaves by using liquid – liquid extraction with methylene chloride, purify the crude substance by performing sublimation and determine the melting point of both crude and pure caffeine. Safety Lab coat, goggles, gloves. Methylene chloride is a carcinogenic substance, therefore should be kept in a well-ventilated place.
This type of chromatography is used for the analysis and purification of low to moderate molecular weight, thermally liable molecules. It is the most effective method for the separation of chiral compounds. Principles are similar to high performance liquid chromatography however supercritical fluid chromatography uses carbon dioxide as the mobile phase. The entire chromatographic flow path must be pressurized, because supercritical phase represents a state in which liquid and gas properties converge. Supercritical fluid chromatography brings the advantages and strong aspects of HPLC and GC
22.5 g of plate count agar powder was dissolved in a litre of sterile distilled water on the hot plate 2. pH of the solution was adjusted to 7.0 ± 0.2 by adding NaOH or HCl and was immediately transferred into the Schott bottle to be autoclaved at 121 ° C for 15 minutes 3. Prepared medium was stored in 4° C chiller Lauryl Sulphate Broth
⋅ 5H2O, which has about 36.0%, and CuCl2 ⋅5H20 (21.17%). Materials: Ring stand, ring clamp, evaporating dish, Bunsen burner, clay triangle, crucible tongs, electronic balance, sample of hydrated salt. Methods:
A comparative research study has give evident that cocoa have higher antioxidant activity than black tea, green tea, and red wine. iii. Antioxidants help to neutralize the oxygen-based free radicals that are present in the body. iv. Free radicals are associated with human disease, including cancer, atherosclerosis, Alzheimer 's disease, Parkinson 's disease and many others.