Wound Coating Research

This article presents the results of research on the effectiveness of wound coatings based on allogeneic cultured fibroblasts, keratinocytes and mesenchymal stromal cells of a diposetissueim mobilized on the collagen matrix. The experiment was conducted by inflicting wounds on the skin of rabbits and subsequent treatment with coatings. An ointment “Levomekol” was used as control.
The results showed that the wound coatings based on cultured fibroblasts and mesenchymal stromal cell of adipose tissue have a strong wound-healing and anti-inflammatory effect.
1. Introduction
The treatment of burns and surgical wounds is one of the urgent problems of modern combustiology and surgery.
Burns according to the World Health Organization rank third among

Hair with a diameter of 3cm was shaved from the lumbosacral region of the back of rabbits. This region was then treated with alcohol and 2% lidocaine was injected as local anesthesia. Then the skin with a diameter of 2 cm was excised and coatings with cells (test groups) or levomikol (1g of ointment contains 7.5 mg of chloramphenicol and 40 mg of methyluracil) ointment (control group) were applied. In test groups coatings were applied once, while in the control group the ointment was applied 3 times. At 4, 8 and 12 day of the experiment the diameter of the wound was measured at several points. On 12th day of the experiment the epithelialized skin on the wound was collected in the shape of triangle from the periphery to the center under local anesthesia. All of the animals survived after the

On the surface of the epidermis minor pieces of stratum corneum with exclusion zones are formed. Proliferation of basal layer and formation of new vessels can be seen in epidermis. Skin appendages and inflammatory infiltration around the vessels and in the course of connective tissue can be seen in skin dermis (Figure 7). Additionally zones with damaged epidermis in the form of pink structureless mass and partial detachment of the epidermis can be observed. Underneath in the dermis inflammatory infiltration is observed (Figure 8). Рисунок 7. Staining with hematoxylin and eosin, х100 Рисунок8. Staining with hematoxylin and eosin, х50

The morphological description of histological preparations shows that best treatment results were obtained in the first and third groups, where coverings based on cultured fibroblasts and MMSC were used. This is due to the high regenerative capacity of fibroblasts and multipotent mesenchymal stromal cells. Thus, the most promising preparation for the wound treatment is cultured fibroblasts coating. Isolation and culture of these cells is technologically easy and relatively inexpensive when compared to isolation and culture of keratinocytes and MMSC, as it does not require expensive growth media and