Figure 2. Column chromatography set-up After setting up the column, 2 10-ml of the chosen solvent was obtained and was placed in two separate test tubes. Using a dropper, ~0.5 mL of the food dye was put into the column by dropping it at the side of the column in a circular motion. The chosen solvent was then added just after the green food
Solubility: The solubility of the drug sample was carried out in different solvents (aqueous and organic) according to I.P. The results are then compared with those mentioned in the official books and Indian Pharmacopoeia. Melting point The melting point of Itraconazole was determined by capillary method using digital melting point apparatus. ANALYTICAL METHODS STANDARD CURVE Preparation of standard solution: 100mg of itraconazole was accurately weighed into 100ml volumetric flask and dissolved in small quantity of buffer. The volume was made with 6.8 pH Phosphate buffer to get a concentration of 1000µg/ml (SS-I).
Among heterocyclic compounds, 1,3,4-oxadiazole has become an important construction motif for the development of new drugs. Compounds containing 1,3,4-oxadiazole cores have a broad biological activity spectrum including antibacterial, antifungal, analgesic, anti-inflammatory, antiviral, anticancer, antihypertensive, anticonvulsant, and
50mL HCl was added to the calorimeter, which was covered with the lid and inserted with a probe. Readings were collected and then 50mL NH4OH was swiftly added to the solution, with the stir bar mixing the reaction constantly. The initial and max temperature temperatures were recorded and the change in temperature was recorded. The data was saved onto the USB. Regarding reaction 4, the duration was changed to 480 seconds.
At this point, the change in pH with respect to volume was minimal since these values were far from the equivalence point, which occurred experimentally at 27.41 mL. This can also be seen on the graph as the plateau before the inflection point occured. To calculate the Ka of the acid, the following formula is
Then the drug content was determined by comparing the absorbance of this solution with standard solution having same concentration. 6.4.8. In vitro drug release studies98: In-vitro release of zolpidem tartarte from pellet formulations was investigated by the Paddle method (Apparatus II). The dissolution medium was 500 mL of 0.01N HCl solution at 37 ± 0.5 °C and the rotating speed was 50 rpm. At certain time intervals, 1 ml of sample was withdrawn and immediately same amount of fresh medium (37±0.50C) was replaced.
After that with further increase in adsorbent dosage there are no significantly changes seen in percentage removal. Graph shown below represents the maximum percentage of removal of chromium when the initial concentration of chromium in aqueous solution is 30mg/l. Graph 6.8: % removal of Chromium when initial Chromium(VI) concentration is 30mg/l 6.5 FINAL EQUILIBRIUM OPERATING CONDITIONS FOR MAXIMUM REMOVAL OF CHROMIUM(VI) Table 6.4 PARAMETER Values investigated pH 2 Temeperature 32oC Contact time 4hrs 6.6 ADSORPTION
0.5 grams of NaCl was added, stirred and filtered. The residue was washed with 2M HCl until the volume of the filtrate became 6mL. 1mL of the filtrate was tested with 2-3 drops of Mayer’s reagent, Wagner’s reagent and Draggendorrf’s reagent. The relative amount of precipitation was observed: (+ Slight turbidity), (++ Definite turbidity), (+++ Heavy turbidity). 3.2 Alkaloidal Analysis
1.1 Procedure To determine the percentage of Calcium carbonate (CaCO3 ) in a given toothpaste sample, containing CaCO3,back titration is carried out using 0.16M of hydrochloric acid(HCl) and 0.08M of sodium hydroxide (NaOH). First, 10ml of standard HCI solution was drawn using a transfer pipette. Certify that the meniscus was read correctly. The drawn solution of 0.16M of standard HCl was then pipetted into a clean conical flask, that was prepared by washing with distilled water. Any droplets of HCI suspending from the pipette tip was removed by tapping against the inner walls of mouth of the conical flask.
 Appropriate concentrations of SBP hydrolysate were mixed 1: 4 (v/v) with 200 M DPPH solution in anhydrous methanol for 30 min in the dark. The absorbance of the samples was recorded spectrophotometrically on a microplate reader (Multiskan Go, Thermo Fisher Scientific) at 517 nm. A control group containing DPPH solution without sample was also prepared. Ascorbic acid was used as a positive control. ABTS radical scavenging