Oral Rinse Lab Report

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Firstly 1 screwcap tube containing 200 µl Instagene matrix,1.5 ml microcentrifugetube and a cup containing 10 ml of 0.9% saline solution were supplied by instructors and the saline solution was poured from the cup into the mouth by two students from the group and the saline was rinsed vigorously for 30 seconds and than the saline was expelled into the cup.Cheek cells were used.1ml of the oral rinse was transfered into the ependorf tube by the help of a pipet.The ependorf tube was centrifuged for 10 minutes.The pellet should be observed at the bottom of the ependorf tube.After, the supernatant and discard were carefully poured off(not to lose the pellet).The pellet was was resuspended throughly by vortexing until cells clumps remain.By using a micropipet 200 µl of resuspended cells were transfered into this screwcap tube, which is included with Insta Gene Matrix.The caps were screwed tightly and shaked well for 10 minutes at 56°C.The tubes were incubated.The incubation’s degree was increased by the instructor not to wait until next lab session.After incubation,the samples were centrifuged for 10 minutes.Than 20µl of PCR master mix was added into the PCR tube (The colour of the master mix was yellow in group 3) and 20 µl of DNA template from the supernatant was added into the PCR tube.Later, by pipetting up and down 2-3 times two of the

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