Firstly 1 screwcap tube containing 200 µl Instagene matrix,1.5 ml microcentrifugetube and a cup containing 10 ml of 0.9% saline solution were supplied by instructors and the saline solution was poured from the cup into the mouth by two students from the group and the saline was rinsed vigorously for 30 seconds and than the saline was expelled into the cup.Cheek cells were used.1ml of the oral rinse was transfered into the ependorf tube by the help of a pipet.The ependorf tube was centrifuged for 10 minutes.The pellet should be observed at the bottom of the ependorf tube.After, the supernatant and discard were carefully poured off(not to lose the pellet).The pellet was was resuspended throughly by vortexing until cells clumps remain.By using a micropipet 200 µl of resuspended cells were transfered into this screwcap tube, which is included with Insta Gene Matrix.The caps were screwed tightly and shaked well for 10 minutes at 56°C.The tubes were incubated.The incubation’s degree was increased by the instructor not to wait until next lab session.After incubation,the samples were centrifuged for 10 minutes.Than 20µl of PCR master mix was added into the PCR tube (The colour of the master mix was yellow in group 3) and 20 µl of DNA template from the supernatant was added into the PCR tube.Later, by pipetting up and down 2-3 times two of the
We are doing a gummy bear lab. Gummy bears come in different sizes, shapes, and colors. Gummy bears are squishy, chewy, and sticky, they are made of sugar which are glucose and glucose are carbohydrate. We predict that during this stage of lab the solute and solvent will go through the stages of hypertonic, hypotonic and isotonic. We get 4 gummy bears and 4 cups filled half way with different types of liquid, such as Salt-Water, Coffee-Creamer, Vinegar, and Soda.
First, label one micro centrifuge tube +pGLO and another –pGLO. Using a sterile transfer pipet, transfer 250µl of competent cells (E. coli + CaCl2) into each tube and place them in crushed ice. Examine the pGLO plasmid DNA solution with the UV light and note your observations. Pipet 10µl of pGLO plasmid into the +pGLO tube and mix, close and return it to the ice rack. Do NOT add plasmid DNA to the –pGLO tube.
Purpose: To identify an unknown microorganism by performing a series of biochemical tests on a pure bacterial culture. Materials and Methods: Tests: Lactose fermentation: Fermentation makes energy available for use by microorganisms by anaerobic breakdown of carbohydrates. The product can either be an acid or gas. When it is positive, the broth will turn from red to yellow and if gas is present a bubble is formed.
Using two separate aseptic pipettes, 250 µl of LB broth were added to each micro test tube and mixed gently. Likewise, using two separate, aseptic pipettes for each tube, 100 µl of solution was added to the appropriate agar plate. After, using a new loop for each plate, the solution was spread gently across their surfaces. Lastly, the plates were stacked, taped together, and labelled before placing them upside down in an incubator set at 37°C
Unknown Lab Report Unknown # 25 By: Jenna Riordan March 19, 2018 Bio 2843 1. Introduction Microbiology is the study of microorganisms found in all different environments throughout Earth, from the hot thermal vents at the bottom of the ocean to the ice at the top of a mountain.
ST Report In the experiment, the problem was the contaminants that were affecting the quality of the water samples. To fix this issue, three scientists had to determine the contaminants that were present in the samples. One sample was from the school sink and the second sample was from an unknown source. The scientists conducted many tests to figure out what pollutants were present in the water.
The gummy bear's mass and volume will increase while the density of the gummy bear would decrease after it is put into water overnight. (#)This lab experimented to figure out wah changes would take to the gummy bear’s mass, volume, and density after sitting in a cup of water overnight. To do this the gummy bear's dimensions and weight was taken on the first day, along with its density and then the gummy bear was placed and water. When the gummy bear was taken out of the water on day two, the dimensions, weight, and density were taken again, and the difference between the two days was found. (#1)
Once the program was run, the machine began its thirty-five PCR cycles. Each step in these three step cycles is defined by a specific time span and temperature. However, before the first cycle begins, initial DNA denaturation occurred for five minutes at a temperature of 95 oC in order to ensure that all the DNA had become single-stranded. After this initial denaturation, the thirty-five PCR cycles could begin, first with thirty seconds of DNA denaturation at 95 oC, then followed by a drop of temperature to 58 oC for thirty seconds for the primers to anneal to its target sequence on the template DNA, and finally thirty seconds at 72 oC for primer extension in which DNA synthesis is carried out by Taq Polymerase. After the thirty-five cycles were complete, there was an additional five minutes at 72 oC for the final extension of the DNA to ensure that all synthesis had been completed.
The purpose of this experiment was to observe the relationship between different solutions and their effect on the mass and length of a gummy bear. Gummy bears with the same relative size and colour were placed in 50 mL beakers with the same amount of their designated liquid. The different solutions studied were water, oil, vinegar, a simple sugar solution, and rubbing alcohol. In the end, based on the observations made, the majority of the hypotheses created were not proven to be correct.
The objective of the sludge lab was to determine how many different pure substances were in the sludge by using the methods and techniques we have learned throughout the year. We had to pick separation methods so we could separate our sludge and then test characteristic properties on our separated liquids and solids. This experiment made us use our knowledge on characteristic properties to pick the ones we should test to help us identify our pure substances. Characteristic properties are properties that help identify a solid or liquid. Each solid or liquid has a certain density, boiling point, solubility, flammability, so if you know what each one is then you can use that information to help you identify your solid or liquid.
We then obsevered the two slides for number of cells as well as for food vacuoles inside a cell using a microscope at times of 0,5,10,20, and 30 minutes. Results The following graphs show the results of this experiment. The tetrahymena sample that was introduced to concentrated tobacco had a lower cell/vacuole ratio than the tetrahymena sample that was not exposed to
A pipette of 1.5 microliters of the overnight bacteria culture was labeled A and the tube was then transferred to a micro centrifuge tube. The overnight bacteria were labeled B and put into a second micro centrifuge. The cells
How does the amount of baking soda mixed with vinegar affect the volume of gas produced? The rate of reaction is the increase or decrease time taken at which the products are formed or concentration increase or decrease between a reaction of two or more substances. In the reaction, new bonds are formed whilst others have been broken.
It also better to ensure that the materials like hemocytometer and pipettes are sterilized and clean so that there would be lesser or no artifacts would be seen under the microscope. The researcher was to use trypan blue exclusion method to test for cell viability, observe the non-viable and viable cells, and was able to have a cell count using the
Abstract — This experiment was conducted to familiarize the students with the procedures regarding distillation—to be more precise, the separation of ethanol from an alcoholic beverage—using a distillation set-up consisting of boiling chips, a Bunsen burner, a condenser, a thermometer and several other materials. In the end, it was discovered that one may actually separate a homogeneous mixture, given that the components of said mixture differ in volatility and that they utilize a complete distillation set-up and follow laboratory safety rules and regulations. Keywords — Matter, homogeneous and hetereogeneous mixtures, distillation, volatility, boiling point I. INTRODUCTION There are typically two categories of matter, these are pure substances