Then five millilitres of sample “A” was placed in the test tube labeled “A”. This was then repeated with the next three samples. 20 drops of Biuret reagent were then added to each test tube. The colour of each test tube was recorded and if proteins were present that was recorded for each test tube. Finally, the pH was recorded for each sample using pH
MDA level was determined by thiobarbituric acid reactive substances (TBARS) in serum, based on the reaction between MDA and TBARS. Standard Malondialdehyde solution in 5 mL of volume was processed along with test samples. 1.5 mL of 0.8% of TBA was added to 1 mL of the serum sample. Then 0.4 mL of 8.1% sodium dodecyl sulphate and 1.5 mL of acetic acid was added. The mixture was finally made upto 5 mL with distilled water and placed in hot water bath at 95ºC for 1 h. After cooling, 1 mL of distilled water and 5 mL of the mixture of n-butanol and pyridine (15:1, v/v) was added.
The final volume was recorded. A pH probe connected through Microlab was calibrated using buffer solutions of pH 4.00, 7.00, and 10.00. The calibrated pH probe was used in order to measure the pH of the titrated solution of the unknown weak acid. These same steps were repeated except 2 mL of the strong base were titrated into the weak acid solution instead of 4 mL. This process was repeated 10 times.
During surgery, the infusion rates were adjusted at 10-15 ml/kg/h to maintain values of systolic arterial blood pressure and heart rate within ± 20% of baseline values. After induction of anesthesia al6-French gauge multi-orifice nasogastric tube was inserted and its correct position was confirmed by epigastric auscultation of injected air. Gastric content measurement (volume and pH) was made: 5min after induction of
Guinea pig ileum was selected as tissue type and Acetyl choline as agonists. Then different amount of acetylcholine were added to the organ bath which produced different results and finally, a log – dose response curve for acetyl- choline was constructed with the X axis corresponding to drug dose (Drug concentration) and the Y axis corresponding to response (gms). Then we calculated for (ED50) and (ECR) with the help of the graph. RESULTS FOR ACETYLCHOLINE AND NON
50mL HCl was added to the calorimeter, which was covered with the lid and inserted with a probe. Readings were collected and then 50mL NH4OH was swiftly added to the solution, with the stir bar mixing the reaction constantly. The initial and max temperature temperatures were recorded and the change in temperature was recorded. The data was saved onto the USB. Regarding reaction 4, the duration was changed to 480 seconds.
The sunflower seed is the seed of the sunflower (Helianthus annuus). The methanol extract of seeds of Helianthus annuus were screened for analgesic activity in mice model to systematically explore the medicinal values of the plant. Acetic acid induced writhing and hot plate methods were used to confirm the central and peripheral analgesic action. The extract at dose 100mg/kg and 200mg/kg showed attenuated writhing inhibition at 50.35% and 57.85% in case of acetic acid-induced writhing test which showed significant analgesic activity (p< 0.05). In the hot plate method increase (p < 0.05) of latency period was also observed in comparison to standard aspirin.
SDS-Polyacrylamide gels were prepared and the glass plates were washed with 70% ethanol and water. After drying the plates, water was used for test leakages. Two SDS-Polyacrylamide gels were prepared according to the following recipe. These all above components of the running gel were added in a 50 ml tube and solutions were mixed and pipetted into the prepared gel chambers. Glass plates were filled ¾ and the gel was covered with 100-500 µl Isopropanol in order to achieve an even surface.
After testing the burret reading, the next step was to start the experiment by preparing approximately 0.1 M NaOH solution. First calculate the amount of 6.0 M NaOH stock solution needed to prepare 500 mL of 0.1 M NaOH solution. A 10.00-mL graduated cylinder was used to measure the the calculated amount of 6.0 M NaOH, and then filled to the top with deionized water. The 10.00 mL was then poured into a 500 mL plastic bottle. The 10.00 mL graduated cylinder was refilled with deionized water and was poured into the same 500 mL plastic bottle.