P-Nitrophenol Reaction Lab Report
In this experiment, absorbance at A400 was directly proportional to the number of moles of p-nitrophenol. This shows that there is a linear relationship between the absorbance and the number of moles of p-nitrophenol, which later also shows that there is a linear relationship between the absorbance and the concentration of the p-nitrophenol, as the number of moles of p-nitrophenol will increased when the concentration of p-nitrophenol increased. The R2 value obtained for the standard curve is 0.9997, which indicates that the data obtained fit the line of best fit, showing that the data are accurate. The values of absorbance per µmol of p-nitrophenol were then obtained from the gradient of the curve, which will later be used in calculation in determining the enzyme activity. Based on Graph 3, it was shown that the enzyme activity of alkaline phosphatase increased with the increase of MgCl2 …show more content…
The alkaline phosphatase has a lower enzymatic activity when pH 7 and 7.5 Tris-HCl were used; whereas the enzyme has a higher enzymatic activity when pH 8, 8.5 and 9 Tris-HCl were used, with the enzymatic activity at pH 9 being the highest. This shows that the alkaline phosphatase works optimally at alkaline condition such as pH 9 (Kaslow, n.d). The results of the graph show that alkaline phosphatase work less optimally at lower pH, this may due to at lower pH, the positively-charged amino side chain of the peptide sequence of alkaline phosphatase will release the hydrogen ions, therefore causing the tertiary structure of the enzyme altered, which will causes conformation change in alkaline phosphatase’s active site. Thus, the substrate p-nitrophenyl phosphate will experience some difficulties to bind to the binding site on active site of alkaline phosphatase, therefore decreasing the enzyme activity when pH becomes
Chem 100 Final Lab Report
1366 Words | 6 PagesCadet Eric Wiggins Date: 18 September 2014 Course Name: Chem 100 Instructor: Captain Zuniga Section: M3A Identification of a Copper Mineral Intro Minerals are elements or compounds that are created in the Earth by geological processes. The method of isolating metals in a compound mineral is normally conducted through two processes.
Peroxidase Chemical Reaction Lab
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Pglo Lab Report
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Turnip Peroxidase Experiment
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Horseradish Peroxidase Lab Report
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Electrophilic Aromatic Substitution Reaction Lab Report
821 Words | 4 PagesThe purpose of this experiment was to learn about the electrophilic aromatic substitution reactions that take place on benzene, and how the presence of substituents in the ring affect the orientation of the incoming electrophile. Using acetanilide, as the starting material, glacial acetic acid, sulfuric acid, and nitric acid were mixed and stirred to produce p-nitroacetanilide. In a 125 mL Erlenmeyer flask, 3.305 g of acetanilide were allowed to mix with 5.0 mL of glacial acetic acid. This mixture was warmed in a hot plate with constantly stirring at a lukewarm temperature so as to avoid excess heating. If this happens, the mixture boils and it would be necessary to start the experiment all over again.
Tetraphenylnaphthalene Lab Report
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Turnip Peroxidase Lab Report
2136 Words | 9 PagesAlthough it was expected for water to be the optimal pH, it was also assumed that more drastic activity would happen with the other pH’s. For example, it was thought that it would still have some noticeable increase; however, when looking at the data and the graph, the numbers oscillate with no noticeable positive or negative trend. Tables 1 and 2 show that the absorbance rate in comparison to the absorbance rate in Table 3 are significantly smaller. Furthermore, after calculating the processed data for reaction rates and looking at the graph, pH 7 water had the highest rate. This experiment gives a good insight for future references about enzymes and the effect of environmental factors and its functions.
Enzyme Turnip Peroxidase Lab Report
1117 Words | 5 PagesThe effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate.
B-Galactosidase Lab Report
1764 Words | 8 Pages1.1 Abstract The purpose of quantitative analysis of protein using a spectrophotometer is to measure the concentration of proteins in a given sample. The experiment is conducted by laboratory method (Biuret Test) and using spectrophotometer to analyze the absorbance of reactants at 540 nm, hence determining the concentration of the proteins in a given sample. The purpose of stopped enzyme assay to study B-galactosidase is to determine the effect of temperature and concentrations of substrate on enzyme activity.
Bio Chem: Lab Report Of Amylase In Enzyme
916 Words | 4 PagesIn this experiment , we can prove that the temperature, pH and salt are the factors that will affect the structure and function of the enzyme as it is a kind of protein . Therefore, there may be an influence on the activity of enzyme which substrates cannot be binded on the active site if the amylase in too high or low ph and temperature and excess salt environment . On the other hand optimum ph and temperature and suitable salt concentration may favour the amylase activity . Reference : 1.2016, May 08). Effects of pH on Amylase Activity.
Alkaline Phosphatase Enzyme Lab Report
2575 Words | 11 PagesIntroduction 1.1 Aim: To determine the kinetic parameters, Vmax and Km, of the alkaline phosphatase enzyme through the determination of the optimum pH and temperature. 1.2 Theory and Principles (General Background): Enzymes are highly specific protein catalysts that are utilised in chemical reactions in biological systems.1 Enzymes, being catalysts, decrease the activation energy required to convert substrates to products. They do this by attaching to the substrate to form an intermediate; the substrate binds to the active site of the enzyme. Then, another or the same enzyme reacts with the intermediate to form the final product.2 The rate of enzyme-catalysed reactions is influenced by different environmental conditions, such as: concentration
Michaelis Menten Model Lab Report
943 Words | 4 PagesThe results in this experiment were used to study the effects of enzyme concentration, inhibitor presence and substrate concentration in a biochemical reaction. The enzyme and substrate concentrations were calculated in part 1 along with the Vmax, Km and Ki in part 2 to understand the influence of these factors on the hydrolysis reaction of 4-nitrophenylphosphate and biochemical reactions in general
Effect Of Enzyme Concentration On Enzyme Reaction
1046 Words | 5 PagesThe 3 concentrations of enzymes were 0.5 ml, 1.0 ml, and 2.0 ml of turnip extract, while the substrate consisted of 0.1ml, 0.2 ml, and 0.4 ml of hydrogen peroxide. In a separate tube, the control was made up of turnip extract and guaiacol, known as the color reagent. This was recorded the absorbance every 20 seconds for 3 minutes.
Phenol Concentration Lab Report
1403 Words | 6 PagesThe initial phenol concentration provides an important driving force to overcome all mass transfer limitations of phenol between the aqueous and sorbent phases. Therefore a higher opening phenol concentration will enrich the biosorption process. The increase in sorption capacity of bacterial strain with an increase in the initial phenol concentration might be due to a higher probability of collision between the phenol molecules and biosorbent. With increase in phenol concentration from 100 to 500 mg/L, the percentage removal reached 99% in 2 days. The equilibrium
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