2.Molecular studies in palm callus cultures
A. Protein analysis:
Electrophoreses protein analysis was carried out to study the similarity of the genetic at different time of Laser irradiation for cultures of date palm callus. 42.5 % Monomorphic and 7 total band had bean found Figure 1. Molecular weight 29 KDa was not found in the callus treated for 5,10 and 15 min, While Molecular weight 13 KDa had not been appear at in 10 and 15 min. Molecular weight 20 KDa was not showed at 10 min (Figure 1).
Fig(1)
B. DNA analysis:
DNA analysis was obtained by using five primers for the similarities between the time of Laser irradiation for palm callus. Figure 3 showed that primer HB15 for Molecular weight of (140 and
…show more content…
and Clark, M. S. (1997). The application of biotechnology to date palm culture. Plant Biotechnology and plant Genetic Resources for sustainability and productivity, edited by Kazuo and Watanabe and Eija pehu, R.G. , Landes company, chapter. 14, 183-195.
3-Chen Rumin, Li Nianghui, and Gdast Xu Xianghao, (1992). Laser effects on callus growth and differentionation of carnation, Journal of South China Agricultural University.1.
4-Corner, D. J. H. (1996).The natural history of palms. University of California Press,Berkeleley and Los Angeles PP.1-393.
5-Dubois, M., K.A. Gilles, J.K. Hamitton, P.A. Rebers and F. Smith, (1956). colorimetric for determination of sugar and relateol substances. anal .chem, 28: 350 -356.
6-Kurata, H., T. Achioku, N. Okuda and S. Furusaki, (1998). Intermittent light irradiation with a second scale interval enhances caffeine Production by Coffea arabica cells. Biotechol. Prog., 14: 797-799.
7-Laemmli, U.K., (1970). Cheavage of structural proteins during the assembly of the head of bacteriophage T4. Nature., 227: 680-685.
8-Larsen, P., A. Harbo, D.F. Klungsour and T. Asheim, (1962). On the biogenesis of some indole compounds in Acetobacter xylnum. Physiol. Plant., 15:
…show more content…
Bailey and R. Baum, (1977). Modification of a CTAB DNA extraction protocol for plants containing high polysacehaside and polyphenol components. Plant Mol. Biol. Report, 15(1): 8-15
12-Rosen, H., (1957). Amodifiedninhydrin colorimetric analysis for aci Arch. Biochem. Biophys, 67: 10-15.
13-Salyaev, R.K., Dudareva, L.V., Lankevich, S.V., and Sumtsova, V.M. (2001), The Effect of Low-Intensity Coherent Radiation on the Processes of Morphogenesis in the Wheat Callus Culture, Dokl. Akad. Nauk, vol. 376,pp. 830–832.
14-Salyaev, R.K., Dudareva, L.V., Lankevich, S.V., and Sumtsova, V.M. (2001), The Effect of Low-Intensity Coherent Radiation on the Callus Formation in the Wild Cereals, Dokl. Akad. Nauk, vol. 379, pp. 819–820.
15-Salyaev, R.K., Dudareva, L.V., Lankevich, S.V., and Sumtsova, V.M. (2003), Effect of Low-Intensity Laser Radiation on the Lipid Peroxidation in Wheat Callus Culture, Russian Journal of Plant Physiology, Vol. 50, No. 4, pp. 498–500.
16-Wang Mansi; Li Mingdi; Cao Riqiang; Yang Zhengju; Xu Ziran; and Lu Mu, (1994). EFFECT OF He-Ne LASER AND MAGNETIC FIELD IN ONOSMA PANICULATUM B. et F.CALLUS CULATURE, Journal of Nanjing University (Natural Sciences).
First test, Morphological of Unknown consists of multiple of subtests. First subtest was used to determine the optimum temperature of unknown #398 growth by inoculation into 2 nutrient agar slants.
Method In this experiment, we tested our hypothesis through the use of chromatography paper. Chromatography paper have the ability to separate colored chemicals or substances. We also used 10 grams of grinded coleus leaves and grass leaves, 90% acetone, magnesium sulfate, pencil, ruler, forceps, 1 capillary, 1 jar with lid, and a timer to conduct this experiment. We began this experiment by grinding the 10 grams of coleus leaves and grass leaves in 10mL of 90% acetone.
Loren Eiseley put in a personal story of seeds exploding on his carpet. “A plant, a fixed, rooted thing, immobilized in a single spot, had devised a way of propelling its offspring across open space.” It made sense that flowers could not just rely on interaction with animals. The sound was so loud, it woke him up in the middle of the night!
In order to do this a PCR was set up. A PCR tube was obtained from the instructor. The PCR bead was then dissolved in 22 microliters primer cocktail. After the PCR bead was dissolved, three microliters of the DNA extracted from the first part of the experiment were added to the PCR mixture. This PCR mixture and the 1.5 ml extracted DNA samples were then submitted to the
An Investigation of photosynthesis for silver beet. The aim of this investigation is to find the average rate of photosynthesis for silver beet compared to gold beet. Methods DPIP is a measure to help find the rate of photosynthesis. The rate was calculated using the slope of the absorbance/time graphs Appendix a.
An aspirator was used to suck each Callosobruchus maculatus out of the large plastic tube in order to insert each of them into the petri dishes. Procedure: In order to carry out this experiment two different sized lima beans were needed. Two plastic containers that have plastic dividers were utilized to split the container into four sections. The two plastic containers were divided to which one contains which bean size.
Because carbon dioxide is absorbed by the plant during photosynthesis less carbon dioxide present in the chamber is a sign that photosynthesis is working. The four lights used for this experiment range across the light spectrum on both sides in order to test a wider variety of wavelengths. All lights will be placed directly on the spinach leaf at the same distance so as not to give any spinach leaf a different light intensity, which could affect the data. This experiment will be able to show which light, ranging across the light spectrum, will allow the Spinach to perform photosynthesis more efficiently.
This experiment is testing weather daisy seeds will grow faster in artificial light or sunlight .The purpose of this experiment is to see if the difference of light will affect the germination of a daisy. These pages of research will be explaining what this whole project means. From what soil will be in the daisies to what type of watt will be used in the lightbulb. This experiment is being tested because it is interesting to experiment the works of nature vs. man made products.
LABORATORY REPORT EXERCISE #5 INTRODUCTION TO THE COMPOUND LIGHT MICROSCOPE, PLANT AND ANIMAL CELLS Name_______________________________Section_____Teacher______________Date________ PRE-LAB QUESTIONS - answer the following questions using your textbook and valid internet sources. Be sure to cite your sources at the end of the prelab. You can type your answers to all questions except #1 and #9 directly into this document and then submit via Canvas. Type the answers for #1 and #9 at the end of the document. 1.
We then obsevered the two slides for number of cells as well as for food vacuoles inside a cell using a microscope at times of 0,5,10,20, and 30 minutes. Results The following graphs show the results of this experiment. The tetrahymena sample that was introduced to concentrated tobacco had a lower cell/vacuole ratio than the tetrahymena sample that was not exposed to
The objective of this study was to test the phototactic response of Daphnia when exposed to red (>600 nm) and white light. A 30 x 2 cm clear acrylic mesocosms with a 10 cm counting area was filled with distilled water and 10 Daphnia. We counted the number of Daphnia that traveled to the lit counting area after 10 minutes. There were twice as many Daphnia in the lit counting area for the control (white light) compared to the experimental group (red light). The results showed that red light had a negative effect on the phototaxis of Daphnia.
Aim To observe the effect of light intensity on the rate of photosynthesis. Hypothesis Light intensity is directly proportional to the rate of photosynthesis. When the light isn’t intense, not many oxygen bubbles will be produced and thus observed. This indicates that the plant will not have enough derived energy from the sun to activate photosynthesis. Whereas, when the light intensity is great, the rate of photosynthesis will be high.
Abstract In this experiment, the isolation, characterization, and determination of concentration and purity of deoxyribonucleic acid or DNA from Allium Cepa or onion was performed. DNA was isolated through the use of a homogenizing solution. The absorbance ratio was 1.5, which indicates protein contamination. Moreover, the characterization of its components was conducted through the use of different chemical tests.
Further experiments can be performed to improve the yield of DNA and to investigate the genetic material of different
Cell number two is microwaved for fifteen seconds. While cell number three will be microwaved for thirty seconds. Whichever plant sprouts first will show the results of if radiation affected the plants or not. ” Radiation is energy given off in the form of waves or tiny particles of matter.”