We did this by putting two dots on a piece of filter paper and placing it in either water or methylated spirits and to observe what happened and to see if any of the groups matched the mystery inks colour pattern. The two major areas of the analytical sciences are Spectroscopy and Chromatography. Spectroscopy covers a wide range of techniques for chemical analysis at the atomic and molecular level. Chromatography is a general term for techniques that
Leah Romero 10/30/2017 Conclusion Lab 3 Chem 102L In lab 3, fundamentals of chromatography, the purpose was to examine how components of mixtures can be separated by taking advantage of different in physical properties. A huge process in this lab was paper chromatography, which was used to isolate food dyes that are found in different drink mixes. The different chromatograms of FD&C dyes were compared to identify which dyes are present in each of the mixes. Chromatograms where made for the known FD&C and for the three Kool-Aid samples. The retention factor for each dye was calculated.
Jessica Vandeventer 23 March 2016 Quantitative Spectroscope and Visible Light Purpose/Question- The purpose of the lab is to build a diffraction grating spectroscope, and to view different lights. We also are going to draw the light spectra of the various light sources. Hypothesis– Each light sources will have a different spectral, but there will be some similarities in the different light sources. Data Tables/ Graphs – Analysis Questions- Exercise 1: Building and Calibrating a Spectroscope Questions A. Hold the grating several inches from your face, at an angle.
The iodine test determines the presence of starch in biological materials. It is predicted that, if starch is not present, the solution with iodine remains yellow. However, if starch is present the solution with iodine becomes a blue-black colour. Plants have starch as the storage polysaccharide (glucose units held together by glycosidic bonds) while animals have the equivalent of glycogen. In this experiment, the dark blue colour is visible because of the helical amylose and amylopectin reacting with iodine (Travers et al., 2002).
Each hair color is composed of different chemical compounds and also react differently than one another. Hair covers nearly every part of our body. In our hair, there are pigments called melanins. Melanins are broken down to either eumelanin and pheomelanin. The two key differences are that pheomelanin causes colors in the range of blond to red as eumelanin causes shades from brown to black.
Today 's dyes are also different they are often made by chemists. The chemist can do that because of the technology we have now, we can understand how the dye reacts with the molecules inside the different materials. Also the chemist can design dyes that don’t fade away or come off in the washing machine. In addition to what type of material they are made from, another way to categorize fibers is by their chemistry. Cotton and linen fibers are cellulose-based.
The main purpose for this experiment was to become familiar with the differences of elements, mixtures and compounds. The lab consisted on performing four different tests on both Iron (Fe) and Sulfur (S). If the four experiments were performed on each element then their would be a different reaction for each. The first test consisted of simply laying out a certain amount of each element of a piece of paper and observing the reaction when a magnet was placed on the bottom. The second test consisted on mixing in a 100ml beaker, 30ml of water and the respective amount used in the previous step of each element and analysing what the reaction was.
Collecting small fractions is essential in column chromatography because they can be combined together; large fractions can lead to multiple compounds in a specific fraction. The purpose of this experiment was to isolate the three components of Excedrin using column chromatography. Thin layer chromatography (TLC) was used to determine when each of the components had been fully eluted from the column. If there was an overlap in fractions between two components, liquid- liquid extraction was done to separate them. The compounds were characterized via NMR instrumentation and the percent recovery for each compound was calculated to determine whether the isolation was
Each of the analyte will have its own Rf value under certain circumstances. The separation of the phospholipid classes can be improved by two-dimensional chromatography. This technique requires developing the TLC plate in a direction, then dried, and developed in a solvent mixture at a 90 ° the first development (Singh and Jiang,