Paper Chromatography Lab Report
Question:
Describe what happened to the original spot of plant pigment extract? The spot traveled from the faint line on at the tip of the chromatography paper and it then created bands of colors. The original color of the plant extract was a black color and the once it was immersed in developer four it then started to travel up the paper strip.
How do your Rf values compare with those of your classmates? They were similar but some were different due to errors in the experiment.
List some other uses of chromatography? Chromatography can be used to separate pigments in fruit juices or writing ink.
Which of the four pigments migrated farthest and why? The yellow pigment migrated further because of carotene in the pigment.
Which of the two chlorophyll forms is more soluble? Chlorophyll B is more soluble because the
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Draw a faint line a few millimeters above the tip of the chromatography paper, at the center of the faint line use a micropipette to apply a pigment spot. Next, add 5 mL of developer one ( water) into the reaction chamber. After adding the 5 mL of developer one into the chamber then place on the tip of the chromatography tip into the solvent. It is very important to not allow the chromatography strip to be against the walls of the chambers or do not allow the chromatography paper strip get wet above the faint line. To keep the chromatography paper strip from dropping into the solvent of touching the walls you can fold the paper outside the chamber by using the cap to hold it is in the place. After eight to ten minutes of the chromatography, paper strip is in the chamber the pigment and solvent will start to travel up the paper strip, make sure to allow the paper to dry before getting results. Repeat the steps above but instead use two separate reaction chambers and add in separate tubes developer two (Isopropyl) and developer three (Isopropyl and
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