The plasmid needs to be isolated from the bacterial cells and the technique used is called alkaline lysis. This technique plays around with the pH to extract plasmid DNA. The culture is grown in medium containing ampicillin to select E.coli that have ampicillin resistance gene as their selectable marker. The addition of detergent to the cells causes cell lysis. The detergent attaches to the cell membrane and capture the protein and lipids of the cell membrane causing the cell to rupture.
Arthur Miller tries to explain the Salem Witch Trials through this novel and the movie, by giving detailed scripts to put an imaginable thought into the readers mind. They can keep up with the novel by being able to picture how it goes or how they wish for it to go. An article also states that, "...more than 250 years later- that Massachusetts formally apologized for the events of 1692" (Blumberg). The readers may have various opinions on what The Crucible is trying to explain throughout the book. Not all of the readers may be able to relate real-life situations to the story, which is a reason that writing this could put a different view in someone's
In Arthur Miller’s play The Crucible, Mary Warren, Abigail Williams, and Tituba, a slave, are instrumental in spreading the hysteria that resulted in the Salem Witch Trials. Throughout the play, Miller based the plot of his play on historical events and his characters show how paranoia and fear can escalate. Abigail Williams has a good sense of how to manipulate others and gain control over them. All these things add up to make her an awesome antagonist. She accuses Tituba for conjuring spirits which causes mass hysteria that blinded the people of Salem from the truth which is disregarded through the domino effect of accusations, the destruction of Salem, and death as the result.
Telling the truth may seem like the right path to take, but in the Puritans’ society it leads to nothing but consequences. In Arthur Miller’s play, The Crucible, reasoning and logic play a huge role in the society’s fear and paranoia. Proctor, Hale, and Giles are the main characters who have reasonable explanations for the chaos that has occurred. John Proctor is one of few characters that maintains valid logic towards the people’s fear and paranoia. In the beginning, Proctor gets in a dispute over whether Tituba, Sarah, and numerous others have dealt with the devil or not.
Is disobedience against the nation’s government needed if it means living to see another day? The Crucible by Arthur Miller portrays a society which corrupts itself through the belief of witchcraft. Witchcraft seemingly sets all the rules and standards for life and death and brings individuals against the court for their life or untimely death. Furthermore, there have been numerous individuals who go against their unjust government in order to protect a reputation, their own kind, and their beliefs.
In today’s society, the idea of witches have generally become a common topic. When Halloween arrives, we see people dressed up to mimic witches in fun spirit. However, during the Salem Witch Trials, witches were considered evil and against God, which caused fear to strike out amongst the people who religiously followed the Bible and believed in God. Thus, the accusations of citizens being involved with witchcraft struck out, and the persecutions began. When the topic of the Salem Witch Trials is mentioned, we think about how creepy it was and how the people were killed.
These three characters can be the most to blame for the cause of the spread and start of hysteria in Salem during the Witch Trials. The cause of the hysteria was caused by Reverend Hale, Abigail Williams and Judge Danforth. Three
The harsh reality of society’s callous notion for one to adhere to it’s ideals, leads to the prevailing
Annette Valdouin Ms.Dalton English 01/10/16 The Supernatural in Macbeth In the Shakespearean play Macbeth, Shakespeare includes supernatural elements such as the imaginary dagger, Banquo’s ghost, and the witches apparitions to give the audience insight as to how fragile Macbeth’s psyche is. In act two Macbeth is readying himself to kill King Duncan when he says, “Is this a dagger which I see before me,/The handle toward my hand?” (2.1.33-34).
When present, fear can often be exploited for one’s personal gain. The Crucible, a play written by Arthur Miller, which exemplifies the power of fear due to the imaginary idea of witchcraft in the small village of Salem. During the time this play was written, the United States was overcome by the fear of communism, which had led to the government accusing many innocent people for ridiculous reasons. Miller uses The Crucible to show how many of the accusations in the Salem Witch Trials, a similar event, often had underlying, selfish, and personal reasons behind them. In the play Abigail Williams, and Thomas Putnam’s take advantage of the pervasive fear in the village, allowing them to fulfill their selfish and exploitative motives which are what truly fuel the Salem Witch Trials.
The responder can develop a superior knowledge of dystopian societies through the comparison of Victor Kelleher’s novel ‘Taronga’ and Neil burgers Film ‘Divergent’, as both can be perceived as instable tales. This reveals the destruction of society’s values by one individual; they are compelled to confront the brutality, fear, and misuse of power that results.
How Paranoia and blame Affected the Salem Witch Trials and the McCarthy hearings In the 1690’s, a wave of fear for the devil washed over Salem, Massachusetts, resulting in the accusations of 200 supposed witches and the execution of 20. Almost 200 years later, after World War II, communists were highly feared. The strong urge to stay away from communists led to the McCarthy hearings where many innocent people were accused and tried for being communists. The Salem trials and the McCarthy hearings have many ties, the two closest being how paranoia highly affected the actions of individuals related to the cases and that the only way to save one’s self was to blame others.
Additionally, McCarthyism has had an effect on our society. McCarthyism taught the Government that fear is an effective tool for garnering support and maintaining political power. Both decades, the 50s and today, have resulted in severe loss of civil liberties and the exploitation of fear. The McCarthy Hearings were referred to as "witch-hunts" because of their similarity to the Salem witch trials. They both struck fear in the people due to the "guilty until you confess" attitude which ruled the courts.
The count of Salem gave an unfair trial, to those individual who contradict of witchcraft. The only deference, the people of Salem is to say the truth or lie. However the witch trail shows the difference between saying the truth and saying