Parkia Biglobosa Leaves Extract Case Study

INVITRO ANTI-INFLAMMATORY ACTIVITY OF PARKIA BIGLOBOSA FRUIT BARK EXTRACT

*Ukwuani AN and Ahmad HB

Department of Biochemistry, Faculty of Science, Kebbi State University of Science and Technology, Aleiro, Kebbi State. P.M.B, 1144.

Corresponding Email:[e-mail]

ABSTRACT

The invitro anti-inflammatory activity of various solvent fractions of Parkia biglobosa fruit bark was investigated using human red blood cell membrane stabilization, heat-induced hemolysis and protein denaturation methods. All the extracts of P. biglobosa fruit bark showed a concentration dependent increase in anti-inflammatory activity. The anti-inflammatory activity of the crude extract (60.8%, 58.3%, 78.2%) and last remaining aqueous extract (61.1%, 54.1%,

One kilogram (1.0 kg) of the fruit bark powder was extracted with hydromethanolic solvent (methanol (70): water (30)) for 72 hours. This was then filtered and evaporated to dryness in an oven at 45oc to give hydromethanolic crude extract (346g). 2.3 Phytochemical analysis

Preliminary phytochemical studies were conducted as per standard procedures described by Trease and Evan [7] and Harbone [8]

2.4 Solvent Partitioning of the Methanolic Extract

Hydromethanolic extract (100g) was redissolved in distilled water (200 mL) in a separating funnel and partitioned with n-hexane (4 × 200 mL). The resulting n-hexane phase was concentrated to dryness and known as hexane fraction which was stored in a freezer in an air-tight container. The resulting aqueous phase was further partitioned with chloroform, ethyl acetate and butanol respectively. The last remaining aqueous fraction was then evaporated to dryness and kept for further use in the freezer in an air-tight container [6].

2.5 Invitro anti inflammatory studies

2.5.1Preparation of Red Blood cells (RBCs)

Inhibition of albumin denaturation and anti-inflammatory activity of dehydrozingerone and its analogs. Indian J. Exp. Biol.1988; 26(10): 540 – 543.

Rajendran V, Lakshmi KS. In vitro and in vivo anti inflammatory activity of leaves of Symplocosco chinchinensis (Lour) Moore. Bangladesh J Pharmacol.2008; 3; 121-124.

Chou CT. The anti-inflammatory effect of Tripterygium wilfordii Hook on adjuvant induced paw edema in rats and inflammatory mediators release. Phytother Res. 1997; (11):152-154.

Charles OO, Peter AA, Nkemjika JO, Theophine CN, Anthonia CO, Chukwuemeka SN. “Acanthus montanus: An experimental evaluation of the antimicrobial, anti-inflammatory and immunological properties of a traditional remedy for furuncles”. BMC Complementary and Alternative Medicine. 2008; (8): 27.

Ferrali M, Signorni C, Ciccoli L, Comporti M. (1992).Iron release and membrane damage in erythrocytes exposed to oxidizing agents phenylhydrazine, divicine and isouramil. Biochemical Journal. 1992; 285 (1) 295-301.

Megha GC, Bhoomi BJ, Kinnari NM. In Vitro Antidiabetic and Anti-inflammatory activity of Stem Bark of Bauhinia Purpurea. Bull. Pharmaceut. Med. Sci. 2013; 1(2): 139 –