Animals: Take male albino rat 220-250 gram having age at least 2months old. Place them in animal house and supply it pellet diet and tap water. Animals are grouped in a number four. Each of the group contains 8 rats. Diabetes induced by injecting streptozocin bt introperitonial route of dose 60mg/kg .
Plant material and extraction 300gm dried powder of seed was weighed & taken in a aspirator (2.5L). Before placing powders into the aspirator, the jar was washed properly with acetone and then dried. 800ml of solvent i.e. methanol was added gradually. The container with its content was sealed & kept for 20 days with occasional shaking & stirring.
3.2.1 Ferric Chloride Test The sample extraction will be dissolved in 2 ml ethanol. A few drops of 10% ferric chloride solution will be added in test tube F. A green-blue coloration indicates the presence of a phenolic hydroxyl group (Bhatt et. al., 2011) 3.2.2 Sodium Hydroxide Test Few drops of 10% aqueous sodium hydroxide solution will be added in a test tube E with 2-3 ml of the extract. Formation of intense yellow color that became colorless on the addition of few drops of diluted HCl indicates the presence of flavonoids (Bello et. al.,
The test tubes with and without enzyme (control) were incubated at 370C for 1 hour. After incubation, the enzyme was denatured by heating the tubes at 1000C for 5 minutes. Both the test treated and untreated samples (control) were assayed for antibacterial activity by agar well diffusion method15. 7. Antibiotic Sensitivity
Their average weight (WA) was calculated. Percent weight variation was calculated. Average weights of the tablets along with standard deviation values were calculated.% weight variation = (WA–WI) x 100/ WA The weight variation between tablet ± 5 5. In-Vitro Drug Release Study The drug release study was performed by using type II dissolution apparatus (paddle type) in which phosphate buffer PH 5.8 at rotation speed 50 rpm and the temperature of the bath was maintained at 37.0+0.50C. The volume of the dissolution medium was kept 900ml and after a specified period of time 5ml of sample were taken and filtered by using whatman filter paper size no 1 ,after a filtration diluted with 500 micro liter in 10ml dissolution medium then analyzed quickly in UV double beam spectrometer at 243 nm.
(v) Sucrose preference test (anhedonia test) Sucrose preference (SP) test is a measure to evaluate anhedonic effect of CMS . In this test, rats were trained access to two bottles (water and 1% sucrose solution) freely for 7 days. The position of the 250-mL bottles containing sucrose solution or tap water was changed every day, to prevent location preference.Sucrose preference was expressed as percent of the volume of sucrose solution of a total volume of fluid (sacarose plus regular water). (vi) Data analysis The Statistical minitab 16.0 was utilized for statistical analyses. All data are presented as mean±S.E.M.
One hundered ml of representative sample of rumen liquor was brought immediately to the laboratory in a closed container tightened with a rubber stopper to sustain anaerobic condition during transport. Samples of rumen liquor were sieved between two layers of gauze to remove debris. Two 5ml duplicate liquor were separately taken and diluted five times by saline solution and lugol's iodine to fix and stain protozoan cell. 0.1ml of the diluted ruminal fluid was poured on a clean glass slide which was then covered by a clean cover slide. The total protozoa count / 1ml = average count in 30 field X 1173 (area of the cover slide X 50).
(2001) studied the various fractionation of the methanol extract of stems of Opuntia ficus-indica Mill. for anti-inflammatory action using adjuvant-induced pouch granuloma model in mice and identified β-sitosterol as an active anti-inflammatory compound. Lyophilized aqueous extract (100–400 mg/kg, i.p.) of the fruits of Opuntia dillenii (Ker-Gawl) Haw was evaluated for analgesic activity using writhing and hot plate test in mice and rat, respectively and also anti-inflammatory activity using carrageenan-induced paw edema in rats, the results exhibited dose dependent action (Loro et al., 1999). Hepatoprotective Opuntia ficus indica cladodes extract also have protective effect against liver damage induced in mice by anorganophosphorous insecticide, the chlorpyrifos (CPF).
aeruginosa, S. aureus, E. coli and C. Albicans. The aqueous and methanol extract of the plant are most effective against these microorganism . The Effect Of The Aqueous Extract Of Cyphostemma Glaucophilla Leaves On The Concentration of Total Plasma Proteins And Albumin Of Kwashiorkor Induced Rats Albino rats of either sex, weighing 100-110g were assigned into five Groups A, B. C, D and E of five rats each. Group A was the control and received rat feed and clean water, Groups B, C, D and E took protein deficient diet (corn meal) and clean water for 14days respectively.
Add 75 Ml Conc.HNO3 and heat the flask on a boiling water bath. On heating the flask sugar dissolves in nitric acid and copious evolution of nitrous fumes comes out. Discontinue heating. The reaction subsides in about 20 minutes. Pour the hot solution into a porcelain dish, wash the flask with 15 mL of nitric acid and evaporate the acid solution on a water bath