Paw Case Study

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Reagents and solutions: Carrageenan: 0.5%–2.0% (w/v) carrageenan solution Pour 500 ml of sterile 0.9% saline into a 1-liter beaker and give a stir. Slowly add 2.5 g (0.5%) – 10.0 g (2.0%) carrageenan powder to the beaker. Heat the solution to 90°C with continuous stirring, do not allow the solution to boil. Heating the mixture helps to dissolve the carrageenan; it takes less than 1 hour to dissolve all of the powder. Pour the solution into a clean and sterile 1-liter glass bottle. After cooling the solution to room temperature aliquot into sterile, 100-ml capped bottles. This solution is stable for at least one week when stored at 4°C. Methodology: Take male or female sprague-dawley rats with a body weight between 150 and 170g. The animals…show more content…
Calculate the increase in paw volume at 3 and 6 hr. The percentage increase in paw volume was measured by comparing the difference of the average values between treated group animals and control group animals is calculated at each interval of time. A dose-response curve is run for the active drugs to determine the ED50 values. The treated group animals show much less oedema than control group…show more content…
Croton oil induced ear oedema: In this method both rats and mice are used, usually male rats (Sprague dawley) of 80-60g are selected, whereas male Swiss mice weighing between 18-22g are selected. They are grouped according to the body weight. In this methodology, a total of 15mul containing an acetonic solution of 75microg of croton oil is applied to the inner surface of the right ear of each mouse. The animals are previously, anaesthetised with diethyl ether at a rate 0.02ml in rats and 0.01ml in mice. Methodology: Keep left ear untreated, control group receives the irritant solution, indomethacin is a standard or reference. Changing dose levels of test drug are applied to inner surface of the right ear of mouse that induces inflammation. When the test and standard drugs are given, sacrifice the animals by cervical dislocation. are Remove both treated and untreated ear and a plug of about 8mm diameter is removed from each of ear. Difference in the weight between two plugs is taken as the measure of oedematous response. Evaluation: This methodology is mainly used to detect the anti-inflammatory activity of steroidal compounds. The % anti-inflammatory activity is calculated

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