Reagents and solutions: Carrageenan: 0.5%–2.0% (w/v) carrageenan solution Pour 500 ml of sterile 0.9% saline into a 1-liter beaker and give a stir. Slowly add 2.5 g (0.5%) – 10.0 g (2.0%) carrageenan powder to the beaker. Heat the solution to 90°C with continuous stirring, do not allow the solution to boil. Heating the mixture helps to dissolve the carrageenan; it takes less than 1 hour to dissolve all of the powder. Pour the solution into a clean and sterile 1-liter glass bottle. After cooling the solution to room temperature aliquot into sterile, 100-ml capped bottles. This solution is stable for at least one week when stored at 4°C. Methodology: Take male or female sprague-dawley rats with a body weight between 150 and 170g. The animals are …show more content…
Croton oil induced ear oedema: In this method both rats and mice are used, usually male rats (Sprague dawley) of 80-60g are selected, whereas male Swiss mice weighing between 18-22g are selected. They are grouped according to the body weight. In this methodology, a total of 15mul containing an acetonic solution of 75microg of croton oil is applied to the inner surface of the right ear of each mouse. The animals are previously, anaesthetised with diethyl ether at a rate 0.02ml in rats and 0.01ml in mice. Methodology: Keep left ear untreated, control group receives the irritant solution, indomethacin is a standard or reference. Changing dose levels of test drug are applied to inner surface of the right ear of mouse that induces inflammation. When the test and standard drugs are given, sacrifice the animals by cervical dislocation. are Remove both treated and untreated ear and a plug of about 8mm diameter is removed from each of ear. Difference in the weight between two plugs is taken as the measure of oedematous
Cadet Eric Wiggins Date: 18 September 2014 Course Name: Chem 100 Instructor: Captain Zuniga Section: M3A Identification of a Copper Mineral Intro Minerals are elements or compounds that are created in the Earth by geological processes. The method of isolating metals in a compound mineral is normally conducted through two processes.
K.D.A. Saboia et al. , (2007) have been prepared the Bi4Ti3O12–CaCu3Ti4O12 {[BIT(X)–CCTO(100-X)]} composite powders through solid state reaction method and calcined in the range of 900 to 1020 ºC for 12 h. The as-prepared powders have modified in the form of thick film onto alumina ceramic substrate by utilizing screen printing. At 100 Hz, the value of dielectric constant (κ) of CCTO100 and BIT100 is 316.61 and 53.64 respectively. Conversely, the composite with X=20 % shows an unexpected dielectric constant of 409.71, which is around 20% higher in comparison with the CCTO.
The difference in this chemical and physical properties will aid in their separation. Processes like solubility, gravitational filtration and recrystallization will be used to separate the substances present in Panacetin. The melting and boiling point of the substances will help in concluding on which of these compounds will be presented at the end of experiment. Procedure and observation The Panacetin content was weighed approximately 3.0493g and transferred to the Erlenmeyer flask; 75ml of dichloromethane (CH¬2CL2) was added to the content. The dichloromethane (CH2Cl2) dissolved the sucrose, leaving the active unknown agent and aspirin behind.
Next, the test tubes were carefully cleaned with soap and water. Then five millilitres of sample “A” was placed in the test tube labeled “A”. This was then repeated with the next three samples. Then a few drops of each sample were placed on glucose/ketone paper. Each piece of glucose/ketone paper (with the sample on top) was compared to the label on the glucose paper bottle.
Using two separate aseptic pipettes, 250 µl of LB broth were added to each micro test tube and mixed gently. Likewise, using two separate, aseptic pipettes for each tube, 100 µl of solution was added to the appropriate agar plate. After, using a new loop for each plate, the solution was spread gently across their surfaces. Lastly, the plates were stacked, taped together, and labelled before placing them upside down in an incubator set at 37°C
The lab started off by measuring critical materials for the lab: the mass of an an empty 100 mL beaker, mass of beaker and copper chloride together(52.30 g), and the mass of three iron nails(2.73 g). The goal of this experiment is to determine the number of moles of copper and iron that would be produced in the reaction of iron and copper(II) chloride, the ratio of moles of iron to moles of copper, and the percent yield of copper produced. 2.00 grams of copper(II) chloride was added in the beaker to mix with 15 mL of distilled water. Then, three dry nails are placed in the copper(II) chloride solution for approximately 25 minutes. The three nails have to be scraped clean by sandpaper to make the surface of the nail shiny; if the nails are not clean, then some unknown substances might accidentally mix into the reaction and cause variations of the result.
While waiting, label the four petri dishes B lactamase - pGlo, B lactamase/ ampicillin -pGlo, B lactamase / ampicillin +pGlo ,and B lactamase/arabinase/ ampicillin +pGlo. After labeling the petri dishes, remove -pGlo and +pglo test tube containers from the ice cup and place them on the heat rack (at 42℃). Only leave the test tubes on for 50 seconds. After
Kim 1 Jaehee Kim Research & Presentation 2A Benard Kong March 23 2018 Osteochondrodysplasia of Scottish Fold cat breed One of the most crucial factors of purebred cats are the defining characteristics of each breed.
The Kirby-Bauer test is standardized in order to allow others to be able to reproduce the test since there are many factors that could affect the zone of inhibition. One of the standardizations is the medium used for the test, a Mueller-Hinton agar, which is different from the general mediums used in laboratories. The Mueller-Hinton agar plate is larger in size than standard agar plates so that multiple types of antibiotics are able to fit without interfering with each other (Slonczewski & Foster, 2015). The medium is thicker in depth (5 mm) and contains loose agar, which allows the antibiotics to diffuse out of the disk and into the media because if this test was conducted on a thinly poured agar plate, then the zone of inhibition would appear larger than it really is (Slonczewski & Foster, 2015). Another important factor about the Mueller-Hinton agar is that it doesn’t contain para-aminobenzoic (PABA) acid, which is usually present in most mediums.
EXPERIMENTAL RATIONALE Hydrocarbons are organic molecules consisting of hydrogen and carbon only1 and can be classified as saturated or unsaturated. A saturated hydrocarbon has the maximum number of hydrogens that can be present, given the number of carbons in the compound, for example, acyclic alkanes. A hydrocarbon is unsaturated if a hydrogen can be added1. This is a result of a hydrogen in the molecule being replaced, for example, with a double or triple bond or ring structure.
Examine the C Elegans to insure that the C Elegans have survived at the room temperature and continue to have multiple C Elegans surviving. Once this is done prepare the dilutions of all the subjects which we are testing. Start with 1% solution for Nitrate-N 100ml and move 10ml of the first well into the next. Fill the well with 90ml dh20 to reach 100ml. move 10ml of the second well to the third well.
Synthesis of Triphenylmethanol Using the Grignard Reaction and Acid Workup Amanda Sokol Partner: Jack Platacz TA: Edgar Reyes Cruz Lab: Tuesday, 1:40 - 4:30 PM PSH 334 March 17, 2023 Abstract: The purpose of this experiment was to synthesize pure triphenylmethanol by preparing and performing the Grignard reaction followed by an acid workup step. The two-week process combined various techniques used in lab this semester thus far with some new techniques; the first week involved making the Grignard reagent and its reaction with Benzophenone, while the second week consisted of hydrolysis as well as extraction and purification of the product through recrystallization. The solid product was also characterized through melting point and infrared spectroscopy.
Abstract – Methyl trans-cinnamate is an ester that contributes to the aroma of strawberry. It can be synthesized by an acid-catalyzed Fischer esterification of a methanol and trans-cinnamic acid under reflux. The solution was extracted to obtain the organic product, and evaporated residual solvent The yield was 68%, but there is some conflicting data regarding the purity. The melting point, IR, GC-MS indicate a highly pure desired product whereas 1H NMR shows there are unreacted reagents still present.
CLAIRE MUNTING 29/01/2018 Criterion C EFFECTS OF SURFACE AREA OF CALCIUM CARBONATE UPON RATE OF REACTION Calcium Carbonate Chips 1 Introduction: Within the current investigation, the effects of the surface area of Calcium Carbonate (CaCO3) in combination with Hydrochloric acid (HCl) upon its rate of reaction. CaCO3, commonly referred to as limestone, is an organic substance and is, in a sense, the crystallised “carbonic salt” of the element, calcium2. In addition to being a salt, the pH level of Calcium Carbonate is 9.91, and it is therefore, a basic substance, due to the fact that it is comprised of a pH level higher than 7, which is neutral3. HCl, however, is the bodily acid found in the stomach of human beings.
Lithium Chloride Causes Reduced Exploratory Behaviors in Rats Abstract In this pharmacological experiment, the affects of Lithium Chloride LiCl were studied using the Open Field Apparatus to assess for anxiety and fearful behaviors. The Sproang Dolleys were given an IP injection of LiCl then the total distance traveled was measured using the Open Field Apparatus. Other than the total lines crossed, no discernable affects were noted. The unequivocant data confirmed the initial hypothesis by proof in the results.