After 30 minutes, tubes were taken out and kept in ice-cold water for 30 minutes. These were centrifuged at 3000 rpm for 15 minutes. The absorbance of the supernatant was read at 540 nm at room temperature against appropriate blank. Blank consist of 1 ml distilled water, 0.5 ml of 30% TCA, and 0.5 ml of 0.8% TBA. TBARS values were expressed as n moles malonaldehyde (MDA)/mg protein.
The reaction absorbance of prepared mixture absorbance was measured at 760 nm in a spectrophotometer (Jenway UK). The total concentration of phenolic compounds in the extract was determined expressed as microgram of pyrocatechol equivalent (PE) per milligram of driedy extract. The total phenolics compoundscontent was determined as the pyrocatechol equivalent using an equation obtained from a standard pyrocatechol graph (y = 0.0057 x total phenols [μg PE/mg of dry extracts] - 0.1646,
The absorbance of the supernatant was read in spectrophotometer (UV-1601, SHIMADZU) at 540 nm against blank. Blank consisted of 1 mL distilled water, 0.5 mL of 30% w/v TCA and 0.5 mL of 0.8% w/v TBA. The results were expressed as nM of MDA (malondialdehyde) formed /hr/ mg of
RESULTS AND DISCUSSION Preformulation Studies The melting point of Metoprolol Tartrate was determined by capillary method and was found to be 121ºC (120 - 124ºC) as reported in literature, thus indicating purity of Metoprolol Tartrate. FTIR analysis showed that all the characteristic peaks of Metoprolol Tartrate and mixture of Metoprolol Tartrate with excipients were present in the range of reported frequencies; which shows that drug and excipients were compatible with each other. DSC thermogram of Metoprolol Tartrate alone showed endothermic peak at 120.73°C, corresponding to the melting point of the drug. The thermal curve of the Metoprolol Tartrate obtained indicated its crystalline anhydrous state, exhibiting a sharp endothermic peak at 120.73°C. The
The solution homogeneity expelled, by centrifugation for 10 min. The sample was centrifuged and separated into two layers, and took the top of the sample is injected for HPLC (11,12). Measured concentration in total lipid: The total fats balanced concentration of the pesticide getting by dividing the measured pesticide residue concentration in the overalll tissue sample by the decimal fraction of the sample that consisted of ether-extractable lipid. The total lipid content of each specimen was estimated from its total cholesterol & triglycerides levels by using a summation method. Analytical results for organochlorine pesticides were reported on a lipid-adjusted basis (nanograms per gram or parts per billion) (14).
DESIGN PSOW Ajit Rajendran 13H To Determine the Time Taken for a muffin paper cup to reach the ground, while Changing the Height of each experiment Introduction: In this experiment the aim is to determine the time taken for an empty muffin paper cup to reach the ground, by changing the height the empty cup is dropped from. Both variable mentioned are going to measured (height and time taken), when conducting the experiment. In order to have a fair experiment, certain factors will be kept the same throughout the experiment: the same paper cup will be used, the dimensions of the paper cup will be constant (where external factors do not affect the shape), the method in which the paper cup will be dropped. There will be a trend of the time
Preparation of ketoconazole loaded Proliposomes Ketoconazole (KTZ) loaded proliposomal gel formulations were formulated by method reported by Perret et al. 1991 with slight modification. Constant amount of drug was added to varying ratios of phophatidylcholine and cholesterol which constitute lipid component of 1mmol quantity. This lipid mixture was prepared in clean and dry, wide mouthed glass vials to which 400µL of absolute alcohol was added and after confirming the formation of homogenous dispersion, the glass vials were heated thermostatically on a water bath at 60-70oC with intermittent shaking. Add 160µL of double distilled water maintained at the same temperature to the transparent solutions formed, these upon cooling change to yellow translucent liquid/gel or white creamy proliposomal gel.
The apparatus consists of rings of reinforcing bar around a standard slump cone. The time taken by the concrete to completely flow is measured and then the length to which the concrete spread is measured. Fig 1. J Ring apparatus 3. L Box Test: This test is also done for checking the passing ability of the concrete.
The concentration of starch of the 1ml solution from test tube A is 500 after dilute with 9ml distilled water in test tube A. The 500 of starch solution become more dilute after added into 9ml test tube B, which become 50 . The concentration of solution in the few next test tube decreases in such way. The dilution factor, 5000 ratio to 50 is 1 : 100 in test tube B while the dilution factor in test tube C, 5000 ratio to 5 is 1 : 1000 and so