2. Animals: BALB/C mice, 15-20 g were housed in colony rooms with 12/12 hr light/dark cycle at 21±2°C and had free access to food and water. All animal experiments were carried out in accordance with CPCSEA guidelines, Ethical committee Acts. 3. Experimental protocol: Animals were acclimatized for a period of 1 week prior to the experiment.
Materials and Methods Experimental Design Forty (40) male albino rats of sprague Dawley Strain weighing (180 - 200 g) were included in the present study. The rats were obtained from the Laboratory of Animal Colony, Minya, Egypt and were housed in well aerated cages under hygienic condition and were provided commercial rodent diet and water ad libitum for one week for adaptation. Rats were housed in temperature controlled rooms (25°C) with constant humidity and 12h/12h light/dark cycle. After the adaptation period, the rats were divided into five groups (n=8 in each group) as follows: Group 1 (G1): considered as normal control group in which rats were fed on balanced diet for six weeks. Group 2 (G2): served as positive control group in which
Response surface methodology is a group of techniques that are used to study the relations between one or measured dependent factors (responses) and several input (independent) factors . The effect of concentration of the three selected variables, ammonium sulphate, yeast extract and 1,2 propylene glycol was studied by this method. The concentration ranges selected for the three factors are listed in (Table 4). All other factors (glucose 10 g/L, glycine 0.2%, temperature 28 °C, initial pH of medium 7.0, agitation rate 200 rpm, inoculum volume 2%) were kept constant. To calculate optimum values of selected three factors, a set of 20 experiments was generated using a 23 full factorial CCD, with six replicates at the centre point, was employed to fit a second order polynomial model.
5 mL of 3M sodium hydroxide, 5 mL of de-ionized water, and 15 mL of hexane were added to the reaction flask and stirred. The mixture was transferred to a separatory funnel, separated into an organic layer and water layer, and then drained. The water layer was washed twice with 10 mL of hexane. The organic layer was dried
The solution was stirred at room temperature for 8h. The solvent was blown out with nitrogen. The residue was added to 1 ml of water containing 0.1% TFA and purified on RP-HPLC. Massspec of the final product clearly indicates presence of RB modified on PEI by series of peaks matching different polymer compositions (see Fig. 6).
E. Discussion: In order to synthesize the polymer, Nylon 6,10, we had to complete a few steps to create the chemical reaction that combined sebacoyl chloride and hexamethylenediamine. First we measured the mass of the two graduated cylinders when they were empty, and measured it again after they were filled with sebacoyl chloride and hexamethylenediamine. We did this in order to find the measurements of the reactants. When we measured the graduated cylinder when they were emptied, one weighed at 10.99 grams while the other weighed at 10.94 grams. Even though they were the same kind of cylinder, I believe that a systematic error might have caused the second cylinder’s weight to be slightly affected, causing the weight to be lowered by 0.05 grams.
Finally, magnesium stearate (passed through a 60-mesh/250 micron screen) was introduced to the powder mixture. The final mixture was shaken manually for 5-10 min in a plastic bag. This powder was passed through the hopper of 16 station rotary tabletting machine and punched into tablets using 8mm s/c. the process is similar for all core formulations, which are prepared by direct compression technique. FORMULATION OF ENTERIC COATED TABLETS OF PANTOPRAZOLE SODIUM Formulation design: Pantoprazole enteric coated tablets were prepared using different polymers like HPMC 5cps (sub coat) AQOAT, CAP and KOLLICOAT MAE 30DP.
Glass plates were filled ¾ and the gel was covered with 100-500 µl Isopropanol in order to achieve an even surface. When the gel was polymerized, isopropanol was removed on the top of the running gel with water and thus, water was also removed as well. Then, stacking gel was prepared and its polymerization reaction was started with TEMED, and its solution was quickly poured on the top of the running gel. Therefore, a comb was inserted into the stacking gel before its polymerization, to form the loading wells. When the gel was polymerized, the inner chamber was filled with electrophoresis running buffer and the outer chamber was filled half.
Wash ether layer with saturated sodium chloride solution and retain ether layer. In a small 125ml Erlenmeyer flask, dry the ether solution over anhydrous calcium chloride. Add sufficient calcium chloride so that it no longer clumps to pellets added earlier on the bottom of the flask. Remove the solvent using a rotary evaporator and weigh product. Results 1 mole of benzoic acid (C6H5COOH = 122.12grams) reacts with 1 mole of methanol (CH3OH = 32grams/mole) to produce 1 mole of methyl benzoate (C6H5COOCH3 = 136.15grams) and 1 mole of water.
shows FTIR spectra of human blood serum treated with Urea of concentrations 100, 200, 300, 400, 500 mg/dl. Spectra show a series of bands, in the range of 4000 cm-1 and 400 cm-1, related to functional groups of proteins, lipids and carbohydrates and other inorganic materials present in the blood serum. Characteristic band for Urea can be seen approximately at 1085 cm-1. Wave Number (cm-1) Fig.1.(a). FTIR spectrum of human blood serum treated with Urea of concentration 100
The dried roots of Inula racemosa were pulverized and sieved with 100 ~ 200 mesh. The herb powder was placed into a glass bottle. Ultrasound-assisted extraction was carried out in an ultrasonic cleaner RK102H (Bandelin sonorex, Germany). The powder of Inula racemosa was extracted three times under the following conditions: the ratio of material to solvent was 10:1, undergoing ultrasonic treatment 30 minutes at 25 °C, 100 kHz /450 W.31 Before large extraction, a small-scale extraction experiments were carried out: 95% ethanol and ethyl acetate as the extractive solutions was investigated, respectively. The extraction efficiency was evaluated according to the percent content of AL and IS contained in the dried roots of Inula racemosa and calculated
Once the gel hardened, .5X TBE (44.5 mM Tris base, 44.5 mM boric acid, and 1.0 mM EDTA) was added just until the gel was covered with the TBE buffer. Each sample was loaded into the gel as well as 10 μL of DNA size markers (1kb ladder, New England Biolabs) into a separate lane. The gel was allowed to harden at room temperature and then electrophoresed at 100 volts for 75 minutes. Using a UV imager, a photo was taken of the resulting traveled DNA fragments in the gel. Results Table 1.