Petri Dish Lab Report
Based off the data that was collected, it is safe to say that if the dish contained the pGLO plasmid, the E. Coli growed. If there was no pGLO in the dish, then the only way E. Coli could grow is if it did not contain ampicillin which is the antibiotic. The only way that the E. Coli could grow is if the dish contained ampicillin and arabinose. Since arabinose is essentially and key that turns on the fluorescent glowing, it is essential to have in order to make the bacteria glow. Since no pGLO was present in one dish and ampicillin was also present, the bacteria were unable to grow.
Discussion
The four petri dishes that were tested were +pGLO Lb/amp, +pGLO LB/amp/ara, -pGLO LB/amp, and -pGLO LB. The two dishes that had +pGLO meant that they contained the pGLO plasmid. The two
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The reason we had the two controls were to see if we could successfully genetically transform E. Coli with the pGLO plasmid. In the beginning of the lab, we were able to predict that for the bacteria to grow, they needed the LB broth for nutrition and a gene that was resistant to ampicillin which is an antibiotic that would kill the E. Coli. In order for the bacteria to glow, they needed arabinose to activate the araC gene. After the experiment, it is safe to say that the hypothesis can be accepted. In the first dish, +pGLO Lb/amp, the bacteria was able to grow sing the pGLO plasmid was present and had the gene that was ampicillin resistant. But, since no arabinose was present, the bacteria was unable to glow. In the second dish, +pGLO LB/amp/ara, the bacteria was able to glow and grow since there was the pGLO plasmid which contains the gene that is ampicillin resistant, and the sugar arabinose which activates the araC gene which allows the bacteria to glow. In the third dish, -pGLO LB/amp, the bacteria did not glow or
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