Pglo Case Study
First, label one micro centrifuge tube +pGLO and another –pGLO. Using a sterile transfer pipet, transfer 250µl of competent cells (E. coli + CaCl2) into each tube and place them in crushed ice. Examine the pGLO plasmid DNA solution with the UV light and note your observations. Pipet 10µl of pGLO plasmid into the +pGLO tube and mix, close and return it to the ice rack. Do NOT add plasmid DNA to the –pGLO tube. Incubate the tubes on ice for 30min and make sure to push the tubes all the way in. During this time, label 4 agar plates with:
+pGLO LB/amp, +pGLO LB/amp/ara, -pGLO LB/amp, and -pGLO LB, the nutrients and ampicillin should be integrated within the agar. Next, one of the most important steps, heat shock, is used to assimilate the plasmid
The Mabo Case Study
695 Words | 3 PagesWhen the Europeans first arrived in Australia, Indigenous Australians lost all their land rights. This was mainly due to the Europeans claiming that Australia was Terra nullius. Terra Nullius was a international law stating that if territory was not owned, it was to be given to the first nation to discover it and entitled to take over. The Europeans did not recognise the Aboriginals and Torres Strait islander people as the traditional owners of Australia and therefore took all there land rights. The indigenous people were then constricted by the terra nullius rule from 1788 to 1991.
Wlp Case Study Case Management
269 Words | 2 PagesCase management services were provided through an FTM (family team meeting) in Anaheim. Present at the meeting were WYP (Wraparound Youth Partner) Andy Ngo, WPP (Wraparound Parent Partner) Shannon Ortiz, WCC (Wraparound Care Coordinator) Jody Taylor, the youth’s probation officer, and the youth’s father.
Intercellular Gene Transfer Lab Report
1100 Words | 5 PagesThe Mut section is expected to have no growth as mutants require the amino acids leucine and valine to grow which is not provided in the minimal medium. Results Figure 2. Testing of mutant mixed with DNA, mutant bacteria and DNA on LB medium Growth was observed on the Transformed (Trsf) section and the Mutant (Mut) section but not on the DNA section. Due to human errors, the photo of our experiment was lost, but we have obtained similar results as from group1.1 and their photo is presented.
Unknown Bacteria
557 Words | 3 PagesIn the laboratory, identification of an unknown bacterium is often necessary. In the lab, a random sample consisting of three different bacteria was selected. The sample contained one gram-positive, one gram-negative paracolon, and one gram-negative coliform. The purpose of the experiment is to identify each of the three species that the mixture contained. After receiving an unknown mixture, the sample was streaked for isolation onto TSA, blood agar, and MacConkey plates.
Peo Case Scenarios
228 Words | 1 PagesPO had moderate participation in the group discussion and activities that included: understanding what appropriate behavior on th job is; sharing the importance of wearing appropriate clothes and looking the best at work. PO was engaged in cross talking at times, but was able to focus back on topic after reminding. PO shared his work experience with peers, stated that clothing has an effect on both the wearer and the observer. PO actively participated in the check-in activity, reported he is doing “Okay” in treatment, and that he has no urges or cravings to use. Group topic: Identifying High-Risk Situations PO was minimally participated in the group activities that include: learning the terms of high risk situation, early warning signs,
Case Study: Listo System
1284 Words | 6 PagesLesson 1 Case Studies SANGAY SHERPA International American University MGT 500: Organizational Behavior and Leadership Dr. Om Sitaula September 17, 2014 LISTO SYSTEM Listo system is one of the leading graphic service company in the world. This is a company that achieved success in high pace in early 1990’s. It deals with all the graphic related programming. There are various aspects that led this company to success such as technological advancement, rapid growth in technology. Meanwhile it faces challenges from the high and changing demand of customers, advanced competitors etc.
Lco Case Study
879 Words | 4 PagesWhat is Economics? Economics is the science regarding the production, distribution, and use of goods, services, resources and wealth. A large part of our economy comes from the influence and decision making of our government. The Canadian Government Established the LCBO to monitor and manage the sales and distribution of liquor across Ontario.
Glucose, Insulin And Diabetes Mellitus Lab Report
841 Words | 4 PagesTube 1 had 1 drop, tube 2 had 2, and each tube after had an additional drop until tube 5. Next, deionized water was placed in each tube. Tube one had 4 drops; tube 2 had 3 drops and the pattern continued until tube 5. After each tube was filled with the glucose and deionized water, the contents were mixed and centrifuged. After the tubes were centrifuged, any pellets formed during the process were removed.
Xo Case Study
975 Words | 4 Pages1. Discuss the moral principle being followed by the XO. XO (Hunter) is acting based not only on the orders given to him but he thinks also the consequences of the orders being given. He is acting with due respect for the good of the many and not to just set the fire for war; it is here where utilitarianism comes in. Utilitarianism acts where the greater value is between either pleasure or pain.
Effect Of Temperature On Amylase
1167 Words | 5 PagesDo the same for the other test tubes. Let the test tubes not be disturbed for about 3- 4 mins. Then add the Amylase solution to the Starch solution and start the stopwatch (immediately). After every 1 min take one drop from the test tubes and place then in the test plate that were
P & G Case Study
997 Words | 4 Pages1. Define acronyms CRP, EDI, OSB, ECR and explain. CRP stands for "continuous replenishment program". CRP was a process that P&G created in order to increase logistic efficiency. The process consisted of using electronic data interchange (EDI), which is an electronic system that transmits data instantaneously from one business to another.
P & G Company Case Study
835 Words | 4 PagesTask 4 D1. Under this task I will evaluate how the marketing techniques of P&G Company organization have been a success Branding: P&G Company has a unique logo as a company in whole along with its other products for example the head shoulders shampoo. The P&G Company logo is blue in color and consists of 26 tiny icons that represent each and every product produced by P&G Company. P&G Company has a reputation for value, low price and for being customer-focused.
Wear Safety Lab Report
288 Words | 2 PagesPut all 50 test tubes in an autoclave and sterilize for 47 minutes. Inoculate each of the 50 test tubes with a 400 nL of E. Coli, and place caps on the test tubes . Put a set in an incubator at 37oC. Let E. Coli grow for 24 hours. Place 25 test tubes labeled “2.4 GHz” in the incubator with the Wi-Fi router. Place other
Gm Case Study Case
1114 Words | 5 Pages1. The competitive advantage and the basis of same till about 1975? Ans- • GM was the holding association of 25 different auto associations which were under GM. They all were working independently under GM and in every practical sense battling with each other only for the same customers. They were generally conveying premium estimated autos for the rich and affluent clients.
Importance Of Dna In Forensic Science
1251 Words | 6 PagesFinally, the amplified DNA regions are compare using a gel. DNA Profiling
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