Pglo Transformation Lab Report
To begin, during this lab experiment, genetic transformation was successfully carried out. After observing the agar plates, it was found that only the plate with ampicillin and no pGLO plasmid did not grow any of the E.coli bacteria. All three of the other plates grew the E.coli bacteria, however it grew differently in each plate. In the control plate where the pGLO plasmid, ampicillin, and arabinose were not present, the bacteria grew in the pattern that it was spread in originally. In the two other plates, bacteria grew in colonies that eventually joined together due to prolonged time in the incubator. Although transformation occurred in both plates with the pGLO plasmid, only the plate containing arabinose sugar had fluorescent colonies when observed under UV light. …show more content…
This explains why in the plate with ampicillin but no pGLO plasmid, the bacteria was unable to grow. If the pGLO plasmid did not contain ampicillin resistance, E.coli would not have been able to grow in the agar plates, rendering the experiment pointless. Secondly, the results exhibited the fact that arabinose allowed certain colonies in the E.coli bacteria to glow under UV light. Essentially, the arabinose acted as an inducer that binded to the repressor in the inducible operon, allowing the RNA polymerase to transcribe the DNA and synthesize the GFP, causing the bacteria to grow. In this case, the plasmid itself is not fluorescent because it is a gene and genes in DNA cannot glow themselves. However, DNA can code for certain proteins, such as GFP that would cause the bacteria to glow after going through genetic transformation, explaining why the presence of arabinose sugar caused fluorescence. For this reason, it makes sense that the transformed bacteria is fluorescent, but the plasmid itself is
Aliivibria Lab Report
251 Words | 2 PagesThe purpose of this experiment is to create a complete genomic library of Aliivibrio fisheri through the use of the lux operon. The examination of the lux operon gene occurs through the extraction of the DNA of Aliivibrio fischeri and digest a large piece of DNA to smaller random pieces. The fragment of DNA will later be ligated together in plasmid. Plasmid acts as vectors to transport DNA from one organism to another. The DNA will then run through a UV-visible spectrophotometer to test the absorbance of the extracted DNA.
Pglo Transformation Lab Report
664 Words | 3 PagesThe hypothesis for this experiment was that transformed bacterial cells would grow on ampicillin plates and glow green when exposed to UV light. The rationale for this hypothesis was because the plasmid would code for ampicillin resistance and a green fluorescent protein, we would have the outcome explained in the hypothesis. 4. Our predictions were that for the standard protocol plates with agar and ampicillin, there would be growth and the colonies would glow under UV light. On the modified protocol on plates with agar and ampicillin where we changed the time of the heat shock, we expected less growth but the colonies would still glow.
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Escherichia Colo Lab Report
308 Words | 2 PagesTransformation in bacteria usually takes place when a bacterial cell accepts strange DNA and integrates to its own DNA. The transformation normally takes place within plasmids, which are tiny circular DNA molecules that have been separate from its own chromosome. The copies of the same plasmid range from 10 to 200 copies within a cell. These copies of plasmids may multiply when the chromosome replicate or multiply independently. One plasmid has a range of 1,000 to 200,000 base pairs.
Pglo Transformation Lab Report
680 Words | 3 PagesThe cells that undergo transformation can activate the GFP protein using a sugar called arabinose that is added to the cells' growth medium ("pGLO Transformation,"). Using the aseptic technique, transformed cells will grow on the nutrient agar plates with LB/amp, and appear white on the plates that do not contain the sugar arabinose (Urnowey et al., 2017). If bacteria with +pGLO plasmids are found on the plate with LB/amp/ara, growth will take place and it will glow green under UV light because of the presence of arabinose. Furthermore, +pGLO bacteria that contains the gene for GFP and are resistant to the antibiotic ampicillin, will survive and develop on the plate that has LB/amp. In the control plates, -pGLO bacteria that is susceptible to antibiotic ampicillin will not grow on the plate with LB/amp.
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Pglo Lab
1396 Words | 6 PagesTransformation was successful in the plates where the bacteria consumed the pGLO plasmid. In the first plate that the bacterium was plated on it included the LB broth and of ampicillin antibiotic (amp), 2 colonies were present. The second plate of bacteria was grown with the presence of LB broth, ampicillin, arabinose sugar (ara), and 22 colonies were observed. But a green fluorescent glow of the colonies was only present in plate 2. Plates 3 and 4 were the control plates.
Unknown Lab Report
1444 Words | 6 PagesCrystal violet was then added for 60 seconds before being washed off with water. The mordant, Gram’s Iodine, was added for another 60 seconds before getting washed off with water. The heat fixed smear was then washed with 95% alcohol until the wash ran clear, leading to the final step of adding Safranin for 45 seconds before being rinsed with water. The slide was finally blot dyed with bibulous paper before it was placed under a microscope to observe the color and shape of the bacterium. 2.2 Litmus Milk Reaction
Examples Of Pglo Transformation Lab Report
875 Words | 4 PagesIn this lab, the goal was to transform bacteria with genes that included fluorescence as well as antibiotic resistance that were taken from a jellyfish. Transformation is transferring a gene from one organism to another. Certain precautions had to be made before doing this lab since every step had to be done very quickly to prevent too much contamination. The first step in starting the transformation is to add the transformation solution into the +pGLO and -pGLO test tubes. After this is done, you put both tubes in ice and then put bacteria in both tubes.
Lb-Pglo Lab Report
678 Words | 3 PagesThe plate labeled LB/amp/ara: +pGlo had several surviving colonies like the other +pGlo plate, except this time the colonies were a green color and the glowed under a UV light. The glow would be a result of adding the sugar arabinose, which seems to be acting as an inducer in the operon of the bacterium. It can be considered an inducer because in the presence of the arabinose, the colonies glowed, but without it the colonies did not experience any change, leading to the belief that the gene that leads to the green fluorescence is normally turned off. To further the research on the effect of the arabinose on the E. coli, a sample of the bacteria in the LB/amp: +pGlo and placed it in a plate with arabinose lines across the bottom.
Pglo Lab Report
1189 Words | 5 PagesFrom Genetic Transformation, Dr. Michael Blaber wrote, “The pGLO plasmid contains an origin or replication, a selectable marker, and the gene for Green Fluorescent
Pglo Transformation Lab Write Up
984 Words | 4 PagesThis experiment was tested on two conditions: +pGLO and -pGLO. +pGLO has the plasmid and -pGLO does not have the plasmid. Along with those conditions, ampicillin is added in the agar plate to destroy the non-transformed cells because scientist want to select only the transformed bacterial cells and the experiment is efficient at ten percent. To resist the antibiotic, the bacteria needs Beta lactamase that provide the enzyme. This allows the bacterial to break down the antibiotic and grow and with the plasmid.
Elodea Leaves Lab Report
275 Words | 2 PagesInstructions were read. Materials were provided. Purpose was understood: Observe plasmolysis in the Elodea leaves. We observed the plant cells under the microscope, and we roughly sketched an image of what we saw onto our lab worksheet. Then we added two drops of ten percent salt solution to the leaf slide and put it back under the microscope.
Kirby Bauer Diffusion Test Lab Report
624 Words | 3 PagesThe sterile cotton swab was inserted in the S. epidermidis culture and twirled around to obtain a specimen. The entire plate was inoculate with the swab from top to bottom, to achieve a lawn of growth. The dry forceps was used to remove the antibiotic disk into the appropriate spot on the plate. This process was repeated for the all antibiotics with aseptic technique being used. The plate was incubate with lid up on the bookshelf at room temperature for 48 hours.
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305 Words | 2 PagesWhat is happening to the particles in the water as it is being heated? How does this impact the state? When heat is added to a substance, the particles in the water vibrate faster. As the particles started to vibrate faster, the space between atoms increased.
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