Pglo Transformation Lab Report

Introduction: Transforming a gene or genetic information from one organism into another with the hopes that if done successfully the organism with the new DNA will be given new traits is a method known as genetic transformation (Rafter). Genetic transformation is used quite frequently in today’s world, form medicine to agriculture.

Genetic engineering is changing the DNA code to express different traits. A plasmid is a circular piece of DNA that contains important genetic information. Recombinant DNA is the product after inserting your desired genes. The genes we hoped to insert in the pGLO lab were the GFP gene and the ampicillin resistance gene. GFP was needed so that we would tell if the ampicillin resistance gene had been properly placed when the bacteria glowed under a UV light. The purpose of this lab was to perform a procedure known as genetic transformation which allowed us to genetically engineer E. Coli to be ampicillin resistance.

Everything can change from one substance to another, but the creation and the end of some things never happen .On a beautiful day on the fourth of December in 2015, the class of SNC1D5 started a lab on different kinds of molecules. The reason why we did this lab was to investigate the chemical and physical changes between different substances. Substances can change from one substance to another that have alternate physical and chemical properties by chemical reactions or with reactants or with heat. A physical change mixes up molecules but doesn’t hurt their inner structure. An example of physical change is ice turning into water due to heat because it didn’t change the substance’s material. A chemical change occurs when there’s a new arrangement

Chemical transformation, electroporation or particle bombardment is the typical method of construct into a host cell. Conjugation: The easiest illustration is to consider this as a version of bacterial sex. In conjugation the two bacterial cells connect, and the male donates a piece of DNA to the female. The piece of DNA was excised from a bacterial chromosome. The pieces are called plasmids.

Tn 4351 was originally isolated from bacteroides fragilis [30] . The transposon was successfully introduced into Cytophaga succinicans, Flavobacterium meningosepticum, Flexibacter canadiansis, Flexibacter strain SFI and Sporocytophaga myxococcoides by conjugation [25]. Tn 4351carries two antibiotic resistance gene. One of the codes for resistance to erythromycin and clindamycin which is expressed in bactroides but not in E.Coli. The other gene codes for resistance in tetracycline and is expressed in aerobically grpwn E. coli, but not in anaerobically grpwn E. coli or in bacteroides. The transposon of Tn4351 was originally detected in E. coli which carried an unstable chimeric plasmid, pSS-2. The mobilization of pSS-2 from onestrain of E. coli

We deal with two different strains of E. coli in this lab i.e. Standard E. coli which lacks resistance plasmid and strain containing a resistance plasmid which has genes that are protecting it from

a. What does the term vector mean in relation to infectious diseases? In relation to infectious diseases, a vector is any organism that carries, facilitates and transmits a pathogen into the body of another organism, often referred to as the host. The vector is not responsible for causing the disease in itself, but rather serves as a temporary reservoir for the pathogen before transmitting it to the host. b.

They tested how the temperature would affect the rate of reaction. This was observed by the amount of time it took for the solution to change colors. For many chemical reactions there is an optimum temperature at which the chemicals will react with each other. As was found in their experiment, the temperature affected the rate of reaction. (Deoudes, 2010).

The data observed and recorded in this lab shows that the concentration of miracle gro’ does affect the growth rate and germination speed of black eyed peas. The data is shown through two graphs and two data tables. The control group in this experiment is the seeds with a 0% concentration of miracle gro’, therefore the seeds with just water. The experimental groups are different concentrations of miracle gro’ including a 10%, 15%, 20%, 25%, and 30% concentration. The variable in this experiment is the amount/concentration of miracle gro’. On day one no seeds germinated. By day two, seeds in the control group, 15% and 25% experimental groups had germinated. On day two the experimental group with 25% concentration of miracle gro’ had the most seeds

Introduction For two days, on the 14th and 15th of April, a field excursion to Hastings Point, New South Wales was conducted. At Hastings Point, topography, abiotic factors and organism distribution were measured and recorded, with the aim of drawing links between the abiotic factors of two ecosystems (rocky shore and sand dunes), the organisms which live in them, and the adaptations they have developed to cope with these conditions. Within these two ecosystems, multiple zones were identified and recorded, and this report also aims to identify the factors and organisms associated with each zone. Lastly, using data and observations from the past, predictions for the future of the rock pool ecosystem were made.

The next test used the test tubes labelled “cold” and , one again using a piece of liver and five milliliters of hydrogen peroxide with both being placed in the ice bath, both held vertical with a test tube clamp. After five minutes were up using a timer, the two tests were conducted. The test involving the boiling water had five milliliters of hydrogen peroxide poured into it. Meanwhile, the five milliliters of hydrogen peroxide was poured into the test tube labelled “cold”. After both tests, explanations were made about the

Therefore, Green bacteria overcome low light conditions by absorbing light at longer wavelengths since longer wavelengths can penetrate further into the deep water. What are

These expressions of thought are ambiguous to the reader, which is disappointing since the scientific explanations of genetic transfer were explained in clearly. Although lacking creative writing style, the article provides effective visual aid for a teen audience to be engaged and inquiring to learn more about the issue. The diagram of a bacterial cell offers readers a comparison of bacterial chromosomes with that of plasmids. The cell does not include any other organelles to confuse or distract the student.

purpose the propose of this experiment was too see if the chemical reaction of a enzyme can be made faster. Hypothesis I think that a warm environment would be best to make an enzyme’s reaction faster. because a protein can move faster in heat.

After experiment on microscope under oil immersion, I learned that my Unknown is gram positive. Under the lens, the bacteria appears in purple color. Its morphology is cocci arranged in cluster. However, during decolorizing process, I put too much alcohol to the crystal violet-iodine complex making the color overly removed. That led to the result of my gram positive has slightly redish