Step 8: Pour hot water into a large bowl and place the glass jar in it. Step 9: Leave the glass jar in the hot water for an hour and shake the jar gently sideways from time to time to stir the leaves. Step 10: Prepare chromatography strips by measuring and cutting 13mm x 130mm rectangular
After which the digestions were examined by gel electrophoresis. The samples were run on a 50 mL 0.9% (w/v) agarose gel in 1X TAE buffer at 100 V until the leading track dye traveled 2/3 the distance of the gel. The gel was then soaked in GelRed for 20 minutes and examined under UV light. To prepare the digestions 10 μL of each digestion was mixed with 2 μL of 6X track dye in a micro centrifuge tube. 12 μL of 1 kb DNA ladder and each digestion was run on the
This helped us realize that a drippy faucet was an accurate clock. Lastly, we created sundials by tracing a protractor and then marking off intervals of 15 degrees, which were the time intervals starting with 6AM on the left side and ending with 6PM on the right. Then we stuck the screw through the paper in the middle of the bottom straight line. Thursday in lab, we first found the spring constant of our spring hooked onto our stand by timing the period it took for a 50g weight to oscillate back and forth. We timed the oscillation ten time and then found the average which was .77 seconds.
The two plastic containers were divided to which one contains which bean size. The beans that were selected must have been completely whole with no dents or abrasions. Five beans were placed in each section of the plastic container which means there were 20 beans in the two plastic containers. Then 12 female Callosobruchus maculatuss were sucked out of the main plastic container using an aspirator which was designed to suck in each Callosobruchus maculatus. The females were then separated into two groups of six and a group of six female Callosobruchus maculatuss was inserted into each plastic container.
1. Start off with the cut up tomatoes along with the tomato sauce, followed by the water. 2. Next, let that simmer for 5 to 7 hours, and within 15 minutes of serving, add your brown sugar, tobasco sauce, if desired, and kidney beans. Conclusion Sign post and Summary: In conclusion, today we gathered our ingredients, combined them, and then finally cooked those ingredients.
Experiment #7: Column Chromatography of Food Dye Arianne Jan D. Tuozo Mr. Carlos Edward B. Santos October 12, 2015 Abstract Column chromatography is the separation of mixture’s components through a column. Before proceeding with the column chromatography itself, a proper solvent system must be chosen among the different solvents. The green colored food dye is the mixture whose components are separated. The ammonia: 1-butanol (1:1) solvent was the appropriate solvent to use for the column chromatography of food dye because it exhibited the properties of a good solvent system. A total 8 colored eluents were collected.
primarily each isolate was cultured in broth medium and incubated on 37 C. overnight. Suspensions of any isolates were prepared that turbidity equal to 0.5 McFarland standards(OD=0.1). Each bacterium had been swabbed with those suspensions on Muller Hinton agar medium. Four wells were created (6mm in diameter and the distance of 25cm from each other) with sterile cork borer. Each well was poured with 100 μl clear supernatant liquid of any concentration.
A timer was set for 20 minutes once the potatoes were placed in the beakers. The potatoes were removed from the beakers after the 20 minute period. Each potato was weighed once more on the electronic balance scale to obtain the final mass. The data was recorded for each potato and the amount of sucrose they were placed in. The experiment was only done once.
After the second experiment (F2 ) was complete he found that 3/4 peas were yellow and the green pea had reappeared. Mendel had a hypothesis that there had to be some kind of factor (gene) that controlled the color of the peas. Mendel had concluded that there had to be two kinds of factors dominant and recessive factors. In the first experiment (F1) the yellow was completely dominant and it seemed the green factor had gone away. But in the second experiment (F2) the green gene had reappeared in one of the four peas in the offspring so in the second experiment the three of four peas the yellow gene was dominant and the other pea had the green recessive
We went to the salt marsh at Wrightsville Beach, North Carolina. Starting at the water line we measured 100 meters into the salt marsh. At every 10 meters we stopped and laid a quadrat down, which is just a square made out of pipe. We counted the number of each species and organisms we saw in each quadrat and also measured the height of each plant. We repeated these steps until we got to the 100 meter mark, which took us eleven quadrats total.
In this experiment, we devided each of the different beans. We used mug, black, and coffee beans. By placeing a plate for each bean, We where able dividing each the mug, black, and coffee beans into each plate. After dividing each bean we labled the name and group so we can better understand the difrence between the beans. The meathod of division is very simple and organized, alows to diffrentiate the difrence between each bean.
Fill the fourth well with 90ml dh20 to reach 100ml.Move 10 ml of the fourth well to the fifth well. FIll the fifth well with 90 ml of dh20 to reach 100ml. Start with 1% solution for Fluoride 100 ml and move 10ml of the first well into the next. Fill the well with 90ml dh20 to reach 100ml. move 10 ml of the second well to the third well.
Fill one planter with 200 ml of Miracle-Gro® Potting Mix, fill the second planter with 200 ml of Miracle-Gro® Seed Starting Potting Mix, fill the third planter with 200 ml of Miracle-Gro® Organic Potting Mix, fill the fourth planter with 200 ml of with Sunbury Christian Academy Soil. Measure .25 grams of seed with a balance, then place seeds one centimeter down into the soil of each of the planters. Label all of the planters as to identify the soil in each planter with a black Sharpie® Extreme black marker. Water the grass two times a day, once at 8 a.m. and again at 12:30 p.m. with 1.5 ml of distilled water using a disposable pipet. After the 8 a.m. watering, place the planters into the greenhouse.
Quinn Nguyen Floating Leaf Disk Photosynthesis Lab Conclusion: Graph your results for all 3 trials on one graph. Label the graph, both axes, and provide a legend to distinguish each trial. What was the rate of photosynthesis for each variable? How many leaves floated per minute? Rate of photosynthesis (leaves/min) Spinach: (2-0)/25 = 0.08 Lettuce: (10-5)/25 = 0.2 Poinsettia: (0-0)/25 = 0 What was the role of the sodium bicarbonate in this experiment?
32 100 μL of afore-prepared sample solution and the mixed reference standard were diluted 100 times with ethyl acetate. 50 μL of these dilution solutions were separated on the TLC plate coated with SNISG. The plate was developed with petroleum ether: ethyl acetate (4:1) and the movement of solvent was usually controlled at 1 cm from the upper edge. After completion, the plate was dried until no solvent smell remained. It was sprayed with an ethanol solution containing 10% sulfuric acid, and heated at an infra-red drier until obvious color came up, as shown in Fig.2 (B.ab).