Cell Cytotoxic Concentration (PHB)

The possible explanations and changes to make are similar to the previous questions. Conclusion and Future Experiment 18. The identity of the product and unknown were 4-tert-butylbenzyl phenol ether and tert-butyl phenol respectively. The key to making this discovery was the melting point and TLC results! The substitution reaction was successful but not fully effective.

Since our protein has seven histadine amino acids and no other amino acids present so the protein should have high affinity, in this way we will use column binding instead of batch binding to purify protein. Flow the mixture through the column. Save small amount of eluate. 3. Wash beads with His Binding Buffer.

If the amino acids be existent in the H2A+ generate initially, the titration with a strong acid and a strong base determination lead to the formation of the neutral zwitterion form (HA+/-) previously acquiescent the anionic usage (A-). The titration curve determine illustration two buffer regions and two inflection points specifying the equal points in the titration. The titration curve for a 25.0ml aliquot of the protonated form of 0.100 M glycine (H2A+) with 50.0 mL of 0.100 M HCl solution in note that the change around the another equivalence point is greatly less important than the primary

It can been seen that there are two distinct bonds, between two oxalates (94.06°, 93.56°, 90.61°, 94.30°) and within a singular oxalate (81.30°, 82.63°). The reason these bond angles vary within this octahedral complex are a result of lone pair repulsion creating slight variations among the bonds. Color Determination: To explain the coloring of Potassium Tris(Oxalato)Ferrate(III) Trihydrate, we must take into account λmax. Potassium Tris(Oxalato)Ferrate(III) Trihydrate had a λmax of approximately 395 nm, and formed yellow green crystals. When we look at a color wheel it can be seen that a substance with a λmax of approximately 395 nm would absorb violet light.

Properties of Ionic and Covalent Substances Lab Report Introduction The purpose of this lab was to determine which of the following substances: wax, sugar, and salt, are an ionic compound and which are a covalent compound. In order to accurately digest the experiments results, research of definitions of each relating led to the following information: ionic compounds are positive and negatively charged ions that experience attraction to each other and pull together in a cluster of ionic bonds; they are the strongest compound, are separated in high temperatures, and can be separated by polar water molecules. A covalent compound forms when two or more nonmetal atoms share valence electrons; covalent compounds are also

The pKa of this unknown weak acid is 4.0 and the Ka is 1.0 x 10-4 mol dm-3 while the molecular weight is 166.67 g mol-1. It is closely related to the ascorbic acid with a pKa of 4.10 and a Ka of 7.9 x 10-5 with a molecular weight of 176.12 g mol-1. Therefore it can be concluded that the unknown acid is ascorbic acid. Titration technique is best suited for this experiment because the end point and equivalence point can be distinguished by the physical changes which are the colour change of the mixture. We are also able to better control and determine the volume of NaOH in the burette needed to neutralise HCI, CH3COOH and the unknown acid.

The product (Cu(OH)2) was unstable and could reform Cu2+ and OH- ions due to its high solubility. This result can cause difficulties on separating Cu from the other metals in the AMD. Therefore, additional treatment, such as sulfidization, is required to prevent the occurrence of the reformation process

We measured how long it took for the high pH test tube to change color versus how long our control took. The Lugol’s Iodine test identifies for complex carbs. In our case, if the substance changed to a light brown color, the test was negative and the substance contained like glucose, and if the substance changed to a dark brown or black color, then the test was positive and the substance contained complex carbohydrates like starch. The substance with the high pH changed to a light brown at a time of 12 minutes and 49 seconds and the control changed to the same color at an earlier time of 11 minutes and 15

A) NaCl B) cholesterol C) C6H14 D) triglyceride E) fatty acid Answer: A Diff: 5 Page Ref: 31 13) Each of the following statements concerning hydrogen bonds is true except one. Identify the exception. A) Hydrogen bonds are strong attractive forces between hydrogen atoms and negatively charged atoms. B) Hydrogen bonds can occur within a single molecule. C) Hydrogen bonds can form between neighboring molecules.

Moreover, the desorpsi interaction of phosphate in the copolymer is more dominated by inter and intra-molecular interactions and hydrogen bonding between the functional groups in the copolymer with phosphate anion. At pH 10.05 solution is suspected OH- ion will take the H atom in -NH2, so that it make -NH- group because an excess of electrons. The negatively charged copolymer repel each other with a phosphate anion so that the amount of phosphoric is out of the copolymer