Cell Cytotoxic Concentration (PHB)

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ABSTRACT PHB is a promising eco-friendly substance favorable for medical use. PHB was produced from local isolate Bacillus thuringenesis in a conc. 4.1 g/l using 30 g/L Sugar-cane molasses (SCM) at pH 7.5 and incubation temperature 35oC. PHB biodegradation by soil microorganisms were completed after four weeks. Cell cytotoxicity testing is one of the critical factors affecting the biomedical application of polymers. 50% cell cytotoxic concentration (IC50) = 130 mg/ml while non-toxic concentration was 12.5 mg/ml. studying of direct contact application between biopolymer and peripheral blood lymphocytes resulting monomers have no toxic effect on the cells. INTRODUCTION Plastic is one of the most widely daily use requirements, whereas…show more content…
Two small additional peaks at δ = 0.8 and δ = 1.6 were found may be due to impurities present. 1H-NMR spectrum of PHA isolated from glucose or molasses media indicated characteristic signals of PHB, namely a doublet at 1.26 ppm, which is attributed to the methyl (CH3) group coupled to one proton while a doublet of quadruplet at 2.51 ppm due to the methylene (CH2) group adjacent to an asymmetric carbon atom bearing a single proton. The third signal at 5.25 ppm, which was attributed to the methine (CH) group. 1H-NMR is a very sensitive method for determining the domain size and miscibility, which is difficult to identify by conventional microscopic or thermal analysis (Kichise et al., 2002).The values of the chemical shifts as well as the assignments of the 1H-NMR signals, which appeared in the spectra are in agreement with results obtained by Kichise et al. (2002) and typically identical to peaks of the authentic PHB sample produced from Aldrich Company, which clearly shown that the extracted biopolymer from the B.thuringienesis in this study was poly-3-hydroxybutyric acid. These results were also confirmed by 13C NMR spectrum

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