Phlobatannin Lab Report

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extracts were treated with 2ml of glacidal acetic acid containing 1 drop of ferric chloride solution (0.1%). This was under large with 1ml of concentrated H2 504. A browning of the interface suggest a deoxylsugar characteristics of cardenolodes. A violet ring may appear below the broming, while in the acetic layer, a greenish ring may form gradually though out thin lager. Test for phlobatannin An aqueous extract of the plant sample was boiled with 1% aqeous hydrochloric acid and deposition of red precipate was taken as evidence for the presence of phlobatannins. Test for Terpenoids 5ml of each extract was mixed in 2ml chloroform, and concentrated H2 504 9 (3ml) was carefully added for form a layer. A reddish brown colouration of the interface…show more content…
5ml of the extract was pipetted into a 50ml flask and then 10ml of distilled water was added. 2ml of ammonium hydroxide solution and 5ml of concentrated amylachohol were made up to mark and left to react for 30min for colour development. This was measured at 505nm. Alkaloid determination using harborne (1973) method 5g of the sample was weighed into a 250ml beaker and 200ml of 10% acetic acid in ethanol was added and covered and allowed to stand for 4 hours. This was filtered and the extract was concentrated on a water bath to one quarter of the original volume. Concentrated ammonium hydroxide was added drop wise to the extract until the precipitation was complete. The whole solution was allowed to settle and the precipitate was collected and washed with dilute ammonium hydroxide and then filtered. The residue is the alkaloid, which was dried and weighed. Tannin determination by van-burden and robinson (1981) 500mg of the sample was weighed into a 50ml and shaken for 1 hour in a mechanical shaker. This was filtered into a 50ml volumetric flask and made up to the mark. Then 5ml of the filtered was pipitted…show more content…
The absorbance was measured at 120nm within 10min. Saponin determination The method used was that of Obadoni and Ochuko (2001). The samples were ground and 20g of each were put into a conical flask and 100cm3 of 20% aqueous ethanol were added. The samples were heated over a hot water bath for 4 hours with continuous stirring at about 550C. The mixture was filtered and the residue re-extracted with another 200ml 20% ethanol. The combined extract was reduced to 40ml over water bath at about 900C. The concentrate was transferred into a 250ml separatory funnel and 20ml of diethyl ether was added and shaken vigorously. The aqueous layer was recovered while the ether layer was discarded. The purification process was repeated. 60ml of n-butanol was added. The combined nbutanol extracts were washed twice with 10ml of 55%

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