Phosphorous is one of the three main nutrients essential in fruitful plant growth and is also a common component of many fertilizers used for commercial farming. Although many believe plants receive plenty of nutrients from what they absorb from the soil, this is not always the case; often, plants are growing in environments that lack sufficient nutrients, especially phosphorous (Mehrvarz et al. 2008). This has led to an increase in they buying of phosphorous based fertilizers to boost crop yield (Hammond et al. 2009).
Since the most main source of phosphorous is from phosphate rock, like many other natural resources, the supply will eventually near depletion (George et al. 2002). To combat the dwindling of phosphorous, it is essential to find
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Using a mortar and pestle to grind, 2 hole punches of the leaf were combined with 4 ml of 80% acetone. Special care was taken to ensure all acetone evaporated was replaced. The solution was then placed into a glass tube to centrifuge for five minutes at 3,000 rpm and 22 ֯C. 2ml of the resulting supernatant were transferred into another glass tube and were placed in a spectrophotometer to determine absorbance values at wavelengths 470 nm, 645 nm, 663nm, and 720 nm. This measurement was replicated 3 additional times for each treatment group. Between each wavelength, the spectrophotometer was blanked with 80% acetone to reduce background noise. The average stomatal density was collected by counting the number of stomata on four plants per treatment group. Using clear fingernail polish, an impression of the stomata on each leaf were made and taped to a slide with a 1mm by a 1mm grid which was then studied under a microscope to obtain the number of stomata per mm2.The average height of the plants was collected by measuring from the top of the soil to the tip of the leaf of eight plants per treatment group. All data collected was entered into excel and underwent a t-test to determine statistical …show more content…
The High stomatal density it the control and half phosphorous treatment groups was proven to be statistically insignificant while the low levels of stomatal density in the phosphorous deficient group was proven to be significant (Table 2). The phosphorous deficient treatment groups height was proven to be less than the half phosphorous treatment group and control groups heights and was also statistically significant (Table
The purpose of this experiment was to analyze the % phosphorous in Miracle Gro. Gravimetric Analysis was used to determine the content of phosphorous in this water soluble fertilizer. The overall goal of Gravimetric Analysis is to form the precipitate MgNH4PO46H2O and isolate it from the aqueous solution by vacuum filtration. In order to obtain the precipitate, a series of dissolution reactions and vacuum filtration were performed. First, Miracle Gro was dissolved with DI water and reacted with activated copper to remove coppers ions in the fertilizer.
All the test tubes contained in total 3 mL of solution. The following solutions’ concentrations in a tube were .1 mL of dye and 2.9 mL of water, .25 mL of dye and 2.75 mL of water, .5 mL of dye and 2.5 mL of water, 1.0 mL of dye and 2.0 mL of water, 2.0 mL of dye and 1.0 mL of water, and 3.0 mL of dye and 0 mL of water. These samples were tested by the spectrophotometer, and the absorbencies recorded. This whole process was completed twice and the absorbencies were averaged. Lastly, final concentrations and dilution factors were calculated by using the appropriate formulas.
In Burns Bog and Watershed Park, the light was blocked at certain times because of the cover of the trees. Light intensity affects the rate of photosynthesis as with more sunlight, more carbon dioxide can be produced for the plants to grow. The types of vegetation is affected by light intensity as when the amount of sunlight decreases, the growth decreases as well. This is why SWMA has more grass plants such as, Aster and Colonial Bent Grass. According to the Spearman's Rank Correlation Coefficient, the correlation coefficient of light intensity and Aster is 0.4.
Can Alka-Seltzer Act as a Buffer Against Acid Rain? Background: Acid rain is a product of rainfall being tainted by atmospheric pollution. This also means that it has high levels of hydrogen ions. When acid rain falls it has negative effects on natural environments such as aquatic life, plants, and infrastructure.
On page 117 paragraph 4 it says “ Phosphorous is found in fertilizer. In 1986, when detriment levels of phosphorous were discovered, water management becomes primary.” Since the sugarcane crop is grown so close to the Everglades the phosphorous found in fertilizer is getting into the
Introduction The purpose of this lab was to test one type of grass and four different types of soil in order to figure out which grass will grow tallest in 21 days. The hypothesis states that The Perennial Rye Grass will grow taller in the Miracle-Gro Organic Potting Mix than the other three grasses when given water over 21 days. The independent variable is the soil, the dependent variable is growth measured in height, and the control variables are as follows: 21 days of data collection, 1.5 mL of water a day, sunlight from 8 a.m. to 3 p.m. in greenhouse, temperature is about 70o F, 200ml of soil in each planter, five drainage holes made with a probe, .25 grams of seed, 1 cm into the soil, watered with 1.5ml twice a day and measured after the
Because carbon dioxide is absorbed by the plant during photosynthesis less carbon dioxide present in the chamber is a sign that photosynthesis is working. The four lights used for this experiment range across the light spectrum on both sides in order to test a wider variety of wavelengths. All lights will be placed directly on the spinach leaf at the same distance so as not to give any spinach leaf a different light intensity, which could affect the data. This experiment will be able to show which light, ranging across the light spectrum, will allow the Spinach to perform photosynthesis more efficiently.
However, after investigation through gel electrophoresis, the three kinds of plants were not identical. This relates to the
The plants with 200 ml topsoil were projected to have the highest average height according to the original hypothesis of increased plant growth due to an increase on the amount of topsoil added. Moreover, the original hypothesis was refuted as the plants with no topsoil in the control had a higher average height than the plants with 25 ml topsoil. The mixed red and green color on the leaves of the plants with 25 ml topsoil indicated possible deficiencies in phosphorus which might have slowed their growth. Moreover, the mixed red and green color on the leaves of the plants in both 100 ml and 200 ml topsoil also indicated a possible phosphorus deficiency which may have resulted in the slower growth of the plants despite having greater amounts of topsoil. The experimental results might have skewed due to the over fertilization of the radish seeds which might have led to excess nutrient levels which are unsuitable for radish plants as they require little to no fertilization for growth (Chase, 2018).
Jaspreet Singh Professor Paratore Biology 1 November 1, 2014 Spectrophotometry Identifying Solutes and Determining Their Concentration Statement of the Exercise or of the Problem The purpose of the lab experiment was to attain the following objectives: • Learning to Operate the Spectrophotometer • Construct absorption spectra for cobalt chloride and chlorophyll. Hypothesis If greater and higher concentrations of cobalt chloride are added to each solution then greater amounts of light would be absorbed by each solution. Thus a liner relationship will result in which the absorbance of a substance would be proportional to its concentration, which will be depicted, in a linear graph.
The principle involved in this test is the precipitation of phosphate which bores a yellow-colored solution and yellow precipitate. In the sample, neither a yellow-colored solution nor a yellow precipitate appeared which indicates the absence of phosphate in the sample. In the test for Purines, or Murexide test, the standard solution used was solid guanine. The reagents used were concentrated HNO3 and 10% KOH.
Phosphorus compound Phosphorus also exists as compound, occurring naturally on the earth crust as phosphates. Phosphates are the mostly used form of phosphorus such as Calcium phosphate; a natural rock used to make phosphate fertilizers. Phosphorus is alloyed with bronze. Phosphorus compounds play an important role in the use of energy in living things.
The 3 concentrations of enzymes were 0.5 ml, 1.0 ml, and 2.0 ml of turnip extract, while the substrate consisted of 0.1ml, 0.2 ml, and 0.4 ml of hydrogen peroxide. In a separate tube, the control was made up of turnip extract and guaiacol, known as the color reagent. This was recorded the absorbance every 20 seconds for 3 minutes.
Phosphorus metabolism : The endomycorrhizal symbiosis is beneficial for both fungi and plant. Fungi provide phosphorus to the plant while plant as a result give carbon to the fungi. But the phosphorus metabolism is the most important part of this association. Phosphorus is first absorbed by fungi from the soil and is stored in its cytoplasm while later fungi transport it to its vacuoles.
Fertilizers remove the nutrients of the soil damaging the soil and the local environment and after being mixed with the soil, gradually reduce the fertility of the soil. In the study of (Southland) using fertilizers consists of substances and chemicals like methane, carbon dioxide, ammonia, and nitrogen, the emission of which has contributed to a great extent in the quantity of greenhouse gases present in the environment. These facts are alarming and a serious step needs to be taken as soon as possible to avoid more severe consequences. This in turn is leading to global warming and weather changes. The use of fertilizers for growth and cultivation is keeping our stomach filled for now, but then if things keep on progressing the way they are, it won’t take long to see the times where there is lack of food, water, and health.