%Pasreceived is 5.8±0.008% and %Pdry 12.7±0.008 and %P2O5asreceived 6.63±0.008 and %P2O5dry 14.48±0.008. This shows that there are also other components present in the fertilizer sample that is Introduction The agricultural value of fertilizer samples depend on their total phosphate and water content. Natural phosphates are found in small amounts in all better types of soil and their presence is very important for plant growth. Phosphate content was determined as a precipitate magnesium ammonium phosphate hexahydrate.  Moisture determination is an important indicator of the fertilizer 's quality.
However the soil needs to have additional nutrients for successful plant grow. These can be provided by adding earthworms or compost. Earthworms and compost are both natural; they can both be used by the rural population as alternatives to provide nutrition to the soil and produce healthy crops and they are affordable. Spinach is a one of the few healthiest vegetables containing 20 measurable nutrients (Livingstone contributor, 2012). It has a very short development time and can be grown twice a year.
The use of fertilizers for growth and cultivation is keeping our stomach filled for now, but then if things keep on progressing the way they are, it won’t take long to see the times where there is lack of food, water, and health. In fact, nitrous oxide, which is a by product of nitrogen, is the third most significant greenhouse gas, after carbon dioxide and methane. Consequently, you can well imagine as to how harmful is the use of fertilizers for our environment and the ongoing use of fertilizers across the world
225 μl of solution G1 was added to 45 μl DNA in a 1 ml micro-centrifuge tubes and mixed briefly. The mixture was centrifuged at 15 000 g for 1 min at room temperature (Dragon LAB D2012 Centrifuge), then transferred from the micro-centrifuge tubes to collection tubes. The tubes were centrifuged for 60s at 15 000 g. The through flow was discarded. 300 μl of A1 solution was added to the column then centrifuged at 15 000 g for 1 min. The flow through was discarded.
One of the biggest problems are the soil is seasonable, it works best through the warm months because it has to break down and release nutrients into the air. Another problem farmers find with is the fertilizer takes time , the nutrients make time to break down and some people expect instant improvement and this soil will not do that for them(Chemical Fertilizer vs Organic). The nutrient level is lower than the chemical fertilizer, it can still do the same thing it just produces less nutrients in the long run. Just like the natural fertilizer the chemical fertilizer has its advantages and disadvantages. One of the biggest advantage is it has an instant improvement, humans have the natural desire to be impatient so most run to to the chemical fertilizer.
“Return an acre of farmland to productivity, or double yields on an already productive acre, and, other things being equal, you reduce by an acre the amount of virgin forest or savannah that will be stripped and cultivated. That may be the most important benefit of all” (Rauch). “We can't triple yields again with the technologies we're already using. And we might be lucky to get a fifty percent yield increase if we froze our technology short of biotech” (Rauch). “That the Green Revolution has saved not only many human lives but, by improving the productivity of existing farmland, also millions of acres of tropical forest and other habitat—and so has saved countless animal lives” (Rauch).
Medium and culture conditions Cells were grown in a 100-mL flask containing 25 mL into minimal medium supplemented with Eugenol for 2, 4 and 6 days incubation at 37 °C. The following Table 3. represents the composition of the modified minimal media used for biotransformation of eugenol (Muheim and Lerch, 1999). The pH of the medium was adjusted between 7.0 to 7.25 and autoclaved to obtain sterilized media for further
The culture was maintained on a nutrient agar plate/slants at 4°C and as glycerol stocks 40% at −70°C. Preparation of bacterial supernatant for synthesis of nanoparticles A loopful of the culture was inoculated into 250 ml sterile nutrient broth and incubated at 37°C for 24 hours. The
The organic agriculture techniques are known as ecological sustainable by • Improving soil structure and fertility through the use of crop rotations, organic manure, mulches and the use of fodder legumes for adding nitrogen to the soil fertility cycle. • Prevention of soil erosion and compaction by protecting the soil planting mixed and relay crops. •
Phosphorus metabolism : The endomycorrhizal symbiosis is beneficial for both fungi and plant. Fungi provide phosphorus to the plant while plant as a result give carbon to the fungi. But the phosphorus metabolism is the most important part of this association. Phosphorus is first absorbed by fungi from the soil and is stored in its cytoplasm while later fungi transport it to its vacuoles. Then these vacuolar components containing phosphoros are transported from outer mycelium of fungi to the plant.